Distribution of restriction endonucleases among some entomopathogenic strains of
            <b>
              <i>Bacillus sphaericus</i>
            </b>

Zahner, Viviane; Priest, Fergus G.

doi:10.1046/j.1472-765x.1997.00154.x

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…A previous study showed that many strains of L. sphaericus produce restriction endonucleases which could form a barrier to genetic manipulation [ 21 ]. The restriction enzymes and DNA methyltransferases (R-M systems) of the 11 Lysnibacillus strains were predicted by REBASE ( http://rebase.neb.com ).…”

Section: Resultsmentioning

confidence: 99%

Genome comparison provides molecular insights into the phylogeny of the reassigned new genus Lysinibacillus

Yuan

Rayner

et al. 2015

BMC Genomics

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BackgroundLysinibacillus sphaericus (formerly named Bacillus sphaericus) is incapable of polysaccharide utilization and some isolates produce active insecticidal proteins against mosquito larvae. Its taxonomic status was changed to the genus Lysinibacillus in 2007 with some other organisms previously regarded as members of Bacillus. However, this classification is mainly based on physiology and phenotype and there is limited genomic information to support it.ResultsIn this study, four genomes of L. sphaericus were sequenced and compared with those of 24 representative strains belonging to Lysinibacillus and Bacillus. The results show that Lysinibacillus strains are phylogenetically related based on the genome sequences and composition of core genes. Comparison of gene function indicates the major difference between Lysinibacillus and the two Bacillus species is related to metabolism and cell wall/membrane biogenesis. Although L. sphaericus mosquitocidal isolates are highly conserved, other Lysinibacillus strains display a large heterogeneity. It was observed that mosquitocidal toxin genes in L. sphaericus were in close proximity to genome islands (GIs) and mobile genetic elements (MGEs). Furthermore, different copies and varying genomic location of the GIs containing binA/binB was observed amongst the different isolates. In addition, a plasmid highly similar to pBsph, but lacking the GI containing binA/binB, was found in L. sphaericus SSII-1.ConclusionsOur results confirm the taxonomy of the new genus Lysinibacillus at the genome level and suggest a new species for mosquito-toxic L. sphaericus. Based on our findings, we hypothesize that (1) Lysinibacillus strains evolved from a common ancestor and the mosquitocidal L. sphaericus toxin genes were acquired by horizontal gene transfer (HGT), and (2) capture and loss of plasmids occurs in the population, which plays an important role in the transmission of binA/binB.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1359-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genome comparison provides molecular insights into the phylogeny of the reassigned new genus Lysinibacillus

Yuan

Rayner

et al. 2015

BMC Genomics

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“…The strains were screened for endonuclease activity as described by Zahner & Priest (1997). The endonuclease assay consisted of testing different aliquots of extract with 2 µg substrate (λ DNA and pBR322) in a total volume of 50 µl.…”

Section: Internal Transcribed Spacer (Its) Fingerprintsmentioning

confidence: 99%

Bacillus thermodenitrificans sp. nov., nom. rev.

Manachini¹,

Mora²,

Nicastro³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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“…SwissProt searches indicated that this was an S-layer protein, which is found in B. sphaericus. 67 In order to examine the role of the cell surface in generating spectral patterns, crude cell extracts from 20 strains that have previously been identified to belong to B. sphaericus [68][69][70] were analyzed by FIESMS. As observed previously S-layer proteins were found in the spectra.…”

Section: Biochemical Basis Of the Mass Spectral Information In Bactermentioning

confidence: 99%

High‐Throughput Microbial Characterizations Using Electrospray Ionization Mass Spectrometry and Its Role in Functional Genomics

Vaidyanathan

Goodacre

2005

Identification of Microorganisms by Mass Spectrometry

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Distribution of restriction endonucleases among some entomopathogenic strains of Bacillus sphaericus

Cited by 7 publications

References 5 publications

Genome comparison provides molecular insights into the phylogeny of the reassigned new genus Lysinibacillus

Genome comparison provides molecular insights into the phylogeny of the reassigned new genus Lysinibacillus

Bacillus thermodenitrificans sp. nov., nom. rev.

High‐Throughput Microbial Characterizations Using Electrospray Ionization Mass Spectrometry and Its Role in Functional Genomics

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