Distribution of Red Blood Cell Alloantibodies Among Transfusion-Dependent β-Thalassemia Patients in Different Population of Iran: Effect of Ethnicity

Background: In Burkina Faso, red blood cell (RBC) transfusion remains the crucial anaemia treatment following chronic renal failure (CRF) as erythropoietin and its analogues are unavailable. However, blood group matching beyond the ABO and Rhesus is not common in Burkina Faso. Thus, alloimmunisation is a potential issue for transfused patients.Objective: Our study aimed to identify anti-erythrocyte antibodies in multi-transfused CRF patients at the Yalgado Ouedraogo Teaching Hospital, Ouagadougou, Burkina Faso.Methods: This cross-sectional study, conducted from October 2018 to November 2019, included CRF patients who had received at least two RBC units. We screened patients for the presence of RBC antibodies using three commercial Cells panels and identified antibody specificities for positive screenings using 11 Cells panels for an indirect antiglobulin test (IAT) in a low ionic strength microcolumn gel-card system.Results: Two hundred and thirty-five patients (45.1% female; average age: 41.5 years) were included. The median number of blood units received per patient was 10 (interquartile range: 5–20). The overall alloimmunisation rate was 5.9% (14/235). Antibodies identified included: anti-D (1 case), anti-C (1 case), anti-D+C (4 cases), anti-CW (1 case), anti-E (1 case), anti-S (1 case) and anti-Lea (1 case). In four positive patients, the specificity of the antibodies was indeterminate. No risk factors were associated with alloimmunisation.Conclusion: In Burkina Faso, screening for RBC alloantibodies should be mandated for patients at risk. The high rate of indeterminate antibodies suggests the need to develop a local RBC antibody panel adapted to the local population.

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“… 36 , 37 A similar hypothesis was assumed in some other countries with multi-ethnic groups, such as Iran. 39 …”

Section: Discussionmentioning

confidence: 99%

“…36,37 A similar hypothesis was assumed in some other countries with multi-ethnic groups, such as Iran. 39 In Burkina Faso, blood group antigen distribution is established for ABO and RhD within blood donors and patients. 40,41 There is no data about Rh subgroups or other important RBC antigens.…”

Section: Discussionmentioning

confidence: 99%

Red blood cell alloimmunisation in multi-transfused patients from an haemodialysis service in Burkina Faso

Nébié

Sawadogo

et al. 2022

Afr. J. Lab. Med.

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The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran

Far

Oodi

Amirizadeh

et al. 2021

Molec Gen & Gen Med

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Introduction The alloimmunization following blood transfusion can be life‐threatening. The Rh alloantibodies are one of the most common causes contributing to alloimmunization. This study aimed to evaluate the rate and causes of alloimmunization and to determine the Rh phenotypes and genotypes among sickle cell disease (SCD) and sickle thalassemia (Sβ). Materials and Methods Our study included 104 SCD and Sβ patients referring to Baghaei 2 Hospital of Ahvaz in 2019 using a non‐random simple sampling method. The blood samples were collected for Rh phenotypes, alloantibody screening and identification, and molecular tests. The SSP‐PCR and RFLP methods with the Pst 1 enzyme were used. Results The alloimmunization rate was 9.6% and 13.2% based on immunohematological tests and medical records, respectively. The main alloantibodies (90%) were anti‐Rh, and 40% of the patients had multiple alloantibodies. A significant correlation was found between gender and alloimmunization. The phenotypes of DCce (37.5%), DCcEe (24%), Dce (20.2%), and dce (5.8%) and genotypes of R1r (25%), R1R2 (20.2%), R1R1 (18.3%), and R1R0 (10.6%) were the most prevalent. The R1R2 was a frequent genotype in Sβ. Conclusion R0r′ and R1R0 genotypes were limited to our population in Iran. Due to the differences in RH genotypes between our population and others, the blood transfusion from other ethnicities increased our total alloimmunization rate.

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Thirty years of hemovigilance – Achievements and future perspectives

Vuk

Politis²,

Laspina³

et al. 2023

Transfusion Clinique et Biologique

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Distribution of Red Blood Cell Alloantibodies Among Transfusion-Dependent β-Thalassemia Patients in Different Population of Iran: Effect of Ethnicity

Cited by 4 publications

References 25 publications

Red blood cell alloimmunisation in multi-transfused patients from an haemodialysis service in Burkina Faso

Red blood cell alloimmunisation in multi-transfused patients from an haemodialysis service in Burkina Faso

The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran

Thirty years of hemovigilance – Achievements and future perspectives

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