Distribution of protein disulfide isomerase during maturation of pig oocytes

Ohashi, Yumi; Hoshino, Yumi; Tanemura, Kentaro; Sato, Eimei

doi:10.1111/j.1740-0929.2012.01030.x

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…Both proteins reduce oxidative stress and protein misfolding essential for embryo development [39]. PDI/TRX is involved in oocyte maturation, [40] gamete fusion [41] zona hardening and monospermy [42] as well as proliferation preventing inner cell mass apoptosis [43]. Also, addition of exogenous TRX protects against ROS [7].…”

Section: Introductionmentioning

confidence: 99%

PreImplantation factor (PIF) protects cultured embryos against oxidative stress: relevance for recurrent pregnancy loss (RPL) therapy

et al. 2017

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Recurrent pregnancy loss (RPL) affects 2-3% of couples. Despite a detailed work-up, the etiology is frequently undefined, leading to non-targeted therapy. Viable embryos and placentae express PreImplantation Factor (PIF). Maternal circulating PIF regulates systemic immunity and reduces circulating natural killer cells cytotoxicity in RPL patients. PIF promotes singly cultured embryos’ development while anti-PIF antibody abrogates it. RPL serum induced embryo toxicity is negated by PIF. We report that PIF rescues delayed embryo development caused by <3 kDa RPL serum fraction likely by reducing reactive oxygen species (ROS). We reveal that protein disulfide isomerase/thioredoxin (PDI/TRX) is a prime PIF target in the embryo, rendering it an important ROS scavenger. The 16F16-PDI/TRX inhibitor drastically reduced blastocyst development while exogenous PIF increased >2 fold the number of embryos reaching the blastocyst stage. Mechanistically, PDI-inhibitor preferentially binds covalently to oxidized PDI over its reduced form where PIF avidly binds. PIF by targeting PDI/TRX at a distinct site limits the inhibitor’s pro-oxidative effects. The >3kDa RPL serum increased embryo demise by three-fold, an effect negated by PIF. However, embryo toxicity was not associated with the presence of putative anti-PIF antibodies. Collectively, PIF protects cultured embryos both against ROS, and higher molecular weight toxins. Using PIF for optimizing in vitro fertilization embryos development and reducing RPL is warranted.

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Section: Introductionmentioning

confidence: 99%

PreImplantation factor (PIF) protects cultured embryos against oxidative stress: relevance for recurrent pregnancy loss (RPL) therapy

et al. 2017

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“…To evaluate the PKC role on ER distribution, MII oocytes and oocytes activated in the presence or not of PKC inhibitor (BIM 10 µM) were evaluated by immunofluorescence using the antibody anti‐ER‐associated protein, protein disulfide isomerase (PDI). PDI has been successfully used to mark ER organization in oocytes (Ohashi, Hoshino, Tanemura, & Sato, 2013). In MII oocytes, ER was observed throughout the cytoplasm and mainly concentrated at the oocyte cortex (Figure 2, a–c; a′).…”

Section: Resultsmentioning

confidence: 99%

Endoplasmic reticulum distribution during bovine oocyte activation is regulated by protein kinase C via actin filaments

Feitosa

Lopes

Visintin

et al. 2020

Journal Cellular Physiology

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Fertilization‐induced [Ca2+]i oscillations generally depend on the release of calcium ions from the endoplasmic reticulum (ER). Since ER is the main store of calcium ions, it plays an important role in oocyte fertilization. However, the mechanism of ER organization at oocyte activation is unknown. Here, we show that protein kinase C (PKC) is involved in ER distribution during bovine oocyte activation, but not involved in cell cycle resumption and spindle organization. Actin filaments were affected by PKC pharmacological inhibition. In addition, similar to PKC results, the actin‐depolymerizing drug cytochalasin B affected the ER distribution during oocyte activation. Specifically, we have demonstrated that ER organization during bovine oocyte activation is regulated by PKC possibly through its action on actin filaments regulation. Taken together, the results presented here provide further information on the pathway involved in the regulation of ER organization during oocyte activation and new insight into the functional role of PKC and actin filaments during this process.

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“…PDIs are chaperone molecules advantageous for the viability and immune protection of eggs and early embryos of Pacific oyster, and it is up-accumulated in good quality oyster oocytes [ 19 ]. Previous studies reported that PDIs are involved in oocyte development [ 88 ] and sperm–egg fusion at fertilization [ 89 – 90 ], suggesting that also sperm–egg interaction are crucial determinants in oocytes quality and developmental success.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic features of Pecten maximus oocyte quality and maturation

et al. 2017

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The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping. To reach such a goal, a microarray analysis was performed by using a custom 8x60K oligonucleotide microarray representing 45,488 unique scallop contigs. First we identified genes that were differentially expressed depending on oocyte quality, estimated as the potential to produce D-larvae. Secondly, we investigated the transcriptional features of both stripped and spawned oocytes. Genes coding for proteins involved in cytoskeletal dynamics, serine/threonine kinases signalling pathway, mRNA processing, response to DNA damage, apoptosis and cell-cycle appeared to be of crucial importance for both oocyte maturation and developmental competence. This study allowed us to dramatically increase the knowledge about transcriptional features of oocyte quality and maturation, as well as to propose for the first time putative molecular markers to solve a major bottleneck in scallop aquaculture.

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Distribution of protein disulfide isomerase during maturation of pig oocytes

Cited by 8 publications

References 25 publications

PreImplantation factor (PIF) protects cultured embryos against oxidative stress: relevance for recurrent pregnancy loss (RPL) therapy

PreImplantation factor (PIF) protects cultured embryos against oxidative stress: relevance for recurrent pregnancy loss (RPL) therapy

Endoplasmic reticulum distribution during bovine oocyte activation is regulated by protein kinase C via actin filaments

Transcriptomic features of Pecten maximus oocyte quality and maturation

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