Distribution of proneuropeptide Y‐derived peptides in the brain of Rana esculenta and Xenopus laevis

Abstract

The distribution of proneuropeptide Y-containing perikarya and nerve fibers in the brain of Rana esculenta and Xenopus laevis was determined with antisera directed toward neuropeptide Y and the carboxyl terminal flanking peptide. The distribution of proneuropeptide Y-like immunoreactivity was similar in both anurans. In the telencephalon, immunoreactive perikarya were found in the olfactory bulb, all subdivisions of the pallium, the septum, pars lateralis of the amygdala, the nucleus accumbens, and the anterior preoptic area. In the diencephalon, labelled perikarya were detected in the ventromedial, ventrolateral and central thalamic nuclei, the magnocellular preoptic nucleus, the suprachiasmatic nucleus, the posterior tuberculum, and the infundibulum. Amacrine-like cells were stained in the retina. In the pretectal area, posterior thalamic neurons showed intense, Golgi-like immunostaining. In the mesencephalon, immunoreactive cells were found in the reticular nucleus, the anteroventral tegmental nucleus, the optic tectum, the interpeduncular nucleus, and the torus semicircularis. In the rhombencephalon, labelled perikarya were detected in the secondary visceral nucleus, the central gray, the nucleus of the solitary tract, the dorsal column nuclei, and the spinal nucleus of the trigeminal nerve. Immunoreactive nerve fibers were observed in all areas of the brain that contained labelled perikarya. The densest accumulations were found in the accessory olfactory bulb, pars lateralis of the amygdala, the ventral habenula, the posterior pituitary, the optic tectum, the interpeduncular nucleus, and the saccular nucleus. The distribution of proneuropeptide Y-like immunoreactivity in the anuran brain showed many similarities to the distribution described for the amniote brain.

Distribution of Neuropeptide FF-Like Immunoreactivity in the Brain of <i>Dermophis mexicanus</i> (Amphibia; Gymnophiona): Comparison with FMRFamide Immunoreactivity

“…Also different in Dermophis is the lack of NPFF-ir cells in the caudal rhombencephalon, in contrast with the situation in anurans and urodeles where a conspicuous cell population is present in the nucleus of the solitary tract [Crespo et al, 2003]. Of note, a similar situation was found in the solitary tract attending to NPY-containing cells that are absent in gymnophionans [Ebersole et al, 2001] and present in anurans and urodeles [Perroteau et al, 1988;Lázár et al, 1993].…”

Immunohistochemical localization and biochemical characterization of hypocretin/orexin-related peptides in the central nervous system of the frogRana ridibunda

“…Conversely, in rat, the suprachiasmatic nucleus is devoid of hcrt-positive cell bodies and exhibits only very few hcrt-immunoreactive fibers Cutler et al, 1999). Likewise, it has been reported previously that the frog suprachiasmatic nucleus is enriched with NPY-positive cell bodies (Danger et al, 1985;Lázá r et al, 1993;Tuinhof et al, 1994), whereas the rat suprachiasmatic nucleus only contains NPY-positive processes (O'Donohue et al, 1985;DeQuidt and Emson, 1986). In the frog diencephalon, three other nuclei contained hcrt-positive neurons, i.e., the anterior preoptic area, and the magnocellular and ventral hypothalamic nuclei.…”

Development of immunoreactivity to neuropeptide Y in the brain of brown trout (Salmo trutta fario)

“…In the inferior olivary complex of rat, both upregulation and down-regulation of NPY expression has been observed during development, and, in the adult, NPY immunoreactivity could be detected only in colchicinepretreated animals (Morara et al, 1997). An immunoreactivity-promoting effect of colchicine also has been noted in adult amphibians (Lázá r et al, 1993). Tuinhof et al (1994) also reported more intense staining in some larval nuclei than in adults.…”