Distribution of prolactin receptor in frog<i>(Rana Ridibunda)</i>dorsal skin during hibernation

Sengezer-Inceli, Meliha; Murathanoğlu, Osman; Castillo, Songül Süren; Sancar-Bas, Serap; Kaptan, Engin

doi:10.1556/abiol.62.2011.4.2

Cited by 3 publications

(1 citation statement)

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“…Epithelial sodium channel activity is regulated by PRL in A6 cells, and PRL has the ability to both stimulate and inhibit vascular function in vertebrates, including amphibians (Greenlee et al, 2014). In addition, PRL has a synergistic effect with growth hormone in amphibian metamorphosis, regulating the morphological and functional integrity of skin and influencing osmoregulation processes from the gills to the kidneys in amphibians; it plays an important role in the adaptation of amphibians during winter by providing morphological and functional integrity to the skin (Denver, 1996(Denver, , 1997(Denver, , 2013Pierce et al, 2007;Sengezer-Inceli et al, 2011;Clarke and Bern, 2012). In this study, the cDNAs of PRL and PRLR from the frog Rana chensinensis were cloned and their sequence features were analyzed.…”

Section: Introductionmentioning

confidence: 99%

Characteristic analysis of prolactin and its receptor genes fromRana chensinensis and expression pattern during metamorphosis

Wang¹,

Du²,

Wang³

et al. 2016

Turk J Zool

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In order to explore the role of prolactin (PRL) and the prolactin receptor (PRLR) during metamorphosis of amphibians, both cDNAs from the Chinese brown frog Rana chensinensis were identified. A PRL cDNA consisting of a 645-bp open reading frame and a PRLR cDNA consisting of a 1854-bp open reading frame were obtained. The amino acid sequence analysis indicated that there were 2 pairs of conserved Cys residues in both mature rcPRL and rcPRLR, and that their positions were conserved as well. Comparison of our results with those for other vertebrates implies that receptor recognition in anurans may not be pH-dependent. The relations of identity between rcPRL and other vertebrate PRLs resembled those of the identity between rcPRLR and other vertebrate PRLRs, suggesting that PRL may have coevolved with PRLR in R. chensinensis. In addition, rcPRL and rcPRLR mRNA was detected via RT-PCR in various tissues of R. chensinensis tadpoles. By using qRT-PCR, rcPRLR mRNA in the liver, skin, tail, and kidney tissue of different stages (stages 33-43) during metamorphosis was analyzed. The results showed that the expressional tendencies of rcPRLR increased throughout stages 33-43 in the four tissues, which was probably related to tissue remodeling.

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