Distribution of polysaccharides in the regenerating forelimb of the adult newt, diemictylus viridescens (Triturus v.)

Histochemical Identification of Sulfated and Non-sulfated Mucopolysaccharides in Regenerating Forelimbs of Adult Urodeles

“…Tissue periodic acid-Schiff reactivity obtained in the present investigation augments and extends Schmidt's [8] earlier observations. Non-sulfated mucopolysaccharide response was noted particularly in outer keratinized epithelial cells of normal and apical cap epithelium, the adepidermal reticulum, the secretions of dermal glands, sarco- Fig.…”

“…Marked quantitative and qualitative alterations in these metabolites occur as a result of forelimb amputation and subsequent regeneration [1,7,8,9]. During wound healing and redifferentiation polysaccharide reserves are depleted and mucopolysaccharide components of connective tissue, bone, and cartilage are broken down.…”

“…Prochondral cells actively engage in the production of a sulfated mucopolysaccharide for in corporation into the cartilagenous matrix [1,7]. Non-sulfated muco polysaccharides are prevalent in developing connective tissues, dermal gland secretions and the epidermal-dermal basement membrane; polysaccharide glycogen is present in late blastema cells, developing muscle, cartilage and the apical cap [8],…”

Changes in the levels and distribution of glycogen during regeneration of cutaneous wounds in Heteropneustes fossilis: a histochemical investigation

“…The reappearance of glycogen deposits in the epithelial cells and basal cells after epithelialization of the wound and the gradual increase in the basal cells up to 36 h and in the epithelial cells up to 72 h after wounding indicate that, after epithelialization, glycogen accumulated by these cells exceeds its utilization. The increased gradients of glycogen accumulation in epidermal cells have also been reported after injury in extensive studies on regenerating newt limb (Schmidt, 1960(Schmidt, , 1962, lizard tail (Radhakrishnan & Shah, 1973) and mammalian skin (Bradfield, 1951;Scothorne & Scothorne, 1953;Montagna, 1962;Lobitz & Holyoke, 1954;Hoopes & Michael, 1974;Rongone, 1977). The present study revealed the gradual appearance of glycogen deposits in the club cells which undergo vacuolization and attenuation (Mittal et al, 1978) after injury.…”

HISTOCHEMICAL AND ELECTROPHORETIC STUDIES OF ESTERASE IN THE REGENERATING FORELIMB OF THE ADULT NEWT, TRITURUS PYRRHOGASTER

“…For this purpose, several workers on regeneration ( KARCZMER and BERG, 1951;SAITO, 1960;SCHMIDT, 1962SCHMIDT, , 1963SCHMIDT andWEARY, 1962, 1963;SINGER et al, 1960;WOLFE and COHEN, 1963) have introduced histochemical method to see distribution and appearance of some enzymes in the regenerating tissues. Another line of study on enzymes in relation to development was pioneered by MAHKERT and HUNTER (1959), who demonstrated the existence of many molecular forms of esterase by means of starch-gel electrophoresis.…”