Distribution of poly(?-hydroxybutyrate) and poly(?-caprolactone)aerobic degrading microorganisms in different environments

Nishida, Haruo; Tokiwa, Yutaka

doi:10.1007/bf01458031

Cited by 262 publications

(138 citation statements)

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“…In conclusion, the slight decrease of the number of fungi in the earthworms casts compared to the population found in biosynthesol shows that earthworms regulate the number of fungi mostly by eating them. Among those fungi, Fusarium and Aspergillus strains known as able to bioassimilate PLA (Fusarium, Torres et al, 1996c) or other aliphatic esters (Aspergillus, Nishida et Tokiwa, 1993, Gonsalves et al, 1992 were found. It is thus probable that OLA is first degraded by those fungi and also by bacteria, and then earthworms eat them.…”

Section: Resultsmentioning

confidence: 99%

“…Torres et al (1996c) found a bacterium (Pseudomonas putida) and a fungus (Fusarium moniliforme) that were able to bioassimilate lactic acid oligomers (OLA) according to acronyms proposed by Vert and Guérin (1992). Furthermore, some strains of the fungus Aspergillus niger have been identified as able to degrade other aliphatic polyesters (Nishida andTokiwa, 1993, Gonsalves et al, 1992). Recent investigations (Alauzet, 1999) have shown that earthworms placed into biosynthesol (Bouché et al, 1998), an artificial soil modified from Abdul Rida and Bouché (1997) and containing only OLA as carbon source, are able to bioassimilate OLA after a previous digestion by micro-organisms.…”

Section: Introductionmentioning

confidence: 99%

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Microflora dynamics in earthworms casts in an artificial soil (biosynthesol) containing lactic acid oligomers

Alauzet

Roussos²,

Vert

2001

Braz. arch. biol. technol.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microflora dynamics in earthworms casts in an artificial soil (biosynthesol) containing lactic acid oligomers

Alauzet

Roussos²,

Vert

2001

Braz. arch. biol. technol.

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“…poly(ε-caprolactone) (PCL) = poly(β-hydroxybutyrate) (PHB) poly(ethylene succinate) (PES) poly(butylenes succinate) (PBS) poly(lactide) (PLA) (Nishida and Tokiwa, 1993;Pranamuda et al, 1995). PLLA-degrading thermophilic bacteria such as Brevibacterium brevis (Tomita et al, 2004), Bacillus smithii (Sakai et al, 2001) and Geobacillus thermocatenulatus (Tomita et al, 2004) have rarely been found in different environments.…”

Section: Thermophilic Bacteria Isolationmentioning

confidence: 99%

Characterization of poly(L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175

Hanphakphoom

Maneewong

Sukkhum

et al. 2014

J. Gen. Appl. Microbiol.

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on an emulsified PLLA agar plate at 50 C. Among the isolates, strain LP175 showed the highest PLLAdegrading ability. It was closely related to Laceyella sacchari, with 99.9% similarity based on the 16S rRNA gene sequence. The PLLA-degrading enzyme produced by the strain was purified to homogeneity by 48.1% yield and specific activity of 328 U·mg-protein-1 with a 15.3-fold purity increase. The purified enzyme was strongly active against specific substrates such as casein and gelatin and weakly active against Suc-(Ala) 3 -pNA. Optimum enzyme activity was exhibited at a temperature of 60 C with thermal stability up to 50 C and a pH of 9.0 with pH stability in a range of 8.5 10.5. Molecular weight of the enzyme was approximately 28.0 kDa, as determined by gel filtration and SDS-PAGE. The inhibitors phenylmethylsulfonyl fluoride (PMSF), ethylenediaminetetraacetate (EDTA), and ethylene glycolbis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) strongly inhibited enzyme activity, but the activity was not inhibited by 1 mM 1,10-phenanthroline (1,10-phen). The N-terminal amino acid sequences had 100% homology with thermostable serine protease (thermitase) from Thermoactinomyces vulgaris. The results obtained suggest that the PLLA-degrading enzyme produced by L. sacchari strain LP175 is serine protease.Key words: Laceyella sacchari; PLLA-degrading enzyme; Thermoactinomycetaceae; thermophilic filamentous bacteria IntroductionPoly(L-lactide) (PLLA); (C 3 H 4 O 2 )n is one of the aliphatic biodegradable polyesters derived from renewable resources such as corn, cassava, sugar cane, rice and potato through lactic acid fermentation, and it is also fully degradable by both microbial and enzymatic processes (Tokiwa et al., 2009;Gupta et al., 2007;Jarerat et al., 2006;Tokiwa and Calabia, 2006;Tomita et al., 2004). PLLA, as an environmentally friendly (eco-friendly or green ) product, has been developed on a large scale and is currently used for a wide range of applications, including packaging materials (Bhalla et al., 2007;Nolan-Itu Pty Ltd., 2002), medical applications (Jalil, 1990), agricultural products (Gross and Kalra, 2002;Sakai et al., 2001) and textiles.Most PLLA-degrading microorganisms are found in the bacterial order Actinomycetales, e.g. the families Pseudonocardiaceae (Pranamuda and Tokiwa, 1999;Pranamuda et al., 1997), Thermomonosporaceae (Sangwan and Wu, 2008;Sukkhum et al., 2009b), and Streptosporangiaceae (Sukkhum et al., 2009b). Apart from that, thermophilic bacteria in the families Thermoactinomycetaceae (Sukkhum et al., 2009b) and Firmicutes (Oda et al., 2000;Sakai et al., 2001), filamentous fungi in the genera Tritirachium and Paecilomyces (Sangwan and Wu, 2008), and yeast in the genus Cryptococcus have also been reported to be PLLA-degrading microorganisms.Many different types of enzymes are found in PLLAdegrading microorganisms, such as: protease from Amycola- Full PaperCharacterization of poly(L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175 (Received F...

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“…Polycaprolactone belongs to the group of synthetic polyesters, easily degradable by microorganisms [1]. It is used to produce implants and surgical sutures.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Polyhexamethylene Guanidine Derivatives Introduced into Polycaprolactone

et al. 2017

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Distribution of poly(?-hydroxybutyrate) and poly(?-caprolactone)aerobic degrading microorganisms in different environments

Cited by 262 publications

References 26 publications

Microflora dynamics in earthworms casts in an artificial soil (biosynthesol) containing lactic acid oligomers

Microflora dynamics in earthworms casts in an artificial soil (biosynthesol) containing lactic acid oligomers

Characterization of poly(L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175

Antimicrobial Activity of Polyhexamethylene Guanidine Derivatives Introduced into Polycaprolactone

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