Distribution of PCV‐2 DNA in the reproductive tract, oocytes and embryos of PCV‐2 antibody‐positive pigs

Bielański, A.; Larochelle, Renée; Algire, J.; Magar, Ronald

doi:10.1136/vr.155.19.597

Cited by 21 publications

(14 citation statements)

References 12 publications

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“…These results were consistent with those reported by Bielański et al (2004) and Madson et al (2008). We here suggest that PCV2 can induce lesions in host spermatocytes and oocytes prior to zygote formation.…”

Section: Discussionsupporting

confidence: 83%

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Germocyte lesions caused by porcine circovirus type 2b in Kunming mice

Yuan¹,

Pang²,

Gong³

et al. 2017

Polish Journal of Veterinary Sciences

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Porcine circovirus type 2 (PCV2) has been known as a causative agent of reproductive failure in the sow. In the present study mouse model was used to investigate PCV2 infection. In order to investigate whether PCV2 can induce lesions of spermatocytes and oocytes, 6 male and 6 female mice were each inoculated intraperitoneally with PCV2b, and 3 male and 3 female mice mock-infected with cell culture supernatant served as controls. Samples of testes and ovaries from PCV2b-inoculated and mock-infected mice were investigated using PCR, histopathological, ultrastructural and immunofluorescent histochemical methods at 14 and 21 day post infection (dpi). The study revealed that in the virus-inoculated mice leydig cells in testes and granulosa cells in ovaries were degenerated, and a small number of spermatocytes and oocytes showed apoptosis. Positive PCV2b antigen signals were also observed in these apoptotic cells. It can be suggested that PCV2 can cause lesions of spermatocyte and oocyte prior to zygotes formation in its host.

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Section: Discussionsupporting

confidence: 83%

“…Their findings indicated that PCV2 antigen was expressed in all types of ovarian follicles and corpora lutea in naturally PCV2-infected gilts. Bielański et al (2004) reported that PCV2 can invade to the zona pellucida of developing swine oocytes and embryos. They detected PCV2 DNA by PCR in the follicular fluid, oviductal cells and oocytes.…”

Section: Discussionmentioning

confidence: 99%

Germocyte lesions caused by porcine circovirus type 2b in Kunming mice

Yuan¹,

Pang²,

Gong³

et al. 2017

Polish Journal of Veterinary Sciences

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“…www.theriojournal.com Theriogenology 68 (2007) [896][897][898][899][900][901] In general, next to transplacental spread after viraemia, viral contamination of female or male gametes can also induce a vertical transmission of virus. For PCV2, using nested PCR assays, viral DNA was demonstrated in 11% of the cumulus oocyte complexes of sows with PCV2-antibodies [10] and intermittently in semen samples of experimentally and naturally infected boars [11][12][13]. Research on the infectivity of these contaminated gametes is limited.…”

Section: Introductionmentioning

confidence: 99%

Effect of a porcine circovirus type 2 infection on embryos during early pregnancy

et al. 2007

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The aim of the present study was to assess the effects of porcine circovirus type 2 (PCV2) on porcine embryos and their receptor sows during the first 21 days of pregnancy. Hatched blastocysts exposed to 10 5.0 TCID 50 PCV2 per ml (strain 1121, fifth passage PK15) and negative control embryos were transferred to PCV2-immune receptor sows at the 7th day of the cycle. Two weeks after transfer (D21), the receptor sows were euthanized and embryos were recovered. They were assessed macroscopically for viability and examined for viral antigen-positive cells by immunoperoxidase staining. The embryonic survival rate of the PCV2-exposed embryos (6.4%, 7 viable embryos out of 110 transferred) was significantly lower than the survival rate of the negative control embryos (65.4%, 34 viable embryos out of 52 transferred). All of the non-viable PCV2-exposed embryos (n = 9) displayed immunohistochemical positive signals for PCV2-antigen in degenerated tissues. In the PCV2-exposed embryos that were categorized as viable at D21, small clusters (n = 4) or no PCV2-positive cells (n = 3) were detected. The pregnancy results of the receptor sows that received PCV2-exposed embryos (1/5) were considerably different from the negative control receptors (2/2), with 3 out of 5 sows displaying a regular return to oestrus.In conclusion, it can be stated that PCV2 can replicate in embryos and might lead to embryonic death. In a small proportion of embryos, PCV2 exposure does not have a detrimental effect on embryo development before D21. #

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“…The results of a study of the distribution of PCV2 DNA in the reproductive tract, oocytes, and embryos of PCV2 antibody-positive pigs indicate that PCV2 may be associated with different tissues of the reproductive tract, but is unlikely to be associated with uterine-stage embryos. 4 Evidence of shedding of PCV2 in boar semen has been reported after both natural and experimental infection with PCV2. 16,18 Congenital tremor (CT) is characterized by tremor of the head and limbs of newborn piglets.…”

Section: Introductionmentioning

confidence: 99%

Association of Myocarditis with High Viral Load of Porcine Circovirus Type 2 in Several Tissues in Cases of Fetal Death and High Mortality in Piglets. A Case Study

et al. 2007

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Abstract. During a period of 1.5 months, a newly established pig herd experienced a high number of mummifications and stillbirths, a high neonatal mortality rate, and many piglets with congenital tremors or hind leg ataxia. After clinical and histological investigations, the submitted animals were divided into 4 groups: mummified or stillborn (N 5 6), live born with myocarditis (N 5 5) (average age 22.8 days), live born without myocarditis (N 5 14) (average age 20.0 days), and control animals from a different herd (N 5 5) (newborn). Statistically significant differences were observed in the mean porcine circovirus 2 (PCV2) load among the 4 groups in the liver (P , 0.0001). The presence of PCV2 antigen within the myocardial lesions was confirmed by immunohistochemistry. A high load of PCV2 DNA was observed in myocardium, liver, and spleen from mummified or stillborn piglets (.1 3 10 7 copies per 500 ng DNA), lower in piglets with myocarditis (.1 3 10 5 copies per 500 ng DNA), and even further lower in pigs without myocarditis (,1 3 10 5 copies per 500 ng DNA), whereas no PCV2 DNA was detected in the control animals. Myocardium, liver, and spleen were well suited for routine testing of fetuses and young piglets by quantitative real-time polymerase chain reaction. Neither porcine parvovirus nor encepaholomyocarditis virus was detected. These results indicate that the PCV2 infection might have been of etiological importance for the fetal deaths and piglet mortality observed in this herd.

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Distribution of PCV‐2 DNA in the reproductive tract, oocytes and embryos of PCV‐2 antibody‐positive pigs

Cited by 21 publications

References 12 publications

Germocyte lesions caused by porcine circovirus type 2b in Kunming mice

Germocyte lesions caused by porcine circovirus type 2b in Kunming mice

Effect of a porcine circovirus type 2 infection on embryos during early pregnancy

Association of Myocarditis with High Viral Load of Porcine Circovirus Type 2 in Several Tissues in Cases of Fetal Death and High Mortality in Piglets. A Case Study

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