Distribution of Pb and Zn in slices of human bone by synchrotron µ‐XRF

Zoeger, N.; Wobrauschek, P.; Streli, Christina; Pepponi, G.; Roschger, Paul; Falkenberg, Gerald; Osterode, W.

doi:10.1002/xrs.788

Cited by 40 publications

(35 citation statements)

References 12 publications

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“…However, the positions of maximum intensities were found to be 0-150 µm before the calcification front, depending on the sample analysed. As already discussed in a previous paper, 35 the local shift between the intensity maxima of the individual line profiles might be a consequence of the large sample thickness when compared to beam diameter (13 µm). The element-and matrix-specific information depths for different characteristic x-rays (e.g.…”

Section: Conventional Sr M-xrfmentioning

confidence: 62%

Determination of the elemental distribution in human joint bones by SR micro XRF

et al. 2007

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In humans the toxic trace element lead (Pb) mainly accumulates in the skeletal tissue where it resides for a long period. Although Pb is known to play a role in bone and cartilage diseases, the distribution of Pb in these tissues is mostly unknown. Therefore synchrotron‐radiation‐induced micro x‐ray fluorescence analysis (SR µ‐XRF) in various geometries was used to determine the distributions of Pb, Ca, Zn and Sr at the cartilage‐bone interface in human femoral heads and patellae. SR µ‐XRF results were matched with backscattered electron (BE) images providing information on structural features of the tissue. Conventional SR µ‐XRF at HASYLAB beamline L showed that Pb mostly accumulates in a zone of some micrometers around the transition between non‐calcified and calcified cartilage. However, the relatively large sample thickness did not allow more precise conclusions. Exploiting the 3D capabilities of confocal SR µ‐XRF and SR microfluorescence tomography at ANKA Fluo‐Topo beamline and HASYLAB beamline L, the spatial resolution could be improved and a highly specific accumulation of Pb at the tidemark, the calcification front between non‐calcified and calcified cartilage, was detected. It was found that Pb and Zn accumulation coincide at the tidemark. Since the tidemark plays an important role in osteoarthritis, the results may strengthen other authors' conclusions that Pb is associated with this joint disease. Although offering less spatial resolution when compared to micro‐tomography, confocal SR µ‐XRF is best suited for such studies. It facilitates the comparison of element maps with other imaging techniques like BE imaging. Copyright © 2007 John Wiley & Sons, Ltd.

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Section: Conventional Sr M-xrfmentioning

confidence: 62%

Determination of the elemental distribution in human joint bones by SR micro XRF

et al. 2007

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“…2e) is given. Bromine seems to be mainly distributed in connective tissue and vessel walls [27,28]. In normal liver tissue iron, copper and zinc distributions revealed no greater differences in their concentration throughout the measured area, and varied only in a small range (Fig.…”

Section: Sensitivity Of Spectrometer For Liver Tissue Analysismentioning

confidence: 90%

Iron, copper, zinc and bromine mapping in cirrhotic liver slices from patients with hemochromatosis studied by microscopic synchrotron radiation X-ray fluorescence analysis in continuous scanning mode

Osterode¹,

Falkenberg²,

Höftberger³

et al. 2007

Spectrochimica Acta Part B: Atomic Spectroscopy

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Iron (Fe) and copper (Cu) are essential metals in physiological cell metabolism. While Fe is easy to determine biochemically in histological slices, Cu and zinc (Zn) distribution is frequently critical in confirming the presence of an overload in disturbed Fe/Cu metabolism. To analyze Fe, Cu and Zn in a near histological resolution, energy dispersive microscopic synchrotron radiation X-ray fluorescence was applied.In normal liver tissue, after fixation and imbedding in paraffin, mean Fe, Cu and Zn concentrations were 152 ± 54, 20.1 ± 4.3 and 88.919.5 μg/g sample weight, respectively. No substantial, characteristic differences in their distribution were found in the two-dimensional scans. In slices from patients with hemochromatosis mean Fe, Cu and Zn concentrations were 1102 ± 539, 35.9 ± 14.6 and 27.2 ± 6.7 μg/g sample weight, respectively. Additionally, a significant decrease in phosphorus and sulphur concentrations existed. An increased Cu around cirrhotic regenerations nodules is mostly associated with a lymphocytic infiltration in this region. Analyzing concentrations of Fe in different regions of the samples show a clear negative dependency between Fe and Cu, Cu and Zn, but a positive one between Fe and Zn.Conclusion: With a focal beam size of 15 μm in diameter a resolution of the elemental distribution was achieved which is widely comparable with stained histological slices (20× light microscope). The analysis of simultaneous determined elements reveals metabolic differences between Fe, Cu and Zn in liver tissue from patients with hemochromatosis.

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“…Recent SYXRF applications include mainly geological samples (e.g. Lowenstern 1993;Hansteen et al 2000), but also biological material like human bones (Zoeger et al 2005), tree stems (Martin et al 2006) and human teeth (Barrea et al 2003). The SYXRF-analyses of fish otoliths (Tsukamoto et al 1998;Nakai et al 1999), bivalve shells (Thorn et al 1995) and corals (Allison et al 2001) highlighted its suitability for bio-mineralized aragonite.…”

Section: Introductionmentioning

confidence: 99%

Influence of temperature and salinity on the trace element incorporation into statoliths of the common cuttlefish (Sepia officinalis)

et al. 2007

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The use of statolith chemistry to trace migration pathways and distinguish populations of cephalopods is based on the assumption that the elemental composition of statoliths is influenced by physicochemical properties of the ambient environment. However, such influences have not been investigated experimentally up until now. This study presents the first microchemical analyses of cephalopod statoliths obtained from laboratory experiments under different controlled temperature and salinity conditions. Our results show that statolith chemical composition is strongly related to both salinity and temperature in ambient waters. The Ba/Ca ratio is negatively related to temperature and shows no relation to salinity. The I/Ca ratio is positively related to temperature and negatively to salinity. No Sr/Ca relation was found to either salinity or temperature, suggesting that the well-established proxy strontium is not as useful in cephalopod statoliths as in other biomineralized aragonites. Microanalysis of trace elements, however, shows an enormous potential for field studies on distribution, migration and stock separation of cephalopods. Furthermore, Synchrotron X-ray Fluorescence Analysis is introduced as a promising novel method for statolith analysis, providing a spatial resolution of typically 10-15 lm combined with detection limits down to 0.5 ppm.

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Distribution of Pb and Zn in slices of human bone by synchrotron µ‐XRF

Cited by 40 publications

References 12 publications

Determination of the elemental distribution in human joint bones by SR micro XRF

Determination of the elemental distribution in human joint bones by SR micro XRF

Iron, copper, zinc and bromine mapping in cirrhotic liver slices from patients with hemochromatosis studied by microscopic synchrotron radiation X-ray fluorescence analysis in continuous scanning mode

Influence of temperature and salinity on the trace element incorporation into statoliths of the common cuttlefish (Sepia officinalis)

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