Distribution of p53 binding protein 1 (53BP1) and phosphorylated H2A.X during mouse preimplantation development in the absence of DNA damage

Ziegler-Birling, Céline; Helmrich, Anne; Tora, Làszlò; Torres-Padilla, Maria-Elena

doi:10.1387/ijdb.082707cz

Cited by 72 publications

(73 citation statements)

References 49 publications

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“…Although there is some discrepancy in the literature with respect to the presence of phospho-H2A.X in mouse zygotes, our results in control embryos are in agreement with several published articles in which this histone modification was closely monitored (e.g. Wossidlo et al, 2010;Ziegler-Birling et al, 2009).…”

Section: Discussionsupporting

confidence: 92%

The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition

Fulka

Langerova

2014

Development

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The oocyte (maternal) nucleolus is essential for early embryonic development and embryos originating from enucleolated oocytes arrest at the 2-cell stage. The reason for this is unclear. Surprisingly, RNA polymerase I activity in nucleolus-less mouse embryos, as manifested by pre-rRNA synthesis, and pre-rRNA processing are not affected, indicating an unusual role of the nucleolus. We report here that the maternal nucleolus is indispensable for the regulation of major and minor satellite repeats soon after fertilisation. During the first embryonic cell cycle, absence of the nucleolus causes a significant reduction in major and minor satellite DNA by 12% and 18%, respectively. The expression of satellite transcripts is also affected, being reduced by more than half. Moreover, extensive chromosome bridging of the major and minor satellite sequences was observed during the first mitosis. Finally, we show that the absence of the maternal nucleolus alters S-phase dynamics and causes abnormal deposition of the H3.3 histone chaperone DAXX in pronuclei of nucleolus-less zygotes.

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Section: Discussionsupporting

confidence: 92%

The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition

Fulka

Langerova

2014

Development

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“…While G1/early S-phase embryos exhibited only a few gH2AX foci, the number increased drastically by mid/late S phase, declined again by G2 phase, and disappeared completely before M phase. Mitotic chromatin was heavily labeled with gH2AX, as reported before (Ziegler-Birling et al 2009). In Ring1 mÀz+ /Rnf2 mÀz+ embryos, we observed similar gH2AX patterns at G1/early S and mid-S phases (Supplemental Fig.…”

Section: Maternal Ring1/rnf2 Deficiency Delays Meiotic Maturation Andsupporting

confidence: 77%

Polycomb function during oogenesis is required for mouse embryonic development

Pósfai¹,

Kunzmann²,

Brochard³

et al. 2012

Genes Dev.

121

102

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In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei in reproductive cloning suggests that intergenerational inheritance of germline chromatin contributes to developmental proficiency after natural conception. Here we show that Ring1 and Rnf2, components of Polycomb-repressive complex 1 (PRC1), serve redundant transcriptional functions during oogenesis that are essential for proper ZGA, replication and cell cycle progression in early embryos, and development beyond the two-cell stage. Exchange of chromosomes between control and Ring1/Rnf2-deficient metaphase II oocytes reveal cytoplasmic and chromosome-based contributions by PRC1 to embryonic development. Our results strongly support a model in which Polycomb acts in the female germline to establish developmental competence for the following generation by silencing differentiation-inducing genes and defining appropriate chromatin states.

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“…10). This could be partly due to the fact that rather high levels of ␥-H2AX are present in wt embryos (36). Alternatively, this difference could be explained if the chromatin environment, which is thought to be globally rather different in the cleavage-stage embryo compared to that of an ES cell, is also a determinant in triggering the DNA damage response.…”

Section: Discussionmentioning

confidence: 99%

Monomethylation of Histone H4-Lysine 20 Is Involved in Chromosome Structure and Stability and Is Essential for Mouse Development

Oda

Okamoto

Murphy

et al. 2009

Molecular and Cellular Biology

284

358

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PR-Set7/Set8/KMT5A is the sole enzyme known to catalyze monomethylation of histone H4 lysine 20 (H4K20) and is present only in multicellular organisms that compact a large fraction of their DNA. We found that mouse embryos that are homozygous null mutants for the gene PR-Set7 display early embryonic lethality prior to the eight-cell stage. Death was due to the absence of PR-Set7 catalytic activity, since microinjection of the wild type, but not a catalytically inactive version, into two-cell embryos rescued the phenotype. A lack of PR-Set7 activity resulted not only in depletion of H4K20me1 but also in reduced levels of the H4K20me2/3 marks catalyzed by the Suv4-20h1/h2 enzymes, implying that H4K20me1 may be essential for the function of these enzymes to ensure the dimethylated and trimethylated states. Embryonic stem cells that were inducibly deleted for PR-Set7 passed through an initial G 2 /M phase, but the progeny were defective at the subsequent S and G 2 /M phases, exhibiting a delay in their cell cycle, accumulation at G 2 /M, massive DNA damage, and improper mitotic chromosome condensation. Cell cycle analysis after synchronization indicated that the defects were a consequence of decreased H4K20me1 due to the absence of PR-Set7. Most importantly, the lack of H4K20me1 also resulted in defects in chromosome condensation in interphase nuclei. These results demonstrate the critical role of H4K20 monomethylation in mammals in a developmental context.Posttranslational modifications (PTMs) on histones influence intra-and internucleosomal interactions and thereby contribute to the diversity in nucleosome and chromatin structure that impacts distinct genomic processes. Among these modifications, histone methylation had been considered to be relatively stable, but recent studies demonstrated that, similar to the other PTMs, it too is subject to regulation. Many methylating and demethylating enzymes that target different lysine and arginine residues have been identified. These enzymes have distinct specificities with respect to the methylation status (monomethyl, dimethyl, and trimethyl) of each residue. Furthermore, increasing numbers of proteins harboring the motifs that specifically recognize various methylated residues have been identified. These proteins, or effectors, mediate/regulate elaborate chromatin-based processes, such as gene expression, which are dictated by the presence of the PTMs. Thus, the catalysis/removal of PTMs and their recognition by effectors constitute an intricately designed system that is key to genomic integrity and function.One of the residues of histone H4 that can be monomethylated, dimethylated, or trimethylated is lysine 20. Recent comprehensive analysis of H4 modifications with top-down mass spectrometry in human and in Drosophila melanogaster cells revealed that the dimethyl group is deposited on the majority of total H4K20, indicative of the wide distribution of H4K20me2 on chromatin (18, 34). On the other hand, H4K20me1 and H4K20me3 are relatively few in abundance. The study a...

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Distribution of p53 binding protein 1 (53BP1) and phosphorylated H2A.X during mouse preimplantation development in the absence of DNA damage

Cited by 72 publications

References 49 publications

The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition

The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition

Polycomb function during oogenesis is required for mouse embryonic development

Monomethylation of Histone H4-Lysine 20 Is Involved in Chromosome Structure and Stability and Is Essential for Mouse Development

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