Distribution of oxidation enzyme eNOS and myeloperoxidase in primary open angle glaucoma

Abstract

Genetic factors and the influence of superoxide are known to play roles in the etiology of glaucoma. We evaluated the association between primary open angle glaucoma (POAG) and two polymorphisms in the epithelial nitric oxide synthase (eNOS) gene, and one polymorphism in the myeloperoxidase (MPO) gene. We enrolled 66 patients with POAG and 100 healthy volunteers in this study. The polymorphisms in the eNOS and the polymorphism MPO -463 G-to-A in the MPO gene were resolved by polymorphism polymerase chain reaction (PCR). There were no significant differences in the distribution of the eNOS intron -4 (P=0.481), eNOS promotor -786 (P=0.555), and MPO -463 (P=0.292) gene polymorphisms between the POAG patients and the volunteers (P-values=0.481, 0.555, and 0.292, respectively). None of the alleles from either gene differed between the groups (P-values=0.483, 0.554, and 0.183, respectively). Superoxide is closely related to glaucoma, and eNOS and MPO are two important enzymes in the free radical pathway. However, polymorphisms of the eNOS intron-4, eNOS promotor -786, and MPO -463 gene polymorphisms did not reveal significant differences between POAG patients and controls in our study. The use of these agents and other superoxide-related genes for clinical applications requires further investigation.

Association of polymorphisms of endothelial nitric oxide synthase (eNOS) gene with the risk of primary open angle glaucoma in a Brazilian population

“…In the overall analysis, we observed a marginal difference in the frequency of this polymorphism among cases and controls, which was not significant after adjustments for other variables of interest. These results are in accordance with the findings observed by other authors (Fan et al, 2010;Lin et al, 2005;Logan et al, 2005). The Glu298Asp polymorphism, in turn, has been associated with risk of ischemic shock (Berger et al, 2007), coronary spasm (Yoshimura et al, 1998), coronary artery disease (Colombo et al, 2003;Hingorani et al, 1999), myocardial infarction (Hibi et al, 1998;Shimasaki et al, 1998) and NAION (Sakai et al, 2007).…”

“…While some studies have implicated polymorphisms in the promoter region or inside the eNOS in the risk of POAG (Ayub et al, 2010;Liao et al, 2011;Tunny et al, 1998), other authors found no significant association (Fan et al, 2010;Lin et al, 2005;Logan et al, 2005). Interestingly, recent reports indicate that these different polymorphisms could interact with other exposure factors in modulating the risk of POAG, such as gender, use of postmenopausal hormones (PMHs), hypertension and smoking (Kang et al, 2010(Kang et al, , 2011.…”

Association of Polymorphisms of Tumor Necrosis Factor and Tumor Protein p53 with Primary Open-Angle Glaucoma

“…28 However, another promoter SNP (rs2070744) of NOS3 was not associated with POAG in two other studies. 69,70 In addition, the 27-bp repeat alleles in intron 4 of NOS3 were associated with POAG in British and Pakistani populations 70,71 but not in a Chinese population. 69 In the present study, we did not find any association between two NOS3 SNPs (rs2070744 and rs1799983) and POAG.…”

“…69,70 In addition, the 27-bp repeat alleles in intron 4 of NOS3 were associated with POAG in British and Pakistani populations 70,71 but not in a Chinese population. 69 In the present study, we did not find any association between two NOS3 SNPs (rs2070744 and rs1799983) and POAG. Of note, the minor allele T of rs3918226 in NOS3 was not found in our Chinese sample (Table 3).…”

Complex genetics of complex traits: the case of primary open‐angle glaucoma

“…A part of this problem is that molecular pathways have been used to work backwards to a genetic predisposition. As in the case of nitric oxide synthase 3 (NOS3) , different expression patterns were found in the aqueous humour of glaucomatous patients compared with matched patients 122,123 . However, when nucleotide polymorphisms that had been proven to be functionally important in the NOS3 gene were investigated, conflicting results were obtained 122,123 .…”