Distribution of orexin/hypocretin immunoreactivity in the brain of a male songbird, the house finch, Carpodacus mexicanus

Singletary, Kristan G.; Deviche, Pierre; Strand, Christine R.; Delville, Yvon

doi:10.1016/j.jchemneu.2006.12.002

Cited by 8 publications

(19 citation statements)

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“…This result is in line with similar observations from several studies in teleosts, amphibians, reptiles, birds, and mammals [Shibahara et al, 1999;Galas et al, 2001;Phillips-Singh et al, 2003;Kaslin et al, 2004;Huesa et al, 2005;Singletary et al, 2005Singletary et al, , 2006Nixon and Smale, 2007;. In contrast, orexins in specific cells of the adenohypophysis have been reported in human [Blanco et al, 2003], orexin-A in two species of anurans [Yamamoto et al, 2004;Suzuki et al, 2007], and orexin-A and orexin-B in the pituitary of two species of teleost fishes [Amiya et al, 2007;Suzuki et al, 2007Suzuki et al, , 2009.…”

Section: Distribution Of Orexin-ir Fibers In the Brain Of Vertebratessupporting

confidence: 90%

“…Thus, the situation found in turtles (Pseudemys) with abundant orexin-ir CSF-c cells closely resembles that observed in Neoceratodus , whereas lizards and Protopterus share a more restricted distribution of orexin-ir cells . The situation of orexin-ir cell bodies restricted to a single group (the paraventricular nucleus of the hypothalamus) has also been observed in different avian species [Ohkubo et al, 2002;Phillips-Singh et al, 2003;Singletary et al, 2006].…”

Section: Orexin-ir Neurons In the Brain Of Vertebratesmentioning

confidence: 79%

“…The antisera anti-orexin-A and anti-orexin-B (both of Santa Cruz Biotechnology) used in this study are raised against a peptide mapping at the C-terminus of orexin-A or orexin-B of human origin. This would explain why there were no differences in the yielded pattern of staining [Singletary et al, 2005[Singletary et al, , 2006 and only orexin-immunoreactivity, in general, could be investigated. In addition, regarding their amino acid sequence, the orexins do not have structural homology with other biologically active peptides [Shibahara et al, 1999].…”

Section: Discussionmentioning

confidence: 99%

“…Strikingly for osteichthyan fishes, lungfishes possess a well developed catecholaminergic (putative dopaminergic) cell group in the mesencephalic tegmentum that, because of its reciprocal connections with the telencephalic striatopallidal system, has been compared to the substantia nigra of amniotes [González and Northcutt, 2009]. These cells receive abundant orexin-ir terminal structures on their dendrites in mammals and birds [Bubser et al, 2005;Singletary et al, 2006;Balcita-Pedicino and Sesack, 2007]. The present observations, together with previous data in amphibians and reptiles, suggest that this relationship is likely a shared feature between lungfishes and tetrapods .…”

Section: Possible Relationships Between the Orexinergic And Monoaminementioning

confidence: 99%

“…Interestingly, the primary amino acid sequence of orexins is highly conserved across vertebrates [Sakurai et al, 1998;Shibahara et al, 1999;Alvarez and Sutcliffe, 2002;Ohkubo et al, 2002;Panula, 2009]. Thus, the distribution of orexin-like immunoreactive cells and fibers in the brain of different vertebrates has been analyzed by means of immunohistochemical techniques using antibodies against mammalian orexins [Shibahara et al, 1999;Galas et al, 2001;Ohkubo et al, 2002;PhillipsSingh et al, 2003;Kaslin et al, 2004;Huesa et al, 2005;Singletary et al, 2005Singletary et al, , 2006Amiya et al, 2007;. In general, the localization of orexin-ir cell bodies in particular forebrain nuclei and the widespread distribution of fibers were corroborated for all vertebrate groups studied, but when direct cross-species comparisons were made, using the same immunohistochemical approach, important variations were found in the precise distribution of orexin-ir cells and fibers [Singletary et al, 2006;Nixon and Smale, 2007;.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes Protopterus dolloi and Neoceratodus forsteri

et al. 2009

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Lungfishes are currently considered the closest living relatives of tetrapods and represent an interesting group for the study of evolutionary traits in the transition from fishes to tetrapods. The brains of lungfishes have received little attention in comparative studies probably due to the difficulty of obtaining these unique animals. In the present study the distribution of orexin (hypocretin)-like immunoreactivity was studied in the brain of the African lungfish Protopterus dolloi and the Australian lungfish Neoceratodus forsteri by using antibodies directed against the mammalian orexin-A and orexin-B peptides. Simultaneous detection of orexins and tyrosine hydroxylase or serotonin was used to assess the precise location of the orexins in the brain and to evaluate the possible influence of the orexin system on the monoaminergic cell groups. Although some differences were noted, a common pattern for the distribution of orexins in the two lungfishes studied was observed. In both species, most immunoreactive neurons were observed in the suprachiasmatic nucleus and dorsal hypothalamus. Only in Neoceratodus, however, were important cell populations found in the preoptic area and infundibular hypothalamus, whereas small numbers of faintly reactive neurons were present in the lateral septum and ventral striatum. Fiber labeling was widely distributed in all main brain subdivisions, but was more abundant in regions such as the septum, preoptic area, suprachiasmatic nucleus, lateral hypothalamic area, thalamus, pretectum and tegmentum. Less conspicuous was the innervation of the pallial regions, habenula, optic tectum, rhombencephalic reticular formation and spinal cord. Orexinergic innervation was found in close contact with dopaminergic, noradrenergic and serotoninergic cell groups, homologous to the substantia nigra in the midbrain tegmentum, the locus coeruleus, the nucleus of the solitary tract and the raphe nuclei. Although unique features have been found for lungfishes, the location of orexin immunoreactive elements is largely consistent with that recently reported following a similar approach in amphibians and amniotes, suggesting that the general organization of this peptidergic system occurred in the common ancestor of lungfishes and tetrapods.

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Section: Distribution Of Orexin-ir Fibers In the Brain Of Vertebratessupporting

confidence: 90%

Section: Orexin-ir Neurons In the Brain Of Vertebratesmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Possible Relationships Between the Orexinergic And Monoaminementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes Protopterus dolloi and Neoceratodus forsteri

et al. 2009

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Neuropeptide Y and orexin immunoreactivity in the sparrow brain coincide with seasonal changes in energy balance and steroids

Fokidis

Radin

et al. 2018

J of Comparative Neurology

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The transition between the breeding and nonbreeding states is often marked by a shift in energy balance. Despite this well-known shift in energy balance, little work has explored seasonal differences in the orexigenic neuropeptides that regulate food intake in wild animals. Here we tested the hypothesis that free-living male song sparrows (Melospiza melodia) show seasonal changes in energetic state, circulating steroids, and both neuropeptide Y (NPY) and orexin (OX) immunoreactivity. Nonbreeding song sparrows had more fat and muscle, as well as a ketone and triglyceride profile suggesting a greater reliance on lipid reserves. Breeding birds had higher plasma androgens; however, nonbreeding birds did maintain androgen precursors in circulation. Nonbreeding birds had more NPY immunoreactivity, specifically in three brain regions: lateral septum, bed nucleus of the stria terminalis, and ventral tegmental area. Furthermore, nonbreeding birds had more OX immunoreactivity in multiple brain regions. Taken together, the data indicate that a natural shift in energy balance is associated with changes in NPY and OX in a region-specific manner.

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Opposing effects of hypoxia on catecholaminergic locus coeruleus and hypocretin/orexin neurons in chick embryos

Landry

Hawkins

Wiebe

et al. 2014

Developmental Neurobiology

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Terrestrial vertebrate embryos face a risk of low oxygen availability (hypoxia) that is especially great during their transition to air-breathing. To better understand how fetal brains respond to hypoxia, we examined the effects of low oxygen availability on brain activity in late-stage chick embryos (day 18 out of a 21-day incubation period). Using cFos protein expression as a marker for neuronal activity, we focused on two specific, immunohistochemically identified cell groups known to play an important role in regulating adult brain states (sleep and waking): the noradrenergic neurons of the Locus Coeruleus (NA-LC), and the Hypocretin/Orexin (H/O) neurons of the hypothalamus. cFos expression was also examined in the Pallium (the avian analog of the cerebral cortex). In adult mammalian brains, cFos expression changes in a coordinated way in these areas. In chick embryos, oxygen deprivation simultaneously activated NA-LC while deactivating H/O-producing neurons; it also increased cFos expression in the Pallium. Activity in one pallial primary sensory area was significantly related to NA-LC activity. These data reveal that at least some of the same neural systems involved in brain-state control in adults may play a central role in orchestrating prenatal hypoxic responses, and that these circuits may show different patterns of coordination than seen in adults.

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Distribution of orexin/hypocretin immunoreactivity in the brain of a male songbird, the house finch, Carpodacus mexicanus

Cited by 8 publications

References 50 publications

Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes <i>Protopterus dolloi</i> and <i>Neoceratodus forsteri</i>

Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes <i>Protopterus dolloi</i> and <i>Neoceratodus forsteri</i>

Neuropeptide Y and orexin immunoreactivity in the sparrow brain coincide with seasonal changes in energy balance and steroids

Opposing effects of hypoxia on catecholaminergic locus coeruleus and hypocretin/orexin neurons in chick embryos

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