Distribution of non-tuberculosis mycobacteria in environmental samples from a slaughterhouse and in raw and processed meats

Shitaye, J.E.; Horvathova, A.; Bartosova, L.; Morávková, Monika; Kaevska, Marija; Donnelly, Neysan; Pavlík, I.

doi:10.17221/120/2008-cjfs

Cited by 11 publications

(4 citation statements)

References 32 publications

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“…In Brazil, NTM was found in 9 to 25% of raw milk samples and related dairy products (Franco et al, 2013). Similar levels were obtained from analyses of raw and processed meat samples from the Czech Republic (Shitaye et al, 2009). High levels of NTM contamination were also reported in raw milk samples analyzed in several countries from Europe and Africa (Kazwala et al, 1998;Konuk et al, 2007).…”

Section: Introductionsupporting

confidence: 64%

“…Previously, NTM have been detected in meat and cow milk (raw and pasteurized; Sgarioni et al, 2014), major food sources for humans. Water, soil, and food have been suggested as important NTM sources for infection transmission to humans (Shitaye et al, 2009;Sgarioni et al, 2014). The relationship between environmental sources of NTM and disease in humans has been studied for decades (Wolinsky, 1979), but mostly as association studies because of a lack of data on the genetic basis of the relationship.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic analysis of nontuberculous mycobacteria isolated from raw milk and human cases in Wisconsin

Ali

Hanafy

Hansen

et al. 2021

Journal of Dairy Science

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Nontuberculous mycobacteria (NTM) compose a group of mycobacteria that do not belong to the Mycobacterium tuberculosis complex group. They are frequently isolated from environmental samples such as water, soil, and, to a lesser extent, food samples. Isolates of NTM represent a major health threat to humans worldwide, especially those who have asthma or are immunocompromised. Human disease is acquired from environmental exposures and through consumption of NTM-contaminated food. The most common clinical manifestation of NTM disease in human is lung disease, but lymphatic, skin and soft tissue, and disseminated disease are also important. The main objective of the current study was to profile the farm-level contamination of cow milk with NTM by examining milk filters and bulk tank milk samples. Five different NTM species were isolated in one dairy herd in Wisconsin, with confirmed 16S rRNA genotypes including Mycobacterium fortuitum, Mycobacterium avium ssp. hominissuis, Mycobacterium abscessus, Mycobacterium simiae, and Mycobacterium avium ssp. paratuberculosis (Mycobacterium paratuberculosis). In tank milk samples, M. fortuitum was the predominant species in 48% of the samples, whereas M. chelonae/abscessus and M. fortuitum were the only 2 species obtained from 77 and 23% of the examined filters, respectively. Surprisingly, M. avium ssp. hominissuis, M. paratuberculosis, and M. simiae were isolated from 16.7, 10.4, and 4% of the examined milk samples, respectively, but not from milk filters. Interestingly, NTM isolates from human clinical cases in Wisconsin clustered very closely with those from milk samples. These findings suggest that the problem of NTM contamination is underestimated in dairy herds and could contribute to human infections with NTM. Overall, the study validates the use of bulk tank samples rather than milk filters to assess contamination of milk with NTM. Nontuberculous mycobacteria represent one type of pathogens that extensively contaminate raw milk at the farm level. The significance of our research is in evaluating the existence of NTM at the farm level and identifying a simple approach to examine the potential milk contamination with NTM members using tank milk or milk filters from dairy operations. In addition, we attempted to examine the potential link between NTM isolates found in the farm to those circulating in humans in Wisconsin.

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Section: Introductionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Genotypic analysis of nontuberculous mycobacteria isolated from raw milk and human cases in Wisconsin

Ali

Hanafy

Hansen

et al. 2021

Journal of Dairy Science

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“…Non‐tuberculous mycobacteria have been isolated ubiquitously in nature, that is, in the environment: in soil (Covert et al., ; Martin‐Casabona et al., ) and water (Collins et al., ; Shitaye et al., ), as well as in animals and humans (Kazwala et al., ; Berg et al., ). Some NTM species have occasionally been isolated from animals in the Kruger National Park and the Hluhluwe–iMfolozi Park in South Africa (Michel et al., ; Michel et al.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Distribution of Non-Tuberculous Mycobacteria (NTM) in Cattle, African Buffaloes (Syncerus caffer) and their Environments in South Africa

Gcebe

Rutten

Pittius

et al. 2013

Transbound Emerg Dis

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SummaryIt has been hypothesized that a variety of non-tuberculous mycobacteria (NTM) species to which livestock and wildlife species are naturally exposed induce broadly cross-reactive anti-mycobacterial immune responses which interfere with current standard diagnostic assays. Non-tuberculous mycobacteria have also been implicated in Mycobacterium bovis-specific immune responsiveness, hence potentially the development of tuberculosis. Cattle and African buffaloes are both maintenance hosts of bovine tuberculosis (BTB) in South Africa, yet the effective diagnosis and control in these species may be hampered by adverse effects of NTM. As part of an investigation of the role of NTM in the immune responsiveness of cattle and African buffaloes to NTM, we conducted a countrywide survey to establish the prevalent NTM species and their distribution in the natural environments of these animals. A total of 1123 samples (water, soil, nasal and pharyngeal swabs) were collected for mycobacterium isolation. In addition, NTM isolated from tissue samples between 1991 and 2011 were included in the analysis. Mycobacteria were isolated from 56% of the samples from the countrywide survey. A total of 420 NTM isolates from soil, water, animal tissues and animalderived swab samples were genotyped with the following results: 302 belonged to 40 known NTM species, 79 were found to be closely related to 23 known NTM species, and 38 isolates were found to be potentially novel species that are not currently listed in the RIDOM and NCBI BLAST databases. The four NTM species or closely related groups most frequently isolated in this survey included Mycobacterium terrae (11.2% of isolates), a group of mycobacteria closely related to Mycobacterium moriokaense (referred to as M. moriokaense-like) (8.1% of isolates), Mycobacterium nonchromogenicum (7.4% of isolates) and Mycobacterium vaccae/M. vanbaalenii (5.2% of isolates). The phylogenetic analysis of the M. moriokaense-like isolates, based on the 16S rRNA sequences, revealed at least eight clusters, possibly associated with eight different NTM species. Our findings provide account of NTM species diversity and associated prevalences in cattle and African buffaloes and their environments in South Africa. The identification of the most prevalent NTM species in this study will allow for a targeted investigation of their effects on host immune responses.

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“…Approximately two grams of tissue samples in two ml of NSS were used to prepare homogenates. The tissue samples were de‐contaminated as per Shitaye's method (Shitaye et al., ).…”

Section: Methodsmentioning

confidence: 99%

Identification of Mycobacterium species from apparently healthy freshwater aquarium fish using partial sequencing and PCR-RFLP analysis of heat shock protein (hsp65 ) gene

Shukla

Sharma

et al. 2014

J. Appl. Ichthyol.

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The present study analysed the incidence of mycobacteria in apparently healthy looking freshwater aquarium fish in Uttar Pradesh (State), India. Sixty fish belonging to eight different species were collected from six aquarium shops in different cities and processed for isolation of Mycobacterium species. Using the initial protocol of decontamination of tissue homogenates (with 1N HCl & 2N NaOH) and incubation at 30°C for 2 months, Mycobacterium sp. was isolated from 25% of the fish. The isolates were identified by standard biochemical tests. A 441 bp fragment of the hsp65 gene was amplified and digested by two fastdigest restriction enzymes, BstEII and HaeIII. Digested products were analysed using agarose gel electrophoresis. Sequencing of amplified fragments of the hsp65 gene was also performed. Isolates were identified as: five isolates of M. abscessus, three M. gordonae, two M. fortuitum, two M. conceptionense, two M. parascrofulaceum, and one isolate of M. senegalense. Mycobacterial incidence in apparently healthy looking freshwater aquarium fish is dreadful and the study is relevant because of the mycobacterial diversity related to aquarium fish and its zoonotic importance. All Mycobacterium species isolated in this study are well known pathogens in humans as well as fish.U.S.

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Distribution of non-tuberculosis mycobacteria in environmental samples from a slaughterhouse and in raw and processed meats

Cited by 11 publications

References 32 publications

Genotypic analysis of nontuberculous mycobacteria isolated from raw milk and human cases in Wisconsin

Genotypic analysis of nontuberculous mycobacteria isolated from raw milk and human cases in Wisconsin

Prevalence and Distribution of Non-Tuberculous Mycobacteria (NTM) in Cattle, African Buffaloes (Syncerus caffer) and their Environments in South Africa

Identification of Mycobacterium species from apparently healthy freshwater aquarium fish using partial sequencing and PCR-RFLP analysis of heat shock protein (hsp65 ) gene

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