Distribution of nitric oxide synthase-immunoreactive nerves and identification of the cellular targets of nitric oxide in guinea-pig and human urinary bladder by cGMP immunohistochemistry

Smet, Peter J.; Jonavicius, Jarmila; Marshall, Villis R.; Vente, Jan de

doi:10.1016/0306-4522(95)00453-x

Cited by 257 publications

(275 citation statements)

References 39 publications

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“…It is hypothesised that these cells modulate and co-ordinate activity of detrusor bundles facilitating the large, slower overactive-bladder contractions (Hashitani et al, 2004). Interstitial cells may also be a control point for regulation of spontaneous activity; they are innervated by afferent nerves labelling for nitric oxide synthase (Smet et al, 1996), and they also express cGMP activity. Our understanding of these cells is in its infancy, but they potentially form an important target to modulate overactive bladder activity.…”

Section: Figure 4 Near Herementioning

confidence: 99%

The physiological function of lower urinary tract smooth muscle

Fry

Meng

Young

2010

Autonomic Neuroscience

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Section: Figure 4 Near Herementioning

confidence: 99%

The physiological function of lower urinary tract smooth muscle

Fry

Meng

Young

2010

Autonomic Neuroscience

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“…Following stimulation with sodium nitroprusside, interstitial Sexual and LUT function AF Brading et al cells throughout the bladder demonstrated an intense induction of cGMP immunoreactivity, but detrusor muscle cells remained uniformly negative. 47 Therefore, interstitial cells in the bladder may be involved in the neuromuscular transmission, and again changes in interstitial cells may account for increased excitability of detrusor smooth muscle in overactive bladders.…”

Section: Possible Role Of Interstitial Cells In Lutsmentioning

confidence: 99%

“…Myofibroblast form very close association with suburothelial afferents and may also be the target of efferent nitrergic nerves. 47 Isolated cells are spontaneously active, and furthermore application of ATP initiates inward currents and corresponding transient increases of intracellular [Ca 2 þ ] through the activation of P2Y receptors. 37 These cells are ideally located to play a modulatory role in the process of bladder sensation and may play an intermediate and variable gain stage in the process of bladder fullness sensation.…”

Section: Possible Role Of Interstitial Cells In Lutsmentioning

confidence: 99%

“…64 In response to NO donors, the smooth muscle does not react by generating cGMP, although the interstitial cells in the bladder do so. 47 It has been suggested that it is these cells that are targeted by the nitrergic nerves, although the functional significance is unknown.…”

Section: Sexual and Lut Function Af Brading Et Almentioning

confidence: 99%

“…In a series of studies, Smet and co-workers have investigated the immunohistochemistry of the ganglia found in the human bladder wall, and the nerves innervating them. 47,95 The ganglia themselves were found in the lateral walls of the bladder and contained between 1 and 36 neurons. Most of the cell bodies were immunoreactive to various peptides (VIP, NPY, galanin) and thought to be postganglionic parasympathetic neurons, but the occasional neuron showed immunoreactivity to tyrosine hydroxylase, suggesting it to be a postganglionic sympathetic neuron.…”

Section: Autonomic Innervation Of the Pelvismentioning

confidence: 99%

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A survey of commonalities relevant to function and dysfunction in pelvic and sexual organs

2007

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Micturition, defecation and sexual function are all programmed through spinal reflexes that are under descending control from higher centres. Interaction between these reflexes can clearly be perceived, and evidence is accumulating the dysfunction in one reflex is often associated with dysfunction in another. In this article, we describe some of the basic properties and neural control of the smooth muscles mediating the reflexes, reviewing the common features that underlie these reflex functions, and what changes may be responsible for dysfunction. We propose that autonomic control within the pelvis predisposes pelvic and sexual organs to crosstalk, with the consequence that diseases and conditions of the pelvis are subject to convergence on a functional level. It should be expected that disturbance of the function of one system will inevitably impact adjacent systems.

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Colocalization of nitric oxide synthase and some neurotransmitters in the intramural ganglia of the guinea pig urinary bladder

Zhou

Ling

1998

J. Comp. Neurol.

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The distribution of nitrergic neurons was investigated by using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and nitric oxide synthase (NOS) immunohistochemistry in wholemount preparations of the urinary bladder in guinea pigs. Both NADPH-d+ and NOS+ neurons were located predominantly in the bladder base. Double staining showed that 70.9% of the NADPH-d+ neurons coexpressed NOS. Acetylcholinesterase histochemistry revealed that a majority of the intramural neurons were reactive, and about half of them (51.4%) were double labelled for NOS. Tyrosine hydroxylase-positive neurons were also distributed mainly in the bladder base but in a neuronal population that was separate from the preponderant NADPH-d+ neurons. Vasoactive intestinal polypeptide immunoreactivity was also detected in the some of intramural ganglion cells, in which 21.3% of them coexpressed NADPH-d. Calcitonin gene-related peptide and substance P immunoreactivities were confined to nerve fibers, often in close association with NADPH-d+ cells or extended along the blood vessels. These results have demonstrated the colocalization of NADPH-d and NOS in the majority of intramural ganglion cells. Many of the nitrergic neurons are apparently cholinergic, indicating that they are parasympathetic postganglionic neurons, and this underscores NO as the major neuromodulator in the parasympathetic nerves in the bladder walls. The localization of vasoactive intestinal polypeptide in nitrergic neurons suggests that the peptide may complement NO for regulation of micturition reflex. The close relationship of NADPH-d-reactive intramural neurons with calcitonin gene-related peptide and substance P fibers, most probably derived from dorsal root ganglion cells, suggests that NO released from the local neurons may exert its influence on the sensory neural pathways in the urinary bladder.

show abstract

Distribution of nitric oxide synthase-immunoreactive nerves and identification of the cellular targets of nitric oxide in guinea-pig and human urinary bladder by cGMP immunohistochemistry

Cited by 257 publications

References 39 publications

The physiological function of lower urinary tract smooth muscle

The physiological function of lower urinary tract smooth muscle

A survey of commonalities relevant to function and dysfunction in pelvic and sexual organs

Colocalization of nitric oxide synthase and some neurotransmitters in the intramural ganglia of the guinea pig urinary bladder

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