Distribution of NGF and NT‐3‐like protein immunoreactivity in the teleost kidney

Arcamone, N.; Lucini, Carla; Borzacchiello, Giuseppe; Castaldo, L.; Gargiulo, Giuseppe; Girolamo, Paolo de

doi:10.1002/jemt.20138

Cited by 8 publications

(12 citation statements)

References 45 publications

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“…We detected by Western blotting in head homogenates molecules with equivalent molecular weights to the mammalian BDNF and NT‐3, which agrees with previous results in other teleostean species (Lucini et al. 2003; Arcamone et al. 2005).…”

Section: Discussionsupporting

confidence: 91%

Neurotrophin and Trk neurotrophin receptors in the inner ear of Salmo salar and Salmo trutta

et al. 2006

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Neurotrophins (NTs) and their signal transducing Trk receptors play a critical role in the development and maintenance of specific neuronal populations in the nervous system of higher vertebrates. They are responsible for the innervation of the inner ear cochlear and vestibular sensory epithelia. Neurotrophins and Trks are also present in teleosts but their distribution in the inner ear is unknown. Thus, in the present study, we used Western-blot analysis and immunohistochemistry to investigate the expression and cell localization of both NTs and Trk receptors in the inner ear of alevins of Salmo salar and Salmo trutta . Western-blot analysis revealed the occurrence of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), but not nerve growth factor (NGF), as well as all three Trk receptors, i.e. TrkA, TrkB and TrkC, the estimated molecular weights of which were similar to those expected for mammals. Specific immunoreactivity for neurotrophins was detected mainly in the sensory epithelia. In particular, BDNF immunoreactivity was found in the maculae of the utricle and saccule, whereas NT-3 immunoreactivity was present in the sensory epithelium of the cristae ampullaris. As a rule the sensory epithelia of the inner ear lacked immunoreactivity for Trks, thus excluding possible mechanisms of autocrinia and/or paracrinia. By contrast, overlapping subpopulations of neurons in the statoacoustic ganglion expressed TrkA (about 15%), TrkB (about 65%) and TrkC (about 45%). The present results demonstrate that, as in mammals and birds, the inner ear of teleosts expresses the components of the neurotrophin-Trk system, but their roles remain to be elucidated.

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Section: Discussionsupporting

confidence: 91%

Neurotrophin and Trk neurotrophin receptors in the inner ear of Salmo salar and Salmo trutta

et al. 2006

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“…NT‐3 antiserum identified a band presumably corresponding to proNT‐3. In previous studies on different teleostean species, the same antibody has revealed bands corresponding to mature form in different tissues (Lucini et al, ; Arcamone et al, ; Catania et al, ). It is likely that in zebrafish brain proNT‐3 occurs and elicits its functions as in mammals (Negro et al, ; Krause et al, ).…”

Section: Discussionmentioning

confidence: 90%

“…In zebrafish brain homogenates, NGF antiserum recognized a band likely corresponding to proNGF protein, as well as in the brain of the teleost N. furzeri (D'Angelo et al, 2014) and in mammalian brain (Fahnestock et al, ), and differently from gut and kidney of other teleosts (Lucini et al, ; Arcamone et al, ). BDNF antibody specifically recognized two bands corresponding presumably to pro and mature BDNF, with slight differences with the results reported in the retina and lateral line system of zebrafish during development (Germanà et al, ,), in the brain of N. furzeri (D'Angelo et al, 2014) and in C. dimerus (Vissio et al, ).…”

Section: Discussionmentioning

confidence: 99%

Neurotrophins and their Trk‐receptors in the cerebellum of zebrafish

Gatta

Altamura

Avallone

et al. 2016

Journal of Morphology

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Neurotrophins (NTs) and their specific Trk-receptors are key molecules involved in the regulation of survival, proliferation, and differentiation of central nervous system during development and adulthood in vertebrates. In the present survey, we studied the expression and localization of neurotrophins and their Trk-receptors in the cerebellum of teleost fish Danio rerio (zebrafish). Teleostean cerebellum is composed of a valvula, body and vestibulolateral lobe. Valvula and body show the same three-layer structure as cerebellar cortex in mammals. The expression of NTs and Trk-receptors in the whole brain of zebrafish has been studied by Western blotting analysis. By immunohistochemistry, the localization of NTs has been observed mainly in Purkinje cells; TrkA and TrkB-receptors in cells and fibers of granular and molecular layers. TrkC was faintly detected. The occurrence of NTs and Trk-receptors suggests that they could have a synergistic action in the cerebellum of zebrafish. J. Morphol. 277:725-736, 2016. © 2016 Wiley Periodicals, Inc.

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“…Accordingly, the presence of pro‐NGF was also reported in the brain of the teleost Nothobranchius furzeri , by employing the same antiserum (D'Angelo et al., ,b). On the other hand, this antiserum detected the mature form of NGF in different organs of fish, such as gut (Lucini et al., ) and kidney (Arcamone et al., ). Taking into consideration that the employed NGF antiserum is able to detect both pro‐ and mature NGF in fish tissues, our results suggest that that only the pro‐NGF form is present in the brain of zebrafish.…”

Section: Discussionmentioning

confidence: 99%

Nerve growth factor is expressed and stored in central neurons of adult zebrafish

et al. 2019

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Nerve growth factor (NGF), a member of the neurotrophin family, was initially described as neuronal survival and growth factor, but successively has emerged as an active mediator in many essential functions in the central nervous system of mammals. NGF is synthesized as a precursor pro-NGF and is cleaved intracellularly into mature NGF. However, recent evidence demonstrates that pro-NGF is not a simple inactive precursor, but is also secreted outside the cells and can exert multiple roles. Despite the vast literature present in mammals, studies devoted to NGF in the brain of other vertebrate models are scarce. Zebrafish is a teleost fish widely known for developmental genetic studies and is well established as model for translational neuroscience research. Genomic organization of zebrafish and mouse NGF is highly similar, and zebrafish NGF protein has been reported in mature and two-precursors forms. To add further knowledge on neurotrophic factors in vertebrate brain models, we decided to determine the NGF mRNA and protein distribution in the adult zebrafish brain and to characterize the phenotype of NGF-positive cells. NGF mRNA was visualized by in situ hybridization on wholemount brains. NGF protein distribution was assessed on microtomic sections by using an antiserum against NGF, able to recognize pro-NGF in adult zebrafish brain as demonstrated also in previous studies. To characterize NGF-positive cells, anti-NGF was employed on microtomic slides of aromatase B transgenic zebrafish (where radial glial cells appeared fluorescent) and by means of double-immunolabeling against NGF/proliferative cell nuclear antigen (PCNA; proliferation marker) and NGF/microtube-associated protein2 (MAP2; neuronal marker). NGF mRNA and protein were widely distributed in the brain of adult zebrafish, and their pattern of distribution of positive perikaryal was overlapping, both in males and females, with few slight differences. Specifically, the immunoreactivity to the protein was observed in fibers over the entire encephalon. MAP2 immunoreactivity was present in the majority of NGF-positive cells, throughout the zebrafish brain. PCNA and aromatase B cells were not positive to NGF, but they were closely intermingled with NGF cells. In conclusion, our study demonstrated that mature neurons in the zebrafish brain express NGF mRNA and store pro-NGF.

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Distribution of NGF and NT‐3‐like protein immunoreactivity in the teleost kidney

Cited by 8 publications

References 45 publications

Neurotrophin and Trk neurotrophin receptors in the inner ear of Salmo salar and Salmo trutta

Neurotrophin and Trk neurotrophin receptors in the inner ear of Salmo salar and Salmo trutta

Neurotrophins and their Trk‐receptors in the cerebellum of zebrafish

Nerve growth factor is expressed and stored in central neurons of adult zebrafish

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