Distribution of Mycobacterium leprae Strains among Cases in a Rural and Urban Population of Maharashtra, India

Kuruwa, Sanjana; Vissa, Varalakshmi; Mistry, Nerges

doi:10.1128/jcm.05315-11

Cited by 17 publications

(11 citation statements)

References 19 publications

(24 reference statements)

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“…On the whole, our study showed a distribution of M. leprae with the 1D SNP subtype being dominant, followed by 1C, 1A and 2G from India, which is similar to that reported from Nepal and Bangladesh [19]. One of the previous studies from Western India in a rural and urban area near Mumbai reported mainly subtype D, followed by B [29].…”

Section: Discussionsupporting

confidence: 88%

Single nucleotide polymorphisms typing of Mycobacterium leprae reveals focal transmission of leprosy in high endemic regions of India

Lavania¹,

Jadhav²,

Turankar

et al. 2013

Clinical Microbiology and Infection

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Earlier studies indicate that genotyping of Mycobaterium leprae based on single-nucleotide polymorphisms (SNPs) is useful for analysis of the global spread of leprosy. In the present study, we investigated the diversity of M. leprae at eight SNP loci using 180 clinical isolates obtained from patients with leprosy residing mainly in Delhi and Purulia (West Bengal) regions. It was observed that the frequency of SNP type 1 and subtype D was most predominant in the Indian population. Further, the SNP type 2 subtype E was noted only from East Delhi region and SNP type 2 subtype G was noted only from the nearby areas of Hoogly district of West Bengal. These results indicate the occurrence of focal transmission of M. leprae infection and demonstrate that analysis by SNP typing has great potential to help researchers in understanding the transmission of M. leprae infection in the community.

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Section: Discussionsupporting

confidence: 88%

Single nucleotide polymorphisms typing of Mycobacterium leprae reveals focal transmission of leprosy in high endemic regions of India

Lavania¹,

Jadhav²,

Turankar

et al. 2013

Clinical Microbiology and Infection

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“…In 2009 itself, Cebu, Philippines leprosy patients have strain typed and predominant copy number 8,9,5,15,3,9,14,9,5,7,21,24, . Like South India leprosy patients, North India and Mumbai leprosy patients were also strain typed and these results were somewhat resembles with the South Indian leprosy patients [37,38].…”

Section: Reviewmentioning

confidence: 58%

Variable Number Tandem Repeats for Global Strain Typing and Strain Differentiation of Mycobacterium leprae

Chokkakula¹,

Abdelrawaf²,

Attitalla³

et al. 2014

J Med Microb Diagn

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Leprosy is chronic granulomatous disease caused by Mycobacterium leprae and associated with the disability, stigma and discrimination to the affected individuals. Though often considered a disease of antiquity, it is found most commonly today in tropical and sub-tropical regions. Global efforts to eradicate leprosy have been largely successful in controlling its spread. Despite these efforts, the disease remains endemic countries emphasizing the need for greater scrutiny of its epidemiology. Strain typing and strain differentiation by Variable Number Tandem repeats (VNTR) could be useful in tracing origins and routes of infection, general leprosy surveillance and prevalence. Strain typing of Mycobacterium leprae by VNTR has been successfully carried out and predominance of leprosy bacilli in different geographical region also done by VNTR. In the present review, we reported global distribution M. leprae by Variable Number tandem repeats in systematic way to easy understanding of leprosy distribution. ReviewLeprosy is chronic granulomatous disease caused by Mycobacterium leprae and associated with the disability, stigma and discrimination to the affected individuals [1]. Though often considered a disease of antiquity, it is found most commonly today in tropical and sub-tropical regions [2]. Global efforts to eradicate leprosy have been largely successful in controlling its spread. Despite these efforts, the disease remains endemic countries, with 232,857 cases reported cases globally in 2012 [3]. India accounted for 1.27 of cases reported in 2011-2012, emphasizing the need for greater scrutiny of its epidemiology. strain typing and strain differentiation are very helpful to identify the source of infection, transmission of infection and spreading of disease, differentiating cases of relapse from re infection, and for unravelling possible links between human and nonreservoir sources [4,5]. In recent years molecular typing methodologies have complemented conventional infectious disease.Strain tying and strain differentiation is a method which useful to distinguishing members of the same microbial species from one another on the basis of genotype. Strain typing explains that one isolate is same or it's different. Several molecular typing methods have been employed to distinguish the M. leprae strains over the years.Initially methods like surface antigen typing [6], multi-locus enzyme electrophoresis (MLEE) [7] and phage typing [8] were used for strain typing. DNA based strain typing methods were started from 1990. The methods includes fragment length polymorphism (RFLP) typing at locus such as rRNA operons [9] fingerprinting by randomly primed PCR [10] pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST) [11], variable umber tandem repeats (VNTR) and SNP. If genetic diversity less, VNTR is good source for the identification of strain variation [12,13]. VNTRs have short, tandemly repeated motifs typically ranging from 1 to 60 bp in length. Polymorphisms at those sites consist of differe...

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“…Analysis of allele frequencies of these VNTRs from published data from North India (Lavania et al, 2011), South India (Shinde et al, 2009) and Maharashtra (Kuruwa et al, 2012), suggested a number of demographic associations among strains of M. leprae. Strains from Maharashtra and South India have an identical predominant allele except (AC)8a and 6-7 with the 8 and 6 allele being predominant respectively in our strains.…”

Section: Discussionmentioning

confidence: 96%

“…Each sample is represented as having a distinct profile which may be attributed to polymorphic VNTR loci. Fontes et al, 2009Fontes et al, , 2012Sakamuri et al, 2009;Shinde et al, 2009, Kuruwa et al, 2012. Our study has been carried out with the aim of analysing various known genomic markers to elicit epidemiologically relevant information about the transmission of the disease and to compare strain types within the studied population with VNTRs.…”

Section: Discussionmentioning

confidence: 98%

Genotyping of Mycobacterium leprae strains from a region of high endemic leprosy prevalence in India

Lavania¹,

Jadhav²,

Turankar³

et al. 2015

Infection, Genetics and Evolution

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Distribution of Mycobacterium leprae Strains among Cases in a Rural and Urban Population of Maharashtra, India

Cited by 17 publications

References 19 publications

Single nucleotide polymorphisms typing of Mycobacterium leprae reveals focal transmission of leprosy in high endemic regions of India

Single nucleotide polymorphisms typing of Mycobacterium leprae reveals focal transmission of leprosy in high endemic regions of India

Variable Number Tandem Repeats for Global Strain Typing and Strain Differentiation of Mycobacterium leprae

Genotyping of Mycobacterium leprae strains from a region of high endemic leprosy prevalence in India

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