Distribution of Mosquitoes in the South East of Argentina and First Report on the Analysis Based on 18S rDNA and COI Sequences

Nieto, Leonardo Martín; Maciá, Arnaldo; Parisi, Gustavo; Farina, Juan L.; Vidal-Domínguez, María E.; Perotti, M. Alejandra; Berón, Corina M.

doi:10.1371/journal.pone.0075516

Cited by 11 publications

(7 citation statements)

References 45 publications

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“…5 B–D). Other studies, based on morphological characters, suggested that Aedeomyia, Uranotaenia and Toxorhynchites genera are ancient and basal groups in the Culicinae subfamily 62 – 65 suggesting that our phylogenetic reconstruction based on amino acid sequences may have recovered the true position of those genera. This is in line with the widespread knowledge that conserved amino acid sequences are more appropriate to recover deep branching patterns 21 , 66 , 67 , but due to the high branch support of a clade encompassing the three studied species from these genera in the nucleotide based trees (Fig.…”

Section: Discussionmentioning

confidence: 77%

Culicidae evolutionary history focusing on the Culicinae subfamily based on mitochondrial phylogenomics

Silva

Machado

Paula

et al. 2020

Sci Rep

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Mosquitoes are insects of medical importance due their role as vectors of different pathogens to humans. There is a lack of information about the evolutionary history and phylogenetic positioning of the majority of mosquito species. Here we characterized the mitogenomes of mosquito species through low-coverage whole genome sequencing and data mining. A total of 37 draft mitogenomes of different species were assembled from which 16 are newly-sequenced species. We datamined additional 49 mosquito mitogenomes, and together with our 37 mitogenomes, we reconstructed the evolutionary history of 86 species including representatives from 15 genera and 7 tribes. Our results showed that most of the species clustered in clades with other members of their own genus with exception of Aedes genus which was paraphyletic. We confirmed the monophyletic status of the Mansoniini tribe including both Coquillettidia and Mansonia genus. The Aedeomyiini and Uranotaeniini were consistently recovered as basal to other tribes in the subfamily Culicinae, although the exact relationships among these tribes differed between analyses. These results demonstrate that low-coverage sequencing is effective to recover mitogenomes, establish phylogenetic knowledge and hence generate basic fundamental information that will help in the understanding of the role of these species as pathogen vectors.

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Section: Discussionmentioning

confidence: 77%

Culicidae evolutionary history focusing on the Culicinae subfamily based on mitochondrial phylogenomics

Silva

Machado

Paula

et al. 2020

Sci Rep

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“…crinifer is restricted to South America (Arnell, ; WRBU, ), and sequences of Ae. crinifer from Argentina obtained by Díaz‐Nieto et al, () were analyzed and the average intraspecific distance between Ae. crinifer and our Ae.…”

Section: Discussionmentioning

confidence: 99%

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Chan-Chablé

Martínez‐Arce

Mis-Ávila³

et al. 2019

Ecology and Evolution

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Culicidae mosquitoes are potential vectors of pathogens that affect human health. The correct species identification, as well as the discovery and description of cryptic species, is important in public health for the control and management of specific vectors. In the present study, the diversity of anthropophagous mosquitoes in Quintana Roo, at the border between Mexico and Belize, was evaluated using morphological and molecular data (COI‐DNA Barcoding). A total of 1,413 adult female specimens were collected, belonging to eight genera and 31 morphospecies. Most species formed well‐supported clades. Intraspecific Kimura 2 parameters (K2P) distance average was 0.75%, and a maximum distance of 4.40% was observed for Anopheles crucians s.l. ABGD method identified 28 entities, while 32 entities were identified with the BIN system. In Culex interrogator and Culex nigripalpus a low interspecific genetic distance of 0.1% was observed. One undescribed species belonging to the genus Aedes ( Aedes n. sp.) was discovered, but no clear genetic divergence was found between this species and the closely related species Aedes angustivittatus . An intraspecific K2P distance greater than 2.7% was observed in Aedes serratus (3.9%), Anopheles crucians s.l. (4.4%), Culex taeniopus (3.7%), Haemagogus equinus (3.9%), Culex erraticus (5.0%), Psorophora ferox (4.5%), and in Anopheles apicimacula (8.10%); therefore, evidences of cryptic diversity are shown in these species. This study showed that DNA barcodes offer a reliable framework for mosquito species identification in Quintana Roo, except for some closely related species for which it is recommended to use additional nuclear genetic markers such as ITS2, in order to resolve these small discrepancies.

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“…Recently, Marrugal et al () concluded that phylogenetic analysis based on the ITS1 region is a useful tool to approach different taxonomic and phylogenetic issues in Ctenocephalides species, and they found clear molecular differences between C. felis and C. canis . In addition, the 18S rRNA gene has been widely used as a molecular marker in order to clarify molecular relationships among families and subfamilies (Whiting et al , ; Acosta & Morrone, ; Díaz‐Nieto et al , ).…”

Section: Introductionmentioning

confidence: 99%

Ctenocephalides felis and Ctenocephalides canis: introgressive hybridization?

Zurita

Callejón

Rojas

et al. 2016

Systematic Entomology

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In the present work, a comparative molecular study of Ctenocephalides felis and Ctenocephalides canis isolated from dogs (Canis lupus familiaris) from different geographical regions (Spain, Iran and South Africa) was carried out. We found morphological variations in C. felis that do not correspond with molecular differences. The Internal Transcribed Spacers 1 and 2 (ITS1, ITS2) and 18S rRNA partial gene, and cytochrome c-oxidase 1 (cox1) mtDNA partial gene sequences were determined to clarify the taxonomic status of these two species, and to assess interpopulation variation and interspecific sequence differences. In addition, a comparative phylogenetic study with other species of fleas using Bayesian, Maximum Parsimony and Maximum Likelihood analysis was performed. The 18S rRNA partial gene fragment was useful neither to discriminate C. canis and C. felis nor to infer phylogenetic relationships at this level, whereas ITS1 and ITS2 assessed for specific determination in the genus Ctenocephalides. The cox1 mtDNA sequences of C. felis revealed three main haplotypes and we suggest that there has been introgression of C. canis cox1 mtDNA into C. felis by Wolbachia pipientis. Based on cox1 sequences, restriction mapping identified many endonucleases that could be used to delineate different haplotypes of C. felis and to differentiate C. felis and C. canis.

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Distribution of Mosquitoes in the South East of Argentina and First Report on the Analysis Based on 18S rDNA and COI Sequences

Cited by 11 publications

References 45 publications

Culicidae evolutionary history focusing on the Culicinae subfamily based on mitochondrial phylogenomics

Culicidae evolutionary history focusing on the Culicinae subfamily based on mitochondrial phylogenomics

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Ctenocephalides felis and Ctenocephalides canis: introgressive hybridization?

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