Distribution of Morphological Variants of Calcium Deposits in Placenta With Iron-Deficiency Anemia of Gravidas

Popovych, A. I.; Давыденко, И. С.

doi:10.24061/1727-0847.15.3.2016.79

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“…In general, seven morphological variants of calcium deposits were studied in two localization areas 4 . The differences were observed in the chorial placental tree in the distribution frequency depending on the presence of IDAP for types II, III, IV of calcium deposits.…”

Section: Results Discussionmentioning

confidence: 99%

“…Calcification, liming or petrification (an increased level of insoluble calcium salts deposits) of the placenta is a fairly widespread phenomenon 1 , and placental calcification can often be found in combination with anemia in pregnancy 2,3 . Based on the previous studies 4,5 , it was found that the frequency of distribution of the studied variants of calcium deposits is different in iron deficiency anemia in pregnancy (IDAP) compared with the placental calcification without anemia (that is, with normal blood parameters during pregnancy). Moreover, these differences pertain to fibrinoid of the placenta, both in the area of the chorial tree as well as in the basal plate of the placenta.…”

Section: Introductionmentioning

confidence: 99%

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Histochemical Evaluation of Protein Oxidative Modification in Fibrinoid of the Placenta, Depending on Calcium Deposits, Combined With Iron Deficiency Anemia of Pregnancy

Popovych¹,

Давыденко²,

Davydenko³

2018

Arch Balk Med Union

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Evaluation histochimique des processus de modification de l'oxydation des protéines dans les fibroïdes du placenta dans sa calcification combinée avec l'anémie ferriprive des femmes enceintes Introduction. Le calcium et le fer affectent significativement le niveau des processus des radicaux libres. Le but de l'étude. Pour établir le niveau de modification oxydante des protéines dans le placenta fibroïde de localisation différente en fonction de la variante de dépôts de calcium sur la base de la technique histochimique avec du bleu de bromophénol aux protéines «aigres» et «basiques» par Mikel Calvo avec estimation quantitative des résultats de coloration par microspectrophotométrie. Matériaux et méthodes. Ont été étudiés 164 cas de calcinose placentaire, y compris 84 cas d'anémie ferriprive et 80 cas de grossesse non anémiques. On a réalisé une technique histochimique avec du bleu de bromophénol pour les protéines «aigres» et «basiques» par Mikel Calvo avec une estimation quantitative des ABSTRACT Introduction. Both calcium and iron significantly affect the level of free radical processes. The aim of the study was to determine the levels of protein oxidative modification in fibrinoid of placenta of different localization areas, depending on the variants of calcium deposits, based on histochemical methods of bromophenol blue staining of proteins into "acidic" and "basic" by Mikel Calvo with quantitative assessment of results by means of computed microspectrophotometry. Materials and methods. 164 cases of placental calcification, including 84 cases of iron deficiency anemia in pregnancy (IDAP) and 80 cases of pregnancy without anemia, were studied. Histochemical method of bromophenol blue into "acidic" and "basic" proteins by Mikel Calvo with the quantitative assessment of the staining results was performed by use of computed microspectrophotometry on the digital copy of the image with the fibrinoid areas in the computed program "ImageJ". Results. The differences between the average indicator R/B, which is the measure of protein oxidative

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Section: Results Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%