Distribution of microcrack surface density in the human otic capsule

Hansen, Lars Juul; Bloch, Sune Land; Frisch, Thomas; Sørensen, Mads Sølvsten

doi:10.1080/00016489.2021.1905875

Cited by 4 publications

(1 citation statement)

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“…In this study, voids were measured and quantified using an unbiased point grid to estimate the volume fraction of cellular void volume per capsular bone volume (19,20). A past study has shown that the volume of perilabyrinthine bone surrounding the semicircular canals is several times bigger than the volumes around the internal acoustic canal, cochlea, and window regions (21). Furthermore, the perilabyrinthine bone was subdivided into 200-μm-isowide concentric zones, in which the volume also increases with the circumference of the zone.…”

Section: Discussionmentioning

confidence: 99%

The Spatial Distribution of Cellular Voids in the Human Otic Capsule: An Unbiased Quantification of Osteocyte-Depleted Areas

2022

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Objective: This study aimed to describe the spatial distribution of osteocyte-depleted areas, so-called cellular voids, in the human otic capsule and compare it with that of otosclerosis. Background: Systematic histological studies of the bony otic capsule have revealed an osteoprotegerin (OPG)-mediated inhibition of normal bone remodeling around the inner ear. The resulting accumulation of bony degeneration and dead osteocytes has been thoroughly documented, and the spatial distribution of dead osteocytes and matrix microcracks resembles that of the human ear disease otosclerosis. Clusters of dead osteocytes that may interfere with osteocyte connectivity and thereby the OPG signaling pathway have been described in human temporal bones. It is possible that these cellular voids create disruptions in the antiresorptive OPG signal that may give rise to local pathological remodeling.Methods: Recently, a method of detecting cellular voids was developed. This study uses unbiased stereology to document the spatial distribution of cellular voids in bulk-stained undecalcified human temporal bone. Results: Cellular voids accumulate around the inner ear and increase in number and size with age. Furthermore, cellular voids are more frequently found in the anterior and lateral regions of the otic capsule, which are known predilection sites of otosclerosis. Conclusion: This colocalization of cellular voids and otosclerosis suggests a causal relationship between focal degeneration and otosclerotic remodeling.

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Section: Discussionmentioning

confidence: 99%