Distribution of maize mitochondrial transcripts in polysomal RNA: evidence for non-selectivity in recruitment of mRNAs

Aj, Yang; Rm, Mulligan

doi:10.1007/bf00312647

Cited by 14 publications

(7 citation statements)

References 15 publications

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“…Such mechanisms would be contrasted by analyses that detected a similar distribution of cox3 transcripts with different 5′-untranslated sequences in both polysomal fractions and total RNA prepared from maize mitochondria, implying that mitochondrial ribosomes non-preferentially associate with differently initiated transcripts (44). From our data on primary transcript 5′ termini in Arabidopsis mitochondria, we would favour a point of view that considers multiple promoters the result of a relaxed promoter specificity of the mitochondrial transcription machinery (34).…”

Section: Discussionmentioning

confidence: 99%

Multiple promoters are a common feature of mitochondrial genes in Arabidopsis

Kühn

Weihe

Börner

2005

Nucleic Acids Research

124

129

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Mitochondrial genes in the plant Arabidopsis thaliana are transcribed by two phage-type RNA polymerases encoded in the nucleus. Little is known about cis-elements that are recognized by these enzymes and mediate the transcription of the Arabidopsis mitochondrial genome. Here, 30 transcription initiation sites of 12 mitochondrial genes and gene clusters have been determined using 5′-RACE and ribonuclease protection analysis of primary transcripts labelled in vitro by guanylyltransferase. A total of 9 out of 12 genes were found to possess multiple promoters, revealing for the first time that multiple promoters are a common feature of mitochondrial genes in a dicotyledonous plant. No differences in promoter utilization were observed between leaves and flowers, suggesting that promoter multiplicity reflects a relaxed promoter specificity rather than a regulatory role of promoter selection. Nearly half the identified transcription initiation sites displayed immediately upstream a CRTA core sequence, which was mostly seen within the previously described CRTAAGAGA promoter motif or a novel CGTATATAA promoter element. About as many promoters possessed an ATTA or RGTA core. Our data indicate that the majority of mitochondrial promoters in Arabidopsis deviate significantly from the nonanucleotide consensus derived earlier for dicot mitochondrial promoters.

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Section: Discussionmentioning

confidence: 99%

Multiple promoters are a common feature of mitochondrial genes in Arabidopsis

Kühn

Weihe

Börner

2005

Nucleic Acids Research

124

129

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“…These sequence blocks are not present upstream of other genes, suggesting that they may be involved in binding gene-specific proteins involved in regulating translation. The presence of introns apparently has little influence on an mRNA being incorporated into the ribosome, as suggested by a study of cox2 transcripts in polysomes versus total mitochondrial RNA [71]. A similar distribution of intron-containing mRNAs and processed mRNAs for this gene was found in maize mitochondria in both polysomal and total RNA, suggesting that intron-containing RNA is just as competent for ribosome entry as is the processed mRNA.…”

Section: Ribosomal Regulationmentioning

confidence: 91%

Regulation of gene expression in plant mitochondria

1996

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Many genes is plant mitochondria have been analyzed in the past 15 years and regulatory processes controlling gene expression can now be investigated. In vitro systems capable of initiating transcription faithfully at promoter sites have been developed for both monocot and dicot plants and will allow the identification of the interacting nucleic acid elements and proteins which specify and guide transcriptional activities. Mitochondrial activity, although required in all plant tissues, is capable of adapting to specific requirements by regulated gene expression. Investigation of the factors governing the quality and quantity of distinct RNAs will define the extent of interorganelle regulatory interference in mitochondrial gene expression.

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“…Both edited and partially edited transcripts appear to be represented in association with polysomes (57,106). Moreover, little discrimination by the ribosome occurs with regard to untranslated leader sequences or transcript splicing status (184).…”

Section: Translational/posttranslational Regulationmentioning

confidence: 99%

Nuclear Control of Plastid and Mitochondrial Development in Higher Plants

León¹,

Arroyo²,

Mackenzie

1998

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

158

128

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The nucleus must coordinate organelle biogenesis and function on a cell and tissue-specific basis throughout plant development. The vast majority of plastid and mitochondrial proteins and components involved in organelle biogenesis are encoded by nuclear genes. Molecular characterization of nuclear mutants has illuminated chloroplast development and function. Fewer mutants exist that affect mitochondria, but molecular and biochemical approaches have contributed to a greater understanding of this organelle. Similarities between organelles and prokaryotic regulatory molecules have been found, supporting the prokaryotic origin of chloroplasts and mitochondria. A striking characteristic for both mitochondria and chloroplast is that most regulation is posttranscriptional.

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Distribution of maize mitochondrial transcripts in polysomal RNA: evidence for non-selectivity in recruitment of mRNAs

Cited by 14 publications

References 15 publications

Multiple promoters are a common feature of mitochondrial genes in Arabidopsis

Multiple promoters are a common feature of mitochondrial genes in Arabidopsis

Regulation of gene expression in plant mitochondria

Nuclear Control of Plastid and Mitochondrial Development in Higher Plants

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