Distribution of keratins, vimentin, and actin in the testis of two South American camelids: Vicuna(Vicugna vicugna)and llama(Lama glama). An immunohistochemical study

Rodríguez, A.; Rojas, Mariana; Bustos‐Obregón, Eduardo; Urquieta, Bessie; Regadera, Javier

doi:10.1002/(sici)1097-0185(19990301)254:3<330::aid-ar3>3.0.co;2-m

Cited by 17 publications

(16 citation statements)

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“…O u r f i n d i n g o f S e r t o l i c e l l p e r i n u c l e a r localization of the vimentin is consistent with previous reports in the rhesus monkey [25], rat [9,11,26], human [11,27,28], South American camelids [29], sheep [5], and bull [15]. Our observations of vimentin distribution from day-0 to 5 months of age and in mature bulls are in agreement with the previous studies in the bovine testis [10,15].…”

Section: Discussionsupporting

confidence: 93%

Postnatal Developmental Changes in Immunohistochemical Localization of .ALPHA.-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testes

Devkota

Sasaki

Takahashi³

et al. 2006

Journal of Reproduction and Development

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Abstract. The present study demonstrates the postnatal developmental changes in immunohistochemical localization of α-smooth muscle actin (SMA) and vimentin in the bovine testis. In the peritubular myoid cells of seminiferous tubules and the sub-epithelial and stromal cells of straight tubules and the rete testis, α-SMA starts appearing at around 4 months of age. Peritubular α-SMA attains the continuous mature pattern at around 5 months of age whereas sub-epithelial and stromal α-SMA increases with advancing age. Vimentin is localized in the perinuclear zone of Sertoli cells, peritubular and vascular wall cells, a few interstitial cells, and in the basal part of the epithelia of straight and rete tubules. Developmental changes are only evident in the Sertoli cell vimentin, which is basal and weak at birth and increases moderately until 4 months of age. From around 5 to 8 months of age when the Sertoli cells are under morphological transformation, vimentin intensity is considerably increased and the characteristic vimentin extensions connect the Sertoli nuclei to the basal membrane. These extensions get shorter at around 9 month of age as the Sertoli nuclei are positioned basally. The mature Sertoli cell perinuclear vimentin is strong and stable without infranuclear extension. In conclusion, the age of appearance of α-SMA coincides with the onset of postnatal division of spermatogonia, and vimentin may play a key role in stabilizing Sertoli cell nuclei during their transformation in bovine. positioning of intracellular organelles, forming of cytoplasmic extensions, and anchoring of organelles to the plasma membrane [1]. As a specific marker of smooth muscle differentiation [2], seminiferous peritubular α-smooth muscle actin (SMA) has been detected in the first few postnatal days in the rat testis [3,4] and in the early postnatal ovine testis [5]. Increasing patterns of α-SMA in the ovine testis [5] and changes in the actin filament arrangement in the rat testis [6] during p o s t n a t a l d e v e l o p m e n t h a v e s u g g e s t e d a relationship for SMA with testicular development and functions. However, no postnatal localization patterns or possible functional relationships for SMA in the bovine testis have been reported so far.In the testis, Sertoli cells support germ cells and also constitute the seminiferous tubules. Sertoli cells posses a highly organized cytoskeleton, and vimentin, the most common intermediate filament [7,8], is located around the nucleus and provides it with structural support [5,[9][10][11]. During pubertal processes, mammalian Sertoli cells undergo transformation [12,13] that is likely related to cytoskeletal changes [11,14]. Increases in Sertoli cell vimentin in the prepubertal ovine testis [5] and changes of its distribution area during postnatal development in rats [11] have indicated vimentin activity during postnatal development. Although perinuclear localization of vimentin has been demonstrated in the pre-Sertoli and adult Sertoli cells of the bovine testis [10,15], its dist...

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Section: Discussionsupporting

confidence: 93%

Postnatal Developmental Changes in Immunohistochemical Localization of .ALPHA.-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testes

Devkota

Sasaki

Takahashi³

et al. 2006

Journal of Reproduction and Development

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“…Their roles in cellular structure and function include maintaining cell shape and polarity, positioning of intracellular organelles, forming of cytoplasmic extensions and anchoring of organelles to the plasma membrane. The localization of testicular cytoskeletal proteins in several species has been immunohistochemically examined, including pig [33], sheep [32], buffalo [15], cow [18], lesser mouse deer [30], camel [29], Japanese black bear [23] and rat [40] to elucidate the functional roles of the cytoskeletal proteins. These studies revealed that the testicular localization of each cytoskeletal protein was different among species and the distribution pattern changed with testicular development.…”

mentioning

confidence: 99%

Immunolocalization of Cytoskeletal Proteins in the Testes of Two Asian Cervids: Water Deer (Hydropotes inermis) and Reeves’ Muntjac (Muntiacus reevesi)

Sohn

Sasaki

Yasuda

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. In order to gather basic reproductive information of the water deer and Reeves' muntjac, the immunolocalization of the cytoskeleton proteins in the testes and epididymides of these two species was investigated. The distribution pattern of cytoskeletal proteins in these two species was similar. The desmin was detected in the peritubular myoid cells of the testes and the sub-epithelial cells of the epididymal ducts. Vimentin was observed in the myoid cells, Leydig cells and perinuclear region of the Sertoli cells. Intense immunoreactions for α-smooth muscle actin were restricted to the smooth vascular muscle cells and the peritubular myoid cells in the testes. From the present results, it appears that these distribution patterns of cytoskeletal proteins may be common in the cervids. The water deer (Hydropotes inermis) and Reeves' muntjac (Muntiacus reevesi) both belong to the family Cervidae (order Artiodactyla, suborder Ruminantia). The water deer shows anatomical differences from other deer species by phylogenic evidences, including the absence of antlers and the existence of well-developed upper canines in the male [10,24]. There are two subspecies: the Chinese water deer (H. i. inermis) and the Korean water deer (H. i. argyropus). Although the status of the Chinese water deer in China has declined drastically [39], in contrast, the over-populated situation in South Korea has caused various conflicts to human life, including severe agricultural damage.Reeves' muntjac is one of the muntjac species and can be found in southeastern China and Taiwan as their habitat. The males have antlers, and their upper canines are apparent and protrude from the side of their mouths. This species was introduced into Japan as an exhibition animal and naturalized in the southern part of Chiba prefecture between the 1960s and 1980s [6] and onto Izu-oshima island in 1970 [27]. In the genus Muntjac, some studies on their reproduction and growth have been performed [11][12][13][14]. Macroscopic and histological observations of the male reproductive organs of the Korean water deer have been performed and demonstrate that the Korean water deer possesses the same four typical accessory reproductive glands as other ruminant species. Seasonal changes in size and weight of the testis were also clearly noted, and the breeding season of the animal has been shown to be from October to December, when the male reproductive functions are most active [31]. The male breeding season in the Japanese population of Reeves' muntjac has not been clearly reported, however, the parturition has been observed throughout the year in Chiba prefecture, Japan [27].The cytoskeleton proteins are comprised of microfilaments (actins), intermediate filaments (desmin and vimentin), microtubules (tubulin), neuro-filament proteins, glial fibrillary acidic proteins and cytokeratin. Their roles in cellular structure and function include maintaining cell shape and polarity, positioning of intracellular organelles, forming of cytoplasmic extensions and anchoring o...

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“…Studies on contractile cells surrounding rete channels and efferent ducts of mammals are rather rare (Baumgarten et al 1971;Viotto et al 1993;Rodríguez et al 1999;Devkota et al 2006), although they can also be expected to play a considerable role in the sperm transport. Contractile cells of the lamina propria of the ductus epididymidis are generally characterised as smooth muscle cells (Baumgarten et al 1971;Whittington et al 2001; see also textbooks on human and veterinary histology).…”

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confidence: 99%

Peritubular Contractile Cells in Testis and Epididymis of the Dog, Canis lupus familiaris

Egger¹,

Witter²

2009

Acta Vet. Brno

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Contractile cells surrounding the tubular system of the mammalian testis and epididymis are supposed to contribute to the initial transport of spermatozoa from the testis to epididymis. Testicular peritubular smooth muscle cells have been characterised in detail especially in rodents and humans. The aim of our study was to assess the distribution of peritubular contractile cells of the canine tubuli seminiferi, rete testis channels, ductuli efferentes, and ductus epididymidis by immunohistochemistry and transmission electron microscopy and to classify these cells with respect to their possible physiological function. The entire tubular system of the canine testis and epididymis is surrounded by contractile cells expressing smooth muscle actin, smooth muscle myosin and desmin, which are enveloped, at least partly, by a basal lamina. Some contractile cells of the tubuli seminiferi, rete channels, and efferent ducts and sometimes also single peritubular cells of the ductus epididymidis express vimentin. Contractile cells of seminiferous tubules and efferent ducts represent an intermediate cell type exhibiting characteristics of both smooth muscle cells (SMC) and myofibroblasts, those of rete channels stellate myofibroblasts, and those of the ductus epididymidis SMC. Differences in structure and arrangement of the contractile cells between seminiferous tubules, rete channels, efferent ducts, and ductus epididymidis suggest different functions. Myofibroblasts and contractile cells similar to them could be mainly responsible for the maintenance of an appropriate tissue turgor, whereas contraction of SMC of the ductus epididymidis might cause propulsion of spermatozoa by peristaltic waves. Myofibroblasts, smooth muscle cells, sperm transport, immunohistochemistry, electron microscopy

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Distribution of keratins, vimentin, and actin in the testis of two South American camelids: Vicuna(Vicugna vicugna)and llama(Lama glama). An immunohistochemical study

Cited by 17 publications

References 30 publications

Postnatal Developmental Changes in Immunohistochemical Localization of .ALPHA.-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testes

Postnatal Developmental Changes in Immunohistochemical Localization of .ALPHA.-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testes

Immunolocalization of Cytoskeletal Proteins in the Testes of Two Asian Cervids: Water Deer (<i>Hydropotes inermis</i>) and Reeves’ Muntjac (<i>Muntiacus reevesi</i>)

Peritubular Contractile Cells in Testis and Epididymis of the Dog, Canis lupus familiaris

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