Distribution of IS5 in bacteria

Schoner, Brigitte E.; Schoner, Ronald G.

doi:10.1016/0378-1119(81)90093-7

Cited by 26 publications

(18 citation statements)

References 13 publications

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“…The clearly defined patterns obtained not only allows their distribution and copy numbers in the various strains to be determined but could also be used to identify and characterize strains (10,16,19,34). With Rhizobium sp., Wheatcroft and Watson (44) used ISRml to characterize Rhizobium strains by restricting total DNA with different enzymes, Southern blotting, and hybridization to ISRml.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors

et al. 1991

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On the basis of an RSF1010-derived broad-host-range vector, three different systems which enable positive detection and isolation of insertion sequence (IS) elements from gram-negative bacteria were constructed. Vectors pSUP104-pheS, pSUP104-rpsL, and pSUP104-sac were used successfully in a number of Rhizobium strains and in Xanthomonas campestris. More than 20 different IS elements were isolated and characterized. The 16 IS elements from Rhizobium meliloti were further used to characterize various R. meliloti strains by hybridization. The resulting hybridization patterns were different for every strain and gave a dear and definite IS fingerprint of each strain. These IS fingerprints can be used to identify and characterize R. meliot strains rapidly and unequivocally, as they proved to be relatively stable. Some of the IS elements were found to be identical when the IS fingerprints from a given strain were compared. This method of IS fingerprinting can also establish whether IS elements are the same, related, or different.Insertion sequence (IS) elements are defined as relatively small mobile genetic entities which, unlike drug resistance transposons, do not contain selectable genes (5). This definition makes it clear that direct selection for the presence of an IS element is usually not possible. IS elements in procaryotes were first identified as causative agents of spontaneous mutations in Escherichia coli (21, 36). Since then, various classes of transposable elements have been found to be natural constituents of many bacterial chromosomes, plasmids, and bacteriophages. As far as gram-negative bacteria are concerned, most IS elements have been isolated from enteric bacteria (mainly E. coli; for reviews and lists of isolated elements, see references 14 and 20). However, there have been a substantial number of reports on IS elements identified in other gram-negative bacterial species, such as Agrobacterium tumefaciens (3,15,24,42), Xanthomonas campestris (22, 23), and various Rhizobium (9,28,32) and Pseudomonas (6,13,35,41,45) strains.In general, these elements have been found more or less by chance because of the capacity to inactivate or activate particular genes. However, there have been successful attempts to screen for transposable elements by means of direct selection procedures (8,15,30,35).In this report, we present the construction and use of three broad-host-range vectors designed to enable systematic searches for the presence of transposable elements in gramnegative bacteria. Advantage was taken of two direct selection systems initially constructed to simplify cloning experiments with E. coli (7, 17), as well as a system described by Gay and coworkers (15). The common principle of these systems is based upon insertional inactivation of a vectorborne dominant sensitivity marker. With pSUP104-pheS and pSUP104-rpsL, this leads to drug resistance. Inactivation of pSUP104-sac allows its host to grow on medium containing 5% sucrose. Both of these events are positively selectable phenotypes in the hosts. (A...

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors

et al. 1991

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“…Microbiol., in press). The IS elements of gram-negative bacteria are frequently distributed throughout a number of genera; IS] (12, 13), IS2, (12), IS5 (12,15), and IS15 (5) are examples of widely disseminated IS elements. IS200 is an example of an IS element that appears to be restricted to the genus Salmonella (6).…”

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“…The lanes contained DNA isolated from L. casei. C257 (1,6,11), MSK248 [C257 lacking pLY101, [2,7,12]), ATCC 393 (3,8,13), ATCC 393 lacking pLZ15 (4,9,14), and C9 (5,10,15). Samples (1 ,ug) of DNA were applied to lanes 1 through 5; lanes 6 through 15 were loaded with various quantities of DNA in order to produce signals of approximately equal intensity.…”

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“…This may be attributable to a common ancestry that existed before the ancient divergence of gram-positive and gram-negative organisms. On the other hand, lateral transfer between strains after phylogenetic divergence has been postulated for IS] (3, 13), IS2 (3), and IS5 (15). Hypothetically, both divergence and distribution may participate in defining the range of an IS element.…”

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Evidence that Lactobacillus casei insertion element ISL1 has a narrow host range

1988

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The 1.3-kilobase-pair insertion element ISLJ, originally isolated from Lactobacillus casei S-1, was found to have an extremely restricted host range. By DNA-DNA hybridizations performed with Southern transfers by using a cloned internal fragment of ISL1 as a molecular probe, it was found that only 3 of 19 L. casei strains examined contained sequences that hybridized to the ISLJ probe. In two of these, the hybridizing sequences were found on lactose plasmids. No homologous sequences were detected in a survey of 14 other Lactobacillus strains (9 species) and 15 strains of other bacteria (8 genera, 12 species).Although insertion sequence (IS) elements are varied, common, and widely distributed among gram-negative bacteria (4), only four IS-like elements in gram-positive bacteria have been identified and sequenced (1,8,14,17 Salmonella (6). The host range, distribution, and frequency of gram-positive IS elements are at present unknown. We report here the results of a survey of 48 bacterial strains, representative of several genera in addition to Lactobacillus, for sequences hybridizing to ISLI (Table 1).DNA was isolated from L. casei S-1 and C-9 by the methods described by . DNA isolation from other Lactobacillus strains, Streptococcus spp., Staphylococcus aureus, and Actinomyces viscosus was by published procedures (2, 7). The procedure used to prepare DNA from all other strains was essentially that described by Marmur (10

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“…After 3 days at 31°C, Bgl+ variants appeared as papillae on the colonies, and the papillae in each of approximately 100 well-separated colonies were counted by using a dissecting microscope. Bgl+ variants arise most frequently because of transposition of an endogenous IS] or IS5 (5 to 10 copies of each are normally present on the E. coli K-12 chromosome [22,28] to the cryptic bgl operon (23 (Table 1). Similar results were obtained for the transposition of TnS from sites in thr, arg, and lys genes to a k prophage in otherwise isogenic rho' and rho-15(Ts) strains (Table 2).…”

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Reduced transposition in rho mutants of Escherichia coli K-12

Datta¹,

Rosner²

1987

J Bacteriol

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Substantially reduced frequencies of transposition for the transposons Tn5 and Tn9 and the insertion sequences IS] and IS5 were observed in several rho mutants of Escherichia coli K-12 compared with those observed in their isogenic wild-type counterparts. The lower transposition frequencies could be due to decreased supercoiling of DNA, to altered expression of required genes, or The decreased frequency of bgl activation could be due to an overall reduction of transposition frequencies in rho mutants or to an inability of IS] and IS5 to transpose to the

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Distribution of IS5 in bacteria

Cited by 26 publications

References 13 publications

Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors

Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors

Evidence that Lactobacillus casei insertion element ISL1 has a narrow host range

Reduced transposition in rho mutants of Escherichia coli K-12

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