Distribution of Intrarenal Blood Flow after Renal Denervation in the Dog

Nomura, Gakushi; Y, Kibe; Arai, Shirõ; Uno, Denji; Takeuchi, Jugoro

doi:10.1159/000180593

Cited by 7 publications

(5 citation statements)

References 15 publications

(32 reference statements)

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“…Middle and outer cortical flows, however, were not statistically different, indicating that any redistribution of flow away from the deep cortex secondary to denervation was minimal. This corroborates the conclusions of others on anaesthetised (Nomura et al 1976;Bonjour et al 1969) as well as unanaesthetized (Pomeranz et al 1968; Klisiecki, Pickford, Rothschild & Verney, 1931) preparations, that adrenergic influences on cortical flow during resting conditions are small, if any.…”

Section: Discussionsupporting

confidence: 91%

“…The controversy is unresolved by denervation studies. Some authors (Nomura, Kibe, Arai, Uno & Takeuchi, 1976;Bonjour, Churchill and Malvin, (1969) failed to demonstrate a change in renal blood flow after denervation although others have reported redistribution towards the deep cortex (Benscath, Szalay & Takacks, 1971) as also the outer cortex (Lackner & McKay, 1971).…”

Section: Introductionmentioning

confidence: 99%

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The effects of renal denervation and meclofenamate on renal blood flow regulation in conscious rabbits

Beilin¹,

Bhattacharya²

1979

The Journal of Physiology

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SUMMARY1. The effect on renal blood flow regulation of unilateral renal denervation followed by administration of a prostaglandin synthesis inhibitor has been studied in nine conscious rabbits.2. Renal blood flow distribution was estimated using radioactive microspheres injected via a pre-implanted left ventricular cannula. Cardiac output was estimated by thermodilution. Measurements were made before and after intravenous injection of Meclofenamate, Parke Davis Ltd (4 mg/kg).3. Renal denervation caused a fall in renal cortical catecholamine concentration of approximately 50 % by 48 hr. 4. Resting renal blood flow did not differ significantly between denervated and innervated kidneys. 5. Meclofenamate caused a significant rise in arterial pressure from a mean of 59 to 70 mmHg. In innervated kidneys, Meclofenamate caused a fall in renal blood flow from 8-1 + 0-6 (mean + S.E. of mean) to 6-5 + 0-4 ml./min.g kidney weight while in denervated kidneys it caused a fall from 8-9 + 0-7 to 6-25 + 0 3 ml./min.g.After Meclofenamate flow was redistributed away from the inner cortex in the innervated kidney and paradoxically towards the inner cortex in the denervated one.6. It was concluded that in conscious animals, resting sympathetic tone has little effect on renal blood flow. The exaggerated reduction in flow in the outer cortex of the denervated kidney following Meclofenamate may indicate denervation hypersensitivity to circulating catecholamines unmasked by reduced synthesis of vasodilator prostaglandins. Alternatively, renal sympathetic nerves may normally prime prostaglandin synthesis in the outer cortex.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

The effects of renal denervation and meclofenamate on renal blood flow regulation in conscious rabbits

Beilin¹,

Bhattacharya²

1979

The Journal of Physiology

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“…However, the systemic blood pressure was not affected by the renal denervation. Previous studies also showed that the denervation diuresis occurred without significant changes in glomerular filtration rate, renal blood flow and distribution of intrarenal blood flow [12, 13]. It is unlikely that the decreased expression of AQP channels in the denervated kidney should be attributed to altered local hemodynamics.…”

Section: Discussionmentioning

confidence: 90%

Decreased Expression of Aquaporin Water Channels in Denervated Rat Kidney

Lee¹,

Yoo²,

Kim

et al. 2006

Nephron Physiol

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Aims: A neural mechanism regulating aquaporin (AQP) water channels in the kidney was investigated. Methods: Male Sprague-Dawley rats were used. Renal denervation was induced by painting the renal vessels with 10% phenol. The expression of AQP1–4 proteins was determined in the denervated and contralateral kidneys. The expression was also examined in rats which were renally denervated and subjected to water restriction or deoxycorticosterone acetate (DOCA)-salt treatment. Results: Following the unilateral denervation, tissue contents of norepinephrine were significantly decreased in the denervated kidney, while increased in the contralateral kidney. Accordingly, the expression of AQP1–4 proteins was decreased by 15–40% in the denervated kidney, and increased by 30–50% in the contralateral kidney. Immunohistochemistry of AQP2 confirmed its decreases in the denervated kidney and increases in the contralateral kidney. In bilaterally denervated rats, the urine flow increased along with decreased osmolarity. The water restriction increased the expression of AQP channels, however, the magnitude of which was lower in the denervated than in the contralateral kidney. Renal denervation decreased the degree of DOCA-salt hypertension, along with lower expression of AQP channels. Conclusion: It is suggested that the sympathetic nerve should play a specific excitatory role in the regulation of AQP channels in the kidney.

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“…2 and 3). Interruption of the spontaneous activity by denervation of the kidney has been found in most investigations to result in a diuresis and natriuresis, without any detectable alterations in renal blood flow or glomerular filtration rate (Takics et al 1971, Bencsath et al 1972, Bello-Reuss et al 1975Nomura et al 1976, DiBona & Rios 1980. Comparable results have been obtained on stimulation of the renal nerves at a low level, i.e.…”

Section: Biological Considerationsmentioning

confidence: 98%

“…As the normal spontaneous activity of the renal nerves is 1-2 Hz (Kendrick et al (1972) and Thoren outer zone & Riksten (1979)), the nerves would control the urinary output via the regulation of tubular fluid reabsorption. Consequently, denervation of the kidney should induce a diuresis and natriuresis owing to the effects on the tubular epithelial cells and not due to any haemodynamic changes (Takhcs et al 1971, Bencshth et al 1972, Bello-Reuss et al 1975, Nomura et al 1976, DiBona & Rios 1980. In a previous study from this laboratory (Hermansson 1981) we suggested that even a low level of stimulation will be accompanied by a vasoconstriction.…”

mentioning

confidence: 92%

The cortical and medullary blood flow at different levels of renal nerve activity

Hermansson

Öjteg

Wolgast

1984

Acta Physiologica Scandinavica

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The effect of (1) renal denervation and (2) stimulation of the renal nerve on the regional renal blood flow were determined by the Rb uptake method. Under control conditions the total renal blood flow was 3.64 +/- 0.09 ml X min-1 X g-1 tissue increasing significantly (p less than 0.02) to 4.39 +/- 0.28 ml X min-1 X g-1 after denervation. Upon stimulation of the peripheral portions of the sectioned renal nerves the blood flow decreased almost linearly with the frequency of stimulation reaching 0.99 +/- 0.24 ml X min-1 X g-1 at 10 Hz. Utilizing the relation between blood flow and stimulation frequency the control blood flow correspond to a spontaneous activity of 1.5 Hz. As expected the cortical blood flow responded in the same way as for the total renal blood flow. In the renal medulla denervation gave a much more pronounced response where e.g. the inner medullary flow increased from 0.88 +/- 0.09 to 1.30 +/- 0.16 ml X min-1 X g-1, i.e. a 50% increase (p less than 0.05). Stimulation with 2 Hz produced a steep fall in the blood flow, whereafter it decreased linearly with the stimulation frequency reaching 0.11 ml X min-1 X g-1 at 10 Hz stimulation. This demonstrates again that the renal medulla is sensitive to renal nerve activity primarily in the low level range. It should be remarked, however, that the 86-Rb uptake method reflects the effective blood flow, which might differ from the blood flow in absolute terms.(ABSTRACT TRUNCATED AT 250 WORDS)

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Distribution of Intrarenal Blood Flow after Renal Denervation in the Dog

Cited by 7 publications

References 15 publications

The effects of renal denervation and meclofenamate on renal blood flow regulation in conscious rabbits

The effects of renal denervation and meclofenamate on renal blood flow regulation in conscious rabbits

Decreased Expression of Aquaporin Water Channels in Denervated Rat Kidney

The cortical and medullary blood flow at different levels of renal nerve activity

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