Distribution of Integrons and Gene Cassettes Among Uropathogenic and Diarrheagenic <i>Escherichia coli</i> Isolates in Iran

Zeighami, Habib; Haghi, Fakhri; Masumian, Neda; Hemmati, Fatemeh; Samei, Ali; Naderi, Ghazal

doi:10.1089/mdr.2014.0147

Cited by 19 publications

(8 citation statements)

References 23 publications

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“…That the detection rate of dfrA in the present study was low may reflect that P. aeruginosa has a strong ability to respond quickly to the selection pressure of antibiotics. [31][32][33] Although the frequency of isolates resistant to b-lactamases was high, the blaOXA-1 gene cassette was found in only one commensal isolate, suggesting that the genes encoding blactamases are mostly located outside of integrons, which was also observed by Mokracka et al 34,35 The extended-spectrum blactamase resistance genes-blaOXA-1 and blaOXA-101were also found in the present study. These genes can confer resistance to cefepime and IPM, the most commonly used antibiotics nowadays.…”

Section: Discussionsupporting

confidence: 82%

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons in Pseudomonas aeruginosa Strains

Liu

Jia

et al. 2020

Microbial Drug Resistance

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We investigated the antibiotic-resistance phenotypes and molecularly characterized class 1 integron gene cassettes from 113 Pseudomonas aeruginosa isolates from patients. Primers specific for the class 1 integron integrase (intI1) gene were used to screen for these integrons using polymerase chain reactions (PCRs). The variable regions of the integrons were PCR-amplified and sequenced. Sputum was the most common specimen (69.9%; 79/113) followed by aseptic sites (21.2%; 24/113). Of the 113 isolates with phenotypic resistance to the tested antimicrobials, the highest resistances were to ciprofloxacin (CIP) (26.55%), imipenem (IPM) (23.89%), and meropenem (MEM) (23%). Carbapenem-sensitive P. aeruginosa (CS-PA) isolates displayed 23 patterns, and the predominant multidrug resistance phenotype was CIP-levofloxacin (7.23%, 6/83). Carbapenem-resistant P. aeruginosa (CR-PA) isolates displayed 12 patterns, and the predominant multidrug resistance phenotype was IPM-MEM (23.33%, 7/30). Class 1 integrons were detected in 14 (12.4%, 14/113) isolates, 7.22% (6/83) in CS-PA isolates, and 26.67% (8/30) in CR-PA isolates. Six gene cassette arrays were detected, the most prevalent being aacA4-blaOXA101-aadA5 in five isolates (4.4%, 5/113). Seventeen gene cassettes were detected. The most prevalent antibiotic-resistance gene cassettes were aacA4 (6.2%, 7/113), bla OXA-1 , and bla OXA-101. Extended-spectrum b-lactamase resistance genes were detected. Some of the genes carried were similar to those in other species, but some had shared characteristics among the P. aeruginosa isolates. Long-standing drug resistance genes appeared to be under elimination in P. aeruginosa, whereas integrons conferring resistance to commonly used clinical drugs such as b-lactamases, fluoroquinolones, and even carbapenems, as well as some other gene elements, were found to be newly integrated.

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Section: Discussionsupporting

confidence: 82%

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons in Pseudomonas aeruginosa Strains

Liu

Jia

et al. 2020

Microbial Drug Resistance

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“…Regarding the content of the class 1 integrons identified in the Romanian strains, known and largely spread resistance gene cassettes were found and in general their presence correlated well with the phenotypic resistance. Similar to other reports on urinary E. coli strains, among the gene cassettes detected, those carrying genes for combined streptomycin/spectinomycin resistance (aadA genes) and trimethoprim resistance (dfrA genes) were most prevalent (22,(30)(31). Moreover, the inferences drawn from the representative integron variants sequenced indicated dfrA17 -aadA5 cassette array, which was significantly linked to human strains as the most common (32).…”

Section: Discussionsupporting

confidence: 83%

Characterization of antibiotic resistance integrons harbored by Romanian Escherichia coli uropathogenic strains

Oprea¹,

Militaru²,

Ciontea³

et al. 2020

Revista Romana De Medicina De Laborator

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Because little is known about the integrons which constitute an important means of spreading resistance in bacteria circulating in Romania, this study aimed to detect antibiotic resistance gene cassettes embedded in integrons in a convenient collection of 60 ciprofloxacin-resistant Escherichia coli isolates of various phylogroups, associated with community-acquired urinary tract infections. Characterization of the integrons was accomplished by PCR, restriction fragment length polymorphism typing, and DNA sequencing of each identified type. More than half of the tested E. coli strains were positive for integrons of class 1 (31 strains) or 2 (1 strain). These strains derived more frequently from phylogenetic groups A (15 of 21 strains), B1 (10 of 14 strains), and F (3 of 4 strains), respectively. While 20 strains carried class 1 integrons which could be assigned to nine types, eleven strains carried integrons that lacked the 3’-end conserved segment. The attempts made to characterize the gene cassettes located within the variable region of the various integrons identified in this study revealed the presence of genes encoding resistance to trimethoprim, aminoglycosides, beta-lactams or chloramphenicol. The evidence of transferable resistance determinants already established in the autochthonous E. coli strains highlights the need for improved control of resistance-carrying bacteria.

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“…A total of 24.2% of class 1 integrons in Mexican E. coli strains from different clinical sources were associated with MDR (Acosta-Pérez et al, 2015); similar data were obtained in our study. The highest presence of class 1 integrons was associated with phylogenetic groups D and B2 in MDR- and XDR-UPEC strains; however, several studies described variable results regarding the association of integrons and phylogenetic groups (Gündogdu et al, 2011; Zeighami et al, 2015). Sequencing the variable region of class 1 and 2 integrons from MDR- and XDR-UPEC strains revealed the presence of genes encoding the antibiotic modifying enzymes aad A1, aad B, aac C, ant 1, dfr A1, and dfr A17.…”

Section: Discussionmentioning

confidence: 99%

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

et al. 2016

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In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40–70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40–70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.

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Distribution of Integrons and Gene Cassettes Among Uropathogenic and Diarrheagenic Escherichia coli Isolates in Iran

Cited by 19 publications

References 23 publications

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons in Pseudomonas aeruginosa Strains

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons in Pseudomonas aeruginosa Strains

Characterization of antibiotic resistance integrons harbored by Romanian Escherichia coli uropathogenic strains

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

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