Distribution of inorganic salts between the coexisting phases of aqueous polymer two-phase systems

Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt

“…It has been observed that waterstructure making and water-structure breaking ions have different affinities for PEG [15]. As a result, water-structure-making anions such as sulphates and phosphates avoid the PEG-rich phase in twopolymer systems and this may have also occurred in polymer-salt systems [16][17][18]. In contrast, water-structure-breaking anions such as Cl − seem to favour the PEG-rich phase.…”

Application of integral‐equation theory to aqueous two‐phase partitioning systems

“…Most of these previous modeling efforts have focused on a description of nonelectrostatic forces, in particular excluded-volume forces, affecting phase diagrams and partition coefficients. For example, an 1974a; Reitherman et al, 1973;Bamberger et al, 1984;Brooks et al, 1984;Sharp et al, 1986;Zaslavsky et al, 1978Zaslavsky et al, -1983Zaslavsky et al, , 1986Zaslavsky et al, -1988Zaslavsky et al, , 1991. For realistic engineering applications, it is essential that electrostatic forces be included in any model for aqueous solutions containing proteins.…”

Oxidative renaturation of hen egg-white lysozyme in polyethylene glycol-salt aqueous two-phase systems

“…It is well known that water structure perturbing agents, such as urea, a commonly used protein denaturant, adversely affect phase separation in aqueous two-phase polymer systems (Johansson and Kopperschlager, 1987;Zaslavsky et al, 1988Zaslavsky et al, , 1991. Addition of urea to these systems makes it necessary to increase the amount of phase forming solutes in order to achieve phase separation.…”