Distribution of immobilized enzymes on porous membranes

Dalvie, Sandeep K.; Baltus, Ruth E.

doi:10.1002/bit.260401006

Cited by 31 publications

(10 citation statements)

References 21 publications

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“…35 GOD catalyzes the oxidation of glucose, in the presence of air, to yield gluconolactone and hydrogen peroxide. In the presence of the enzyme peroxidase, the H 2 0 2 produced oxidizes the reduced form of dianisidine to the red oxidized form.…”

Section: Glucose Oxidase (God)mentioning

confidence: 99%

Enzyme and chemical encapsulation in polymeric microcapsules

Parthasarathy

Martin

1996

J. Appl. Polym. Sci.

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SYNOPSISPolypyrrole microcapsules (prepared via the template method) were used for immobilization of both enzymatic and chemical catalytic systems. Enzymes immobilized include glucose oxidase, catalase, trypsin, subtilisin, and alcohol dehydrogenase. The chemical catalytic system investigated consisted of immobilized Pd nanoparticles for the catalysis of hydrogen peroxide decomposition. Microcapsules loaded with glucose oxidase (GOD) were found to have higher enzymatic activity than that of GOD-loaded thin films, a competing encapsulation method. Trypsin was used to explore the possible leakage of small proteins from the capsules; no leakage was observed. Subtilisin was used to show that these microcapsules can be used in nonaqueous solvents. The effect of the capsule wall thickness on the rate of the enzymatic reaction was also explored.

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Section: Glucose Oxidase (God)mentioning

confidence: 99%

Enzyme and chemical encapsulation in polymeric microcapsules

Parthasarathy

Martin

1996

J. Appl. Polym. Sci.

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“…The standard odianisidine/peroxidase assay was used (32). A larger amount of GOD was loaded into the capsules used for curve "a" than in the capsules used for curve "b".…”

Section: Enzyme Immobilization Is a Critical Issue In The Developmentmentioning

confidence: 99%

Synthesis of polymeric microcapsule arrays and their use for enzyme immobilization

Parthasarathy

Martin

1994

Nature

328

198

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“…The inhibiting effect of hydrogen peroxide on GOD and CAT was further reduced due to the convective flow through the membrane during the enzyme reaction, which continuously removed the generated H 2 O 2 . This is why polymer membranes are considered the most suitable carriers for the immobilization of that enzyme couple 40–45…”

Section: Resultsmentioning

confidence: 99%

Polyacrylonitrile Enzyme Ultrafiltration and Polyamide Enzyme Microfiltration Membranes Prepared by Diffusion and Convection

Dayal¹,

Godjevargova²

2005

Macromolecular Bioscience

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Glucose oxidase (GOD) and catalase (CAT) were covalently immobilized onto three types of polyacrylonitrile (PAN 1, PAN 2, and PAN 3) ultrafiltration (UF) membranes with different pore sizes and one type of polyamide (PA) microfiltration (MF) membrane by the bifunctional reagent, glutaraldehyde. The initial membranes were pre-modified to generate active amide groups in the PAN membranes and active amino groups in the PA membranes. The PAN 3 membrane contained the highest amount of active groups, and the membrane PA the lowest. The modified membranes were enzyme-loaded by diffusion and convection (UF). The effect of membrane pore size and immobilization methods on enzymatic activity and bound protein were studied. The most effective immobilized system was prepared by diffusion using a PAN 3 membrane as a carrier (bound protein: 0.055 mg/cm(2), relative activity: 87.6%). This membrane had the highest pore size of all the PAN membranes. Despite the highest pore size of PA membrane, the enzyme PA membranes prepared by diffusion showed the lowest amount of bound protein (0.03 mg/cm(2)) and the lowest relative activity (35.38%). This correlates with the lowest amount of active groups found in these membranes. The relative activity was higher for all the enzyme systems loaded by diffusion. The systems prepared by convection of the enzyme solution contained higher amounts of enzymes (0.035-0.13 mg/cm(2) protein), which led to internal substrate diffusion resistance and a decrease in the GOD relative activity (21.55-68.5%) in these systems. The kinetic parameters (V(max) and K(m)) and the glucose conversion of the immobilized systems prepared by diffusion were also studied. [diagram in text].

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Distribution of immobilized enzymes on porous membranes

Cited by 31 publications

References 21 publications

Enzyme and chemical encapsulation in polymeric microcapsules

Enzyme and chemical encapsulation in polymeric microcapsules

Synthesis of polymeric microcapsule arrays and their use for enzyme immobilization

Polyacrylonitrile Enzyme Ultrafiltration and Polyamide Enzyme Microfiltration Membranes Prepared by Diffusion and Convection

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