Distribution of human papillomavirus genotypes in women with high-grade cervical intraepithelial lesions and cervical carcinoma and analysis of human papillomavirus-16 genomic variants

Abstract

Aim To analyze the distribution of high-risk human papillomavirus (HR-HPV) genotypes and the diversity of HPV-16 genomic variants in Croatian women with high-grade squamous intraepithelial lesions (HSIL) and cervical carcinoma. Methods Tissue biopsy specimens were obtained from 324 women with histopathologically confirmed HSIL or cervical carcinoma, 5 women with low-grade SIL, and 49 women with negative histopathology. HR-HPV DNA was detected with Ampliquality HPV-type nucleic-acid hybridization assay, which identifies 29 different HPV genotypes. HPV-16 genomic variants were analyzed by an in-house sequencing. Results The most common HPV type in women with HSIL was HPV-16, detected in 127/219 (57.9%) specimens. HPV-16 was also the dominant type in squamous cell cervical carcinoma (46/69 or 66.7%) and in adenocarcinoma (18/36 or 50.0%). Out of 378 patients, 360 had HR-HPV (282 single infections and 79 multiple infections), 3 (0.8%) patients had low-risk HPV, and 15 (4%) tested negative. HPV-16 variants were determined in 130 HPV-16 positive specimens, including 74 HSIL and 46 carcinoma specimens. In HSIL specimens, 41 distinct variants were found, 98.6% belonging to the European branch and 1.4% belonging to the African branch. In cervical carcinoma specimens, 95% isolates grouped in 41 variants belonging to the European branch, one isolate (2.5%) belonged to the North American, and one (2.5%) to the Asian-American branch. Conclusion HPV-16, mainly belonging to the European branch, was the most frequent HPV genotype in women from Croatia with histologically confirmed HSIL and cervical cancer.

Distribution of vaccine-related high-risk human papillomaviruses and their impact on the development of cervical dysplasia in women in Montenegro

“…We found an increased incidence of multiple HPV infections in women with cervical dysplasia compared to those without dysplasia (31.1 and 22.6%, respectively); however, there was no association between multiple infections and dysplasia. This is supported by results from other studies [22,30,35]. In both the study and control groups, single infection was more common than multiple infections, but a different prevalence may be observed by expanding the spectrum of HR-HPV genotypes and testing for genotypes that are uncommon or have low oncogenic potential.…”

“…Other studies have shown that HPV 31 was one of the most common HR genotypes. As in Montenegro, HPV 31 was the third most common genotype in Serbia [18] and Bulgaria (10.7 and 12.2%, respectively) [16]; however, in Croatia [30], Bosnia and Herzegovina [22], and Kosovo [19] it was the second most common genotype (12.6, 14.3, and 15.4%, respectively).…”

Human Papillomavirus 16 Lineage D is Associated with High Risk of Cervical Cancer in the Brazilian Northeast Region

“…8 A recent study on Croatian women confirmed that HPV16, mainly belonging to the European branch, was most frequently associated with histologically confirmed high-grade intraepithelial lesions (CIN2 or CIN3) and cervical cancer. 28 In the present study, the lineage A of HPV16 was the most frequently detected lineage, and although it was associated with high-grade lesions (CIN2/CIN3), we could not demonstrate a difference between these high-grade lesions and cervical cancer. Another study conducted in a different region in Brazil also found a high prevalence of the lineage A of HPV16 in samples of high-and low-grade lesions, including cervical cancer.…”

“…A recent study on Croatian women confirmed that HPV16, mainly belonging to the European branch, was most frequently associated with histologically confirmed high-grade intraepithelial lesions (CIN2 or CIN3) and cervical cancer. 28…”

Human Papillomavirus 16 lineage D is associated with high risk of cervical cancer in the Brazilian Northeast region

“…A recent study with women in Croatia con rms the predominance of HPV-16, mainly belonging to the European branch, as the most frequently associated with histologically con rmed HSIL and cervical cancer [29].…”