Distribution of human colonic dendritic cells and macrophages

Abstract

SUMMARYTo define the phenotype of intestinal dendritic cells and macrophages, resected colonic specimens were used to obtain lamina propria cell suspensions by EDTA treatment, then enzymatic digestion. The phenotype of dendritic cell-enriched suspensions was compared with that of macrophage-enriched populations by immunocytochemistry using the avidin-biotin-peroxidase (ABC) system and immunoelectron microscopy. Dendritic cells expressed HLA-DR (L243) and HLA-DQ-associated (RFD1) antigens and CD68 in a perinuclear distribution. Staining for S100 was weak or absent. Macrophages also expressed HLA markers (L243 and RFD1) and CD68. The 25F9 antigen was expressed strongly, whilst CD14 was absent from cells isolated from non-inflamed tissues. To determine their anatomic distribution, immunohistochemistry was performed using single-and double-labelling techniques (ABC

Network of dendritic cells within the muscular layer of the mouse intestine

“…Different DC subsets have been described in the intestine, including the lamina propria and Peyer's patches (16,26,27). In general, the expression of MHC-II and the characteristic irregular morphology have been used to identify these cells in situ (16,28).…”

IL‐10 Producing CD14low Monocytes Inhibit Lymphocyte‐Dependent Activation of Intestinal Epithelial Cells by Commensal Bacteria

“…Intestinal monocytes, which constitute 10 20% of mononuclear cells in the lamina propria, differ markedly in phenotype and function from peripheral blood monocytes (16,40). The classical monocyte-specific surface antigens CD14 (LPS co-receptor), CD11b (complement receptor 3, CR3) and CD16 (FcγIII receptor) are expressed at low levels in the normal intestinal mucosa.…”

Migration and Maturation of Human Colonic Dendritic Cells

“…However, little is known about DC in the human intestine. Cell populations with phenotypic properties consistent with DC have been identified in a number of immunocytochemical studies (12)(13)(14), and partially enriched populations of these cells have been shown to stimulate primary T cell responses in a primary allogeneic MLR (15). Detailed analysis of the phenotype and function of these cells is currently lacking.…”