Distribution of HPV 16 E6 gene variants in screening women and its associations with cervical lesions progression

Tan, Guichun; Duan, Meng; Yan-e, LI; Zhang, Na; Zhang, Wenwen; Li, Bohan; Qu, Pengpeng

doi:10.1016/j.virusres.2019.197740

Cited by 12 publications

(19 citation statements)

References 44 publications

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“…The actually considered 17 HR-HPVs are presented as bold red dots. (80,(96)(97)(98)(99)(100), as a similar description was established for the inter-population variability found in HPV 18, 33, and 45 (76,77,101,102). This inter-population variability made it possible to explain that HPVs from the same type induce different gene expression profiles associated with a certain disease occurrence risk, and thus to understand why in some regions the burden of HPV-associated diseases is higher than in others, for the same variants, as presented previously (100).…”

Section: Hpv Genetic Variability and Prevalence Distribution Of Hpv Infection And Related Diseasessupporting

confidence: 66%

“…It appears that this L1 polymorphism occurs within the molecular bases involved in the assembly of the capsid in the form of VLPs (polymorphism in h4, β, and J regions) (164), raising the hypothesis that the 3D structure VLPs could also vary depending on the geographic variant, and therefore introduce significant variability in the oncogenicity associated with the capsid structure. In addition to studies based on L1 genes and their roles, this effect has also been demonstrated in the LCR genes and some early proteins (E) involved in viral functions (76,77,80,(96)(97)(98)(99)(100)(101)(102). Taken together, this polymorphic variability and the associated oncogenic potential positively correlate with the distribution data of HPV-associated disease burden and thus explains why the HPV-related burden is higher in developing regions.…”

Section: Discussionmentioning

confidence: 87%

“…Moreover, studies have shown that in addition to the genetic variability present within the primary sequence of the L1 proteins, allowing an HPV type-based classification (α-HPV 16, 18, 45, etc.) (Figure 2) (94,95), there is a nonnegligible variability within variants of identical HPV types but from distinct regions (80,96,(96)(97)(98)(99)(100), which can be observed in the same geographic region due to human migration (163,164).…”

Section: Discussionmentioning

confidence: 99%

“…(80,(96)(97)(98)(99)(100), as a similar description was established for the inter-population variability found in HPV 18, 33, and 45 (76,77,101,102). This inter-population variability made it possible to explain that HPVs from the same type induce different gene expression profiles associated with a certain disease occurrence risk, and thus to understand why in some regions the burden of HPV-associated diseases is higher than in others, for the same variants, as presented previously (100). For instance, it has been reported that the African variants of HPVs (especially HR-HPVs) are the most virulent (80), explaining why HR-HPVs like 31 and 58 (more specifically HPV 16) are highly associated with infection progression and oncogenicity (103,104), and despite being less prevalent in underdeveloped countries like in Africa (Table 1), are associated with higher morbi-mortality (warts, CIN2/3, cancers, and death).…”

Section: Hpv Genetic Variability and Prevalence Distribution Of Hpv Infection And Related Diseasesmentioning

confidence: 99%

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Epidemiology and Burden of Human Papillomavirus and Related Diseases, Molecular Pathogenesis, and Vaccine Evaluation

Kombe

Zahid

et al. 2021

Front. Public Health

314

276

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Diagnosed in more than 90% of cervical cancers, the fourth deadliest cancer in women, human papillomavirus (HPV) is currently the most common pathogen responsible for female cancers. Moreover, HPV infection is associated with many other diseases, including cutaneous and anogenital warts, and genital and upper aerodigestive tract cancers. The incidence and prevalence of these pathologies vary considerably depending on factors including HPV genotype, regional conditions, the study population, and the anatomical site sampled. Recently, features of the cervicovaginal microbiota are found to be associated with the incidence of HPV-related diseases, presenting a novel approach to identify high-risk women through both blood and cervical samples. Overall, the HPV repartition data show that HPV infection and related diseases are more prevalent in developing countries. Moreover, the available (2-, 4-, and 9-valent) vaccines based on virus-like particles, despite their proven effectiveness and safety, present some limitations in terms of system development cost, transport cold chain, and oncogenic HPV variants. In addition, vaccination programs face some challenges, leading to a considerable burden of HPV infection and related diseases. Therefore, even though the new (9-valent) vaccine seems promising, next-generation vaccines as well as awareness programs associated with HPV vaccination and budget reinforcements for immunization are needed.

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Section: Hpv Genetic Variability and Prevalence Distribution Of Hpv Infection And Related Diseasessupporting

confidence: 66%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Hpv Genetic Variability and Prevalence Distribution Of Hpv Infection And Related Diseasesmentioning

confidence: 99%

See 2 more Smart Citations

Epidemiology and Burden of Human Papillomavirus and Related Diseases, Molecular Pathogenesis, and Vaccine Evaluation

Kombe

Zahid

et al. 2021

Front. Public Health

314

276

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“…Tan et al (2019) reported that European prototype E-T350 was the most prevalent (82.76%) followed by Asian (As) variant. In patients with suspected cervical lesions the most prevalent variant was As variant (54.9%) by increasing significance with severity of cervical diseases [26].…”

Section: Discussionmentioning

confidence: 98%

Role of HPV 16 variants among cervical carcinoma samples from Northeastern Brazil

et al. 2020

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Background: Cervical cancer is the fourth most common type of cancer affecting women globally. In Brazil, it is the third most frequent type of cancer in women and HPV is present in approximately 90% of cases. Evidence suggests that variants of HPV 16 can interfere biologically and etiologically during the development of cervical cancer. Methods: Cervix tumor fragments were collected, their DNA was extracted, and nested PCR was used to detect HPV. Positive samples were sequenced to determine the viral genotype. To characterize the HPV 16 strains, positive samples PCR was used to amplify the LCR and E6 regions of the HPV 16 virus. Results: Data from 120 patients with cervical cancer were analyzed. Most women were between 41 and 54 years of age, had schooling until primary school, a family income between 1 and 2 times the minimum wage and were married/in a consensual union. There was no statistically significant association between HPV or socio-demographic variables and risk factors for cervical cancer (P < 0.05). HPV was present in 88 women (73%). The most prevalent types were HPV 16 (53.4%), HPV 18 (13.8%), HPV 35 (6.9%) and HPV 45 (5.7%). Of the 47 HPV 16 positive cases, variant A (49%) was present in 23 samples, followed by variant D in 20 cases (43%), and variants B and C in 2 cases each (4%). The most prevalent histological type of HPV 16 tumors was squamous cell carcinoma, followed by adenocarcinoma. There was a statistically significant association between HPV 16 variants and the tumors' histological types (P < 0.001).

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Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina

Elina

Juan

Javier

et al. 2021

Journal of Medical Virology

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The province of Misiones is considered a region with a high mortality rate due to cervical cancer (CC). To gain insight into this problem, we explored the association between genetic variation in the E6 and E7 oncogenes of HPV16 and the risk of CC.We studied 160 women with cytological diagnoses of negative for intraepithelial lesion or malignity, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion/CC and a positive test for HPV16 infection. The genetic characterization of E6 and E7 genes was undertaken through PCR amplification and direct Sanger sequencing. Phylogenetic classification was conducted using Bayesian methods. To estimate the odds ratio (OR) for an association between genetic variants in the E6 and E7 genes and the risk of CC, we used ordinal logistic regression adjusted by age. The final data set comprised 112 samples. Diagnostic single-nucleotide polymorphisms (SNPs) and phylogenetic trees confirmed the presence of Lineage A (95.5%) and D (4.5%) in the samples. For the E6 gene, we identified eleven different sequences, with the most common ones being Lineage A E6 350G (58.9%) and E6 350T (37.5%). The E6 350G was associated with progression to HSIL/CC, with an OR of 19.41 (4.95-76.10). The E7 gene was more conserved than E6, probably due to the functional constraints of this small protein.Our results confirmed the association of the E6 350G SNP with a higher risk of developing CC. These data will contribute to understanding the biological bases of CC incidence in this region.

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Distribution of HPV 16 E6 gene variants in screening women and its associations with cervical lesions progression

Cited by 12 publications

References 44 publications

Epidemiology and Burden of Human Papillomavirus and Related Diseases, Molecular Pathogenesis, and Vaccine Evaluation

Epidemiology and Burden of Human Papillomavirus and Related Diseases, Molecular Pathogenesis, and Vaccine Evaluation

Role of HPV 16 variants among cervical carcinoma samples from Northeastern Brazil

Genetic variation in the E6 and E7 genes of human papillomavirus type 16 in northeastern Argentina

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