Distribution of hepatitis c virus (hcv) genotypes in patients with chronic infection from Rondônia, Brazil

Abstract

BackgroundHepatitis C virus (HCV) is an important human pathogen affecting around 3% of the human population. In Brazil, it is estimated that there are approximately 2 to 3 million HCV chronic carriers. There are few reports of HCV prevalence in Rondônia State (RO), but it was estimated in 9.7% from 1999 to 2005. The aim of this study was to characterize HCV genotypes in 58 chronic HCV infected patients from Porto Velho, Rondônia (RO), Brazil.MethodsA fragment of 380 bp of NS5B region was amplified by nested PCR for genotyping analysis. Viral sequences were characterized by phylogenetic analysis using reference sequences obtained from the GenBank (n = 173). Sequences were aligned using Muscle software and edited in the SE-AL software. Phylogenetic analyses were conducted using Bayesian Markov chain Monte Carlo simulation (MCMC) to obtain the MCC tree using BEAST v.1.5.3.ResultsFrom 58 anti-HCV positive samples, 22 were positive to the NS5B fragment and successfully sequenced. Genotype 1b was the most prevalent in this population (50%), followed by 1a (27.2%), 2b (13.6%) and 3a (9.0%).ConclusionsThis study is the first report of HCV genotypes from Rondônia State and subtype 1b was found to be the most prevalent. This subtype is mostly found among people who have a previous history of blood transfusion but more detailed studies with a larger number of patients are necessary to understand the HCV dynamics in the population of Rondônia State, Brazil.

Hepatitis C screening, diagnosis, and cascade of care among people aged > 40 years in Brasilia, Brazil

“…The most common genotypes in the present study were genotypes 1 (1a and 1b), 3, and 2, similar to those reported in several previous studies in Brazil, including a multicenter evaluation [13,[38][39][40][41].…”

Simultaneous determination of HCV genotype and NS5B resistance associated substitutions using dried serum spots from São Paulo state, Brazil

“…It confirms the worldwide panorama since the widespread HCV genotypes are 1 (44 %) and genotype 3 (25 %) [65]. The prevalence of HCV genotype distribution in this study is also in accordance with previous studies performed in Brazil [66][67][68]. Recently, Mutini and colleagues described a high prevalence of genotypes 1a (41%), 1b (30%), 3a (24%), and 2 (4 %) in about 29071 of either healthy people or blood donors in Brazil.…”

Genetic Diversity of HCV in Brazil

“…Partial NS5B gene sequences were obtained from a total of 254 HCV strains (subtype 1a=99, subtype 1b=76, subtype 3a=61, genotype 2=10, genotype 4=6 and subtype 5a=2) isolated in the south (Rio Grande do Sul), south-east (Rio de Janeiro), central-west (Goiás and Mato Grosso do Sul) and north (Amapá) Brazilian regions, between 2002 and 2011. These new sequences were added along with 444 Brazilian HCV NS5B gene sequences (subtype 1a=222, subtype 1b=94, subtype 3a=124, genotype 2=3 and genotype 5=1) isolated in the south-east (São Paulo), central-west (Goiás, Mato Grosso and Mato Grosso do Sul) and north (Rondônia) regions that were previously published [18,22,23,[25][26][27]. The Brazilian HCV sequences were analysed together with global reference sequences available at the Los Alamos HCV database [28] that matched the selected genomic region.…”