Distribution of Helicobacter pylori organisms in the stomachs of children with H. pylori infection

Elitsur, Yoram; Lawrence, Zandra; Triest, William E.

doi:10.1053/hupa.2002.129201

Cited by 25 publications

(23 citation statements)

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“…Elitsur et al have been shown that the gastric antrum demonstrated the highest sensitivity rate for the detection of H. pylori organism in children [44]. Elitsur et al have been shown that the gastric antrum demonstrated the highest sensitivity rate for the detection of H. pylori organism in children [44].…”

Section: Discussionmentioning

confidence: 99%

“…In children, H. pylori infection mainly involves gastric antrum. Elitsur et al have been shown that the gastric antrum demonstrated the highest sensitivity rate for the detection of H. pylori organism in children [44]. Due to the shorter time of infection and antrum predominate involvement, the spectrum of H. pylori infection in children is generally milder than in adults that chronic atrophic gastritis or gastric cancer is rarely seen in pediatric ages.…”

Section: Discussionmentioning

confidence: 99%

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Helicobacter pylori Infection in Children: Nutritional Status and Associations with Serum Leptin, Ghrelin, and IGF‐1 Levels

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori Infection in Children: Nutritional Status and Associations with Serum Leptin, Ghrelin, and IGF‐1 Levels

et al. 2015

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“…pylori density has been found to correlate with the degree of gastric-mucosal inflammation in children (Elitsur et al, 2002). Different authors have used different methods for scoring H. pylori density, including histology, culture, real-time quantitative PCR-based techniques or the urea breath test (Elitsur et al, 2002;Lai et al, 2003;Atherton et al, 1996;Abbas et al, 2001;He et al, 2002). One disadvantage of the invasive tests can be the patchy distribution of H. pylori in gastric mucosa (Vinette et al, 2004); however, H. pylori density has often been evaluated by invasive tests.…”

Section: Resultsmentioning

confidence: 99%

Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

Boyanova

Lazarova²,

Jelev³

et al. 2007

Journal of Medical Microbiology

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The aims of the study were to evaluate the incidence of Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children from 1996 to 2006, to analyse the performance of diagnostic tests, and to look at H. pylori density in specimens by culture. Antral specimens from children with chronic gastritis (n5513), peptic ulcers (n554) and other diseases (n591) were evaluated by direct Gram staining (DGS), in-house rapid urease test (RUT) and culture. The living environment and semi-quantitative H. pylori density were assessed in 188 and 328 children, respectively. H. pylori infection was found in children with ulcers (77.8 %), chronic gastritis (64.5 %) and other diseases (36.3 %). Half (51.4 %) of patients aged 1-5 years and 77.4 % of those aged 16-17 years were H. pylori-positive. Of all children, 328 (49.8 %) showed positive DGS, 184 (28 %) had a positive RUT, and 386 (58.7 %) were culture-positive. Unlike gastric mucus specimens, frozen biopsy specimens provided reliable diagnosis. H. heilmannii was observed in two (0.3 %) children. High H. pylori density (growth into all quadrants of plates) was found in 18 % of 328 children evaluated, involving 31 % of ulcer and 16.7 % of non-ulcer patients. H. pylori infection was more common in rural children with chronic gastritis (91.3 %) than in the remainder (66.7 %). In conclusion, H. pylori infection was common in symptomatic Bulgarian children. The infection prevalence was .77 % in patients aged 16-17 years, in children with a duodenal ulcer, and in rural patients. H. heilmannii infection was uncommon. The performance of the bacterial culture was good. The impact of H. pylori density on the clinical expression and eradication of the infection requires further evaluation. The results highlight the need for routine H. pylori diagnosis in rural children with chronic gastritis. INTRODUCTIONHelicobacter pylori infection is associated with gastroduodenal diseases in humans (Matysiak-Budnik et al., 2006). The incidence of the infection in children ranges from 10 to 80 %, and is high in developing and some other countries (Torres, 2000; Janulaityte-Gunther et al., 2005;Kato et al., 2004). H. pylori infection is most often intrafamilial and spreads by oral-oral or faecal-oral transmission (Megraud, 2003;Mladenova et al., 2006). Helicobacter heilmannii can also infect the human stomach. The incidence (usually ,1 %) of this zoonotic infection in dyspeptic patients is much lower than that caused by H. pylori (Ierardi et al., 2001;Okiyama et al., 2005). There are, however, only a few reports concerning H. heilmannii incidence in children (Coman et al., 1996;Mention et al., 1999; Sykora et al., 2004).The aims of the present study were to assess the incidence and characteristics of H. pylori and H. heilmannii infections in untreated Bulgarian children over a period of 10 years, according to age, sex, disease and living environment; to analyse the performance of diagnostic tests according to the type of antral specimen; and to evaluate H. pylori density in the biopsy spec...

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“…Modified Giemsa staining was used to detect H. pylori in gastric mucous tissues according to a previous report [22].…”

Section: Methodsmentioning

confidence: 99%

Involvement of Aquaporin 3 in Helicobacter pylori-Related Gastric Diseases

et al. 2012

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BackgroundGastric cancer is one of the most common and lethal malignant cancers worldwide, and numerous epidemiological studies have demonstrated that Helicobacter pylori (H. pylori) infection plays a key role in the development of gastric carcinomas. Our previous studies showed that aquaporin 3 (AQP3) is overexpressed in gastric carcinoma and promotes the migration and proliferation of human gastric carcinoma cells, suggesting that AQP3 may be a potentially important determinant of gastric carcinoma. However, the role of AQP3 in H. pylori carcinogenesis is unknown.MethodsThe AQP3 protein and H. pylori were detected in human gastric tissues by immunohistochemistry and modified Giemsa staining respectively. AQP3 knockdown was obtained by small interfering (si) RNA. Western blot assays and RT-PCR were used to evaluate the change of AQP3 in the human gastric cancer AGS and SGC7901 cell lines after co-culture with H. pylori. Sprague Dawley rats were orally inoculated with H. pylori to establish a rat model colonized by H. pylori.ResultsThe present study found that AQP3 expression correlated with H. pylori infection status in gastric cancer tissues and corresponding normal mucosa, and H. pylori co-culture upregulated AQP3 expression in human gastric adenocarcinoma cells in vitro via the extracellular signal-regulated kinase signaling pathway. H. pylori infection also increased AQP3 expression in gastric mucosa colonized by H. pylori in a Sprague Dawley rat model.ConclusionsThese findings provide further information to understand the mechanism of H. pylori carcinogenesis and a potential strategy for the treatment of H. pylori-associated gastric carcinoma.

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Distribution of Helicobacter pylori organisms in the stomachs of children with H. pylori infection

Cited by 25 publications

References 9 publications

Helicobacter pylori Infection in Children: Nutritional Status and Associations with Serum Leptin, Ghrelin, and IGF‐1 Levels

Helicobacter pylori Infection in Children: Nutritional Status and Associations with Serum Leptin, Ghrelin, and IGF‐1 Levels

Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

Involvement of Aquaporin 3 in Helicobacter pylori-Related Gastric Diseases

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