Distribution of glyphosate-resistant Italian ryegrass (&lt;i&gt;Lolium multiflorum&lt;/i&gt; Lam.) in paddy field margins in Shizuoka prefecture

実, 市原; 義樹, 石田; 清裕, 小池; 由貴, 新實; 揚一, 木田; 径明, 神谷; 亨, 鈴木; 千恵子, 済木; 雅幸, 山下; 均, 澤田

doi:10.3719/weed.61.17

Cited by 5 publications

(8 citation statements)

References 4 publications

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“…Ichihara et al . () investigated the distribution of glyphosate‐resistant Italian ryegrass populations in Shizuoka Prefecture and reported that the percentage of resistant populations was the highest, 58.8%, in Chuen district, and 22.1% of populations from the district included more than 50% of resistant plants in the population. Handayani et al .…”

Section: Discussionmentioning

confidence: 99%

Non‐target‐site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum)

Kurata

Niinomi

Shimono

et al. 2018

Weed Biology and Management

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In Shizuoka Prefecture, Japan, glyphosate-resistant Lolium multiflorum is a serious problem on the levees of rice paddies and in wheat fields. The mechanism of resistance of this biotype was analyzed. Based on LD 50 , the resistant population was 2.8-5.0 times more resistant to glyphosate than the susceptible population. The 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene sequence of the resistant biotype did not show a non-synonymous substitution at Pro106, and amplification of the gene was not observed in the resistant biotype. The metabolism and translocation of glyphosate were examined 4 days after application through the direct detection of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) using liquid chromatograph-tandem mass spectrometer (LC-MS/MS). AMPA was not detected in either biotype in glyphosate-treated leaves or the other plant parts. The respective absorption rates of the susceptible and resistant biotypes were 37.90 AE 3.63% and 41.09 AE 3.36%, respectively, which were not significantly different. The resistant biotype retained more glyphosate in a glyphosate-treated leaf (91.36 AE 1.56% of absorbed glyphosate) and less in the untreated parts of shoots (5.90 AE 1.17%) and roots (2.76 AE 0.44%) compared with the susceptible biotype, 79.58 AE 3.73%, 15.77 AE 3.06% and 4.65 AE 0.89%, respectively. The results indicate that the resistance mechanism is neither the acquisition of a metabolic system nor limiting the absorption of glyphosate but limited translocation of the herbicide in the resistant biotype of L. multiflorum in Shizuoka Prefecture.

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Section: Discussionmentioning

confidence: 99%

Non‐target‐site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum)

Kurata

Niinomi

Shimono

et al. 2018

Weed Biology and Management

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“…L. multiflorum probably developed resistance to glyphosate and glufosinate due to the repeated use of these herbicides. In a population of L. multiflorum found in rice paddy levees (34.78°N, 137.75°E) located about 2 km from the Japanese pear orchards, the survival rates of plants treated with glyphosate-potassium at 2.70 kg ai ha -1 was 3.2% (Ichihara et al 2016). The resistance genes of L. multiflorum can be widely dispersed via pollen movement (Busi et al 2008, Ichihara et al 2016).…”

Section: Levels Of Resistance Of L Multiflorum To Glyphosate and Glumentioning

confidence: 99%

“…In a population of L. multiflorum found in rice paddy levees (34.78°N, 137.75°E) located about 2 km from the Japanese pear orchards, the survival rates of plants treated with glyphosate-potassium at 2.70 kg ai ha -1 was 3.2% (Ichihara et al 2016). The resistance genes of L. multiflorum can be widely dispersed via pollen movement (Busi et al 2008, Ichihara et al 2016). However, the Japanese pear orchards are surrounded by residential areas, vegetable fields, and trees; therefore, the populations in rice paddy levees and Japanese pear orchards are unlikely to be cross-pollinated.…”

Section: Levels Of Resistance Of L Multiflorum To Glyphosate and Glumentioning

confidence: 99%

“…To date, Japanese pear orchards where glyphosate-and glufosinate-resistant L. multiflorum grow have only been found in certain parts of Hamamatsu City. L. multiflorum is an anemophilous, self-sterile, cross-pollinated species, and the resistance genes may be widely dispersed by pollen movement (Busi et al 2008, Ichihara et al 2016. From now on, it will be very important to monitor the distribution of resistant L. multiflorum and the occurrence of resistance in all surrounding areas.…”

Section: Efficacy Of Several Foliar-applied Herbicidesmentioning

confidence: 99%

“…in a vegetable farm and oil palm nursery in Malaysia (Jalaludin et al 2015), L. multiflorum in hazelnut orchards in Oregon, USA (Avila-Garcia & Mallory-Smith 2011), L. multiflorum in vineyards and pear orchards in California, USA (Karn et al 2018), and L. multiflorum and Lolium perenne in vineyards in New Zealand (Ghanizadeh et al 2015). In Japan, resistance to glyphosate (Ichihara et al 2016, Niinomi et al 2013) and glufosinate (Ichihara et al 2018) and multiple resistance to these herbicides (Ichihara et al 2018) have been confirmed in L. multiflorum populations in the levees of rice paddies in Shizuoka Prefecture. These herbicides have also been generally used in orchards in Japan.…”

Section: Introductionmentioning

confidence: 99%

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Emergence of Glyphosate- and Glufosinate-resistant Italian Ryegrass (<i>Lolium multiflorum</i>) Populations in Japanese Pear Orchards in Japan and their Responses to Several Foliar-applied Herbicides

Ichihara

Tominaga

Yamashita

et al. 2020

JARQ

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In this study, we aimed to evaluate the levels of resistance of Lolium multiflorum populations to glyphosate and glufosinate in Japanese pear orchards in Hamamatsu City, Shizuoka Prefecture, Japan, and determine the efficacy of several foliar-applied herbicides to control this weed during several growth stages. Bioassays using glyphosate-potassium (1.08-2.70 kg ai ha -1 ) and glufosinate (0.60-1.00 kg ai ha -1 ) were conducted, and the survival rates of L. multiflorum populations after foliar application were 11.1%-53.3% and 0.0%-5.6%, respectively. After treatments with several foliarapplied herbicides in the early growth stage (at a plant length of about 5 cm) with the maximum dosage, the survival rate of plants treated with glyphosate-potassium (38.9%) was not significantly different from that of plants receiving no treatment (100%). However, the survival rates of plants treated with quizalofop-ethyl (0.0%), glufosinate (17.8%), and glufosinate-P-sodium (18.9%) were significantly lower than that of plants receiving no treatment. In the middle growth stage (at a plant length of about 35 cm), although the survival rate of plants treated with quizalofop-ethyl (8.3%) was significantly lower than that of plants receiving no treatment (100%), the survival rates of other plants were not significantly different from that of plants receiving no treatment. These results indicate that L. multiflorum has developed resistance to glyphosate and glufosinate in the orchards, and suggest that the foliar application of quizalofop-ethyl (0.74 kg ai ha -1 ) is particularly effective for the control of this weed.

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Influence of weed management measures on glyphosate resistance and endophyte infection in naturalized Italian ryegrass (Lolium multiflorum)

Handayani

Tanno

Yamashita

et al. 2017

Weed Biology and Management

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Recently, glyphosate‐resistant Italian ryegrass (Lolium multiflorum Lam.) was found on rice paddy levees in a western region of Shizuoka Prefecture, Japan. Naturalized populations of Italian ryegrass are frequently infected with fungal Epichloë endophytes. Endophytes often confer benefits to their host grasses. This study investigated the influence of five weed management treatments on glyphosate resistance and endophyte infection in Italian ryegrass that was growing on paddy levees where glyphosate‐resistant individuals were dominant. The weed management treatments were: (i) mowing once before the grass flowered; (ii) mowing once during flowering; (iii) mowing twice during flowering; (iv) glyphosate application before flowering; and (v) no treatment. The seeds were collected from the treatment plots in 2013 and 2014. The seeds were examined for endophyte infection and the seedlings that had been grown from the seeds were tested for the frequency of glyphosate resistance. The seedlings that had been derived from the glyphosate treatment showed higher frequencies of glyphosate resistance than those seedlings that had been derived from all the other treatments. Endophytes were found in all populations of the seeds from the paddy levees, with higher infection rates in the seeds that had been derived from the glyphosate treatment and the twice‐mowed treatment. There was a significant relationship between the endophyte infection frequency in the seeds and glyphosate resistance in the seedlings that had been grown from the same populations. The results indicate that where glyphosate herbicides are frequently used, selection for glyphosate‐resistant Italian ryegrass occurs, and along with this, the frequency of endophyte infection also increases.

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Distribution of glyphosate-resistant Italian ryegrass (<i>Lolium multiflorum</i> Lam.) in paddy field margins in Shizuoka prefecture

Cited by 5 publications

References 4 publications

Non‐target‐site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum)

Non‐target‐site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum)

Emergence of Glyphosate- and Glufosinate-resistant Italian Ryegrass (<i>Lolium multiflorum</i>) Populations in Japanese Pear Orchards in Japan and their Responses to Several Foliar-applied Herbicides

Influence of weed management measures on glyphosate resistance and endophyte infection in naturalized Italian ryegrass (Lolium multiflorum)

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