Distribution of gastrin and CCK-like peptides in rat brain

Lorén, Ingemar; Alumets, J.; Håkanson, R.; Sundler, F.

doi:10.1007/bf00689607

Cited by 268 publications

(31 citation statements)

References 9 publications

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“…A few positive cells and a rich network of thick positive fibers were present in the nucleus parabrachialis dorsalis; positive fibers were present in the nuclei dorsalis and ventralis lemniscus lateralis and in the nucleus raphe dorsalis and raphe magnus. A group of large positive cell bodies was present in the caudal part of the nucleus raphe dorsalis ( Some differences between our results and other ipxnunohistochemical studies (9)(10)(11)(12)(13)(14) can be explained by methodological variations. In this work, colchicine treatment, "Bouin Hollande Sublime" perfusion, high sensitivity of the peroxidase-antiperoxidase complex technique, and use of a highly diluted serum in order to achieve minimal background may explain our results.…”

Section: Methodscontrasting

confidence: 80%

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Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat

Vanderhaeghen¹,

Lotstra²,

Mey³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

358

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ABSIRACT The distribution of gastrin-cholecystokinin-like peptide(s) is reported in brain and hypophysis of the rat. The unlabeled peroxidase-antiperoxidase complex immunohistochemical technique was used. Controls of specificity for various peptides were studied with solid-phase absorption. Colchicine treatment was necessary to obtain positivity in many neuronal cell bodies. In addition to their already known distribution, gastrin-cholecystokinins containing neural cell bodies and fibers were present in olfactory structures, in various preoptic and hypothalamic nuclei (except in mamillary bodies), in mesencephalic nucleus linearis rostralis, and in A-10, A-9, and A-8 regions of Dahlstrom and Fuxe, which include substantia nigra. From previous investigations and the present distribution study, it can be inferred that, although most of the brain material consists of cholecystokinin, gastrins may also be present in hypothalamo-posthypophyseal magnocellular cells, in nucleus tractus solitarii, and in the dorsal horn of the spinal cord. The distribution of positive cell bodies in the peripheral part of the paraventricular nucleus and in the dorsal part of the supraoptic nuclei in the hypothalamus is similar to that of oxytocin neurons.

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Section: Methodscontrasting

confidence: 80%

“…In addition, positive cells have also been demonstrated in the paraventricular (12,13), supraoptic (12), and circularis (13) hypothalamic magnocellular nuclei, in the hypothalamic dorsomedial nucleus (14), and in some brain stem nuclei (12,14). Positive fibers have been shown, too, in posterior hypophysis (12,13), spinal cord (11,13), and spinal ganglia (11).…”

mentioning

confidence: 99%

Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat

Vanderhaeghen¹,

Lotstra²,

Mey³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

358

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“…Possible 'gliasomal' contamination of our nerve ending preparations should not contribute significantly to the released peptide inasmuch as CCK has not been detected in glial cells (Loren et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Cholecystokinin release mediated by 5‐HT₃ receptors in rat cerebral cortex and nucleus accumbens

Paudice

Raiteri

1991

British J Pharmacology

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1 The effects of 5-hydroxytryptamine (5-HT) on the release of cholecystokinin-like immunoreactivity (CCK-LI) were examined in synaptosomes prepared from rat cerebral cortex and nucleus accumbens and depolarized by superfusion with 15 mM KCl. 2 In both areas 5-HT, tested between 0.1 and 100 nm, increased the calcium-dependent, depolarizationevoked CCK-LI release in a concentration-related manner. The concentration-response curves did not differ significantly between the two brain areas (EC50: 0.4 + 0.045 nm and 0.48 + 0.053 nm, respectively, in cortical and n. accumbens synaptosomes; maximal effect: about 60% at 10 nm 5-HT).3 The 5-HT,/5-HT2 receptor antagonist methiothepin (300 nM) did not affect the CCK-LI release elicited by 10nm 5-HT. However, the effects of 10nm 5-HT were antagonized in a concentration-dependent manner by the 5-HT3 receptor antagonists (3a-tropanyl)-lH-indole-3-carboxylic acid ester 0.1-100 nM; IC50: 3.56 + 0.42 nm in the cortex and 3.90 + 0.50 nm in the n. accumbens) and ondasetron (IC50: 8.15 + 0.73nm in the cerebral cortex). 5-HT (10nM) was also strongly antagonized by l00nM laH, 3a5aH-tropan-3-yl-3,5-dichlorobenzoate (MDL 72222) another blocker of the 5-HT3 receptor. Moreover, the 5-HT3 receptor agonist 1-phenylbiguanide (tested in the cerebral cortex between 0.1 and 100nM) enhanced CCK-LI release in a manner almost identical to that of 5-HT (EC5o = 0.64 + 0.071 nM).4 It is concluded that 5-HT can act as a potent releaser of CCK-LI in rat cerebrocortex and nucleus accumbens through the activation of receptors of the 5-HT3 type situated on the CCK-releasing terminals. This interaction may provide a rationale for the clinical development of both 5-HT3 and CCK receptor antagonists as novel anxiolytic drugs.

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“…Each ofthese brain-stem regions is known to project to the cerebellum (Olson and Fuxe, 1971;Taber-Pierce et al, 1977;Somana and Walberg, 1979;Gould, 1980;Sato et al, 1983a, b;Haines et al, 1984). Populations of enkephalin- (Hiikfelt et al, 1977;Williams and Dockray, 1983;King et al, 1986a, b), CCK- (Innis et al, 1979;Loren et al, 1979;Vanderhaeghen et al, 1980) 5-HT- (Steinbusch, 1981; or norepinephrine- (Dahlstrom and Fuxe, 1964) containing neurons have also been found within one or more of these CRFcontaining regions. It is reasonable, therefore, to hypothesize dual transmitter-containing mossy fiber or other afferent-type projections from such brain-stem locations to the cerebellum.…”

Section: Distribution Of Crf Immunoreactivity Within Thejlocculus Andmentioning

confidence: 99%

Corticotropin-releasing factor in cerebellar afferent systems: a combined immunohistochemistry and retrograde transport study

1988

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The flocculus and paraflocculus of cat and sheep cerebellum were studied with immunohistochemical methods, using antisera to corticotropin-releasing factor (CRF). CRF immunoreactivity was present within 3 populations of varicose nerve fibers. One population of CRF- immunoreactive (CRF-IR) fibers appeared to appose Purkinje cell somata and to follow their dendrites into the molecular layer. This arrangement suggested they were climbing fibers. A second group of CRF- IR profiles reminiscent of mossy fibers was widely distributed throughout the granule cell layer. A third population of CRF-IR fibers was present as a beaded plexus lying parallel to the pial surface, above and subadjacent to the Purkinje cell layer. The fibers of this plexus extended into the Purkinje cell layer and surrounded these somata. The source of some of the CRF-IR fibers within the flocculus and paraflocculus was determined by a retrograde axonal transport study utilizing the fluorescent tracer Fast blue (FB) in combination with the immunohistochemical localization of CRF. It was determined that CRF-IR perikarya within the inferior olivary nucleus gave rise to a population of climbing fibers within those lobules. Furthermore, all divisions of the inferior olive were found to contain CRF-IR somata. This latter finding suggests the potential for CRF-IR climbing fiber projections from the inferior olive to other regions of the cerebellar cortex. The existence of CRF-IR mossy fibers and fibers within the ganglionic plexus suggests the possibility of CRF-IR afferent projections from other regions of the brain stem to the flocculus and paraflocculus.

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Distribution of gastrin and CCK-like peptides in rat brain

Cited by 268 publications

References 9 publications

Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat

Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat

Cholecystokinin release mediated by 5‐HT₃ receptors in rat cerebral cortex and nucleus accumbens

Corticotropin-releasing factor in cerebellar afferent systems: a combined immunohistochemistry and retrograde transport study

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Distribution of gastrin and CCK-like peptides in rat brain

Cited by 268 publications

References 9 publications

Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat

Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat

Cholecystokinin release mediated by 5‐HT3 receptors in rat cerebral cortex and nucleus accumbens

Corticotropin-releasing factor in cerebellar afferent systems: a combined immunohistochemistry and retrograde transport study

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Cholecystokinin release mediated by 5‐HT₃ receptors in rat cerebral cortex and nucleus accumbens