Distribution of fibroblastic colony-forming cells in rabbit bone marrow and assay of their osteogenic potential by anin vivo diffusion chamber method

Ashton, Brian A.; Eaglesom, C. C.; I, Bab; Owen, Maureen

doi:10.1007/bf02405298

Cited by 88 publications

(25 citation statements)

References 14 publications

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“…bone marrow-derived bone precursor cells were not hematogenous in origin, as these cells did not express the pan-hematopoietic cell surface antigen CD34 and failed to respond to hematopoietic growth factors (9). Moreover, unlike the murine system, human bone marrow-initiated bone cell cultures cannot be derived from bone marrow stromal cells (9 The relationship between bone marrow-derived human osteoprogenitor cells and past reports on bone formation derived from the colony-forming unit fibroblast (CFU-f) (29)(30)(31) (12,34). Pragmatically, this requires that investigations into the control of a given cellular lineage use purified progenitor cell populations, serum-free culture conditions, and purified (e. g., recombinant) growth factors.…”

Section: Resultsmentioning

confidence: 99%

Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors.

Long¹,

Jeyashakila²,

Ashcraft³

et al. 1995

J. Clin. Invest.

216

145

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Human bone marrow contains a distinct cell population that expresses bone proteins and responds to transforming growth factor p1 (TGF-,B), but not to hematopoietic growth factors (Long, M. W., J. L. Williams, and K. G. Mann. 1990. J. Clin. Invest. 86:1387-1395. We now report the isolation, characterization, and growth factor responsiveness of these precursors to human osteoblasts and the identification of a human osteoprogenitor cell. Immunological separation of human bone marrow nonadherent low-density (NALD) cells results in a marked enrichment of cells that express osteocalcin, osteonectin, and bone alkaline phosphatase. Flow cytometric analyses show that distinct cell subpopulations exist among these isolated cells. The majority of the bone antigen-positive cells are approximately the size of a lymphocyte, whereas other, less frequent antibody-separated subpopulations consist of osteoblast-like cells and osteoprogenitor cells. In serum-free cultures, TGF-.8 stimulates the small, antigen-positive cells to become osteoblastlike, as these cells both increase in size, and express increased levels of osteocalcin and alkaline phosphatase. Antibody-separated cells also contain a separate population of clonal progenitor cells that form colonies of osteoblast-like cells when cultured in serum-free, semi-solid media. Two types of human osteoprogenitor cells are observed: a colonyforming cell (CFC) that generates several hundred bone antigen-positive cells, and a more mature cluster-forming cell that has a lesser proliferative potential and thus generates clusters of 20-50 antigen-positive cells. Osteopoietic colony-forming cells and cluster-forming cells have an obligate but differential requirement for osteogenic growth factors. The CFCs respond to TGF-,B, basic fibroblast growth factor (bFGF), bone morphogenic protein-2 (BMP-2), and 1, 25-dihydroxy vitamin D3 (1,25-OH D3). In contrast to the colony-forming cells, cluster-forming cells are regulated predominately by 1,25-OH D3 and TGF-fi, but fail to respond to bFGF. We conclude that human bone marrow Clin. Invest. 1995. 95:881-887.)

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Section: Resultsmentioning

confidence: 99%

Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors.

Long¹,

Jeyashakila²,

Ashcraft³

et al. 1995

J. Clin. Invest.

216

145

View full text Add to dashboard Cite

show abstract

“…These investigators further identified an osteogenic cell phenotype in a subpopulation of adherent bone marrow fibroblasts, localized near the (endosteal) bone surface (40,42). These cells are the progeny of fibroblast colony-forming cells (CFC-F) which are serumresponsive, generate heterogeneous populations of cells, and are strongly adherent (43)(44)(45)(46).…”

Section: Resultsmentioning

confidence: 99%

Expression of human bone-related proteins in the hematopoietic microenvironment.

Long¹,

Williams²,

Mann³

1990

J. Clin. Invest.

111

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Given the intimate relationship between bone and bone marrow, we hypothesized that the human bone marrow may function as a source (or reservoir) of bone-forming progenitor cells. We observed a population of cells within the bone marrow which produce bone-specific or bone-related proteins. The production of these proteins was developmentally regulated in human long-term bone marrow cell cultures; the bone proteinproducing cells (BPPC) are observed under serum-free, shortterm culture conditions, respond to bone-related and not hematopoietic growth factors, and are derived from a population of low-density, nonadherent, MylO-negative (or low MylO density), marrow cells (MylO is an antigen found on most hematopoietic progenitor cells). Cultivation of marrow-derived BPPC in secondary, serum-containing cultures results in their differentiation into osteoblastlike cells. At this stage of development, BPPC produce an extracellular matrix which incorporates both bone-related proteins and radiolabeled calcium. Human bone marrow BPPC thus represent a newly described cell phenotype important to both bone and hematopoietic cell biology. (J. Clin. Invest. 1990Invest. . 86:1387Invest. -1395

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“…It is also possible that there are several sources of calcifying vascular cells in the arterial wall just as in bone where colony-forming unit fibroblasts (CFU-f) [3,4,29] as well as osteoblasts [23] participate in bone formation. Studies on the nature of the developmental lineages leading to the formation of bone cell types in humans, namely the osteoblast, osteoclast and CFU-f, suggest that osteoclasts may be the progeny of macrophages/monocytes [2,15,23] whereas osteoblast and CFU-f arise in some unknown manner from local mesenchymal cells and, as such, they do not express the panhaematopoietic cell surface antigen CD34 and fail to respond to haematopoietic growth factors [27,28].…”

Section: Discussionmentioning

confidence: 99%

Detection of Vascular Dendritic Cells and Extracellular Calcium-Binding Protein S-100 in Foci of Calcification in Human Arteries.

Bobryshev

Lord

1995

Acta Histochem. Cytochem

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Distribution of fibroblastic colony-forming cells in rabbit bone marrow and assay of their osteogenic potential by anin vivo diffusion chamber method

Cited by 88 publications

References 14 publications

Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors.

Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors.

Expression of human bone-related proteins in the hematopoietic microenvironment.

Detection of Vascular Dendritic Cells and Extracellular Calcium-Binding Protein S-100 in Foci of Calcification in Human Arteries.

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