Distribution of ExPEC Virulence Factors, blaCTX-M, fosA3, and mcr-1 in Escherichia coli Isolated From Commercialized Chicken Carcasses

Cyoia, Paula Signolfi; Koga, Vanessa Lumi; Nishio, Erick Kenji; Houle, Sébastien; Dozois, Charles M.; Brito, Kelly Cristina Tagliari de; Brito, Benito Guimarães de; Nakazato, Gerson; Kobayashi, Renata Katsuko Takayama

doi:10.3389/fmicb.2018.03254

Cited by 47 publications

(42 citation statements)

References 49 publications

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“…ESBL-producing E. coli showed higher health risks related to hospital-acquired infections compared to non-ESBL-producing isolates. 51 CTX-M β-lactamases are the most widespread types of ESBLs, which have been identified in the mid-2000s in clinical E. coli isolates. 52 In this study, 96.6% of ESBL genes were classified as bla CTX-M .…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Escherichia coli Isolated from Chinese Patients During 2002–2017

Tian¹,

Zheng²,

Sun³

et al. 2020

IDR

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Background: The emergence and spread of carbapenem-resistant Escherichia coli (E. coli) pose a serious threat to human health worldwide. This study aimed to investigate the molecular mechanisms underlying carbapenem resistance and their prevalence among E. coli in China. Methods: A collection of 5796 E. coli clinical isolates were collected from the First Affiliated Hospital of Wenzhou Medical University from 2002 to 2017. Sensitivity to antibiotics was determined using the agar dilution method. The detection of carbapenemases production and the prevalence of resistance-associated genes were investigated through modified carbapenem inactivation method (mCIM), PCR and sequencing. The mutations in outer membrane porins genes (ompC and ompF) were also analyzed by PCR and sequencing assays. The effect of efflux pump mechanism on carbapenem resistance was also tested. E. coli were typed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results: A total of 58 strains (1.0%) of carbapenem-resistant E. coli were identified. The strains carrying bla KPC-2 and bla NDM accounted for 22.4% (13/58) and 51.7% (30/58), respectively. Among bla NDM -positive strains, 27 bla NDM genes were assigned to bla NDM-5 , while the remaining three strains were bla NDM-1 , whereas bla VIM , bla IMP , bla OXA-48 , and bla SHV were not found. The CTX-M-type β-lactamase genes accounted for 96.6% (56/58). In addition, bla TEM-1 genes were identified in 58.6% of tested strains. In carbapenem-resistant isolates, mutations in OmpC (the majority of mutated sites were D192G and Q104_F141del, accounting for 54.5%) and OmpF (large deletions S75_V127del, W83_D135del and Q88_D135del) were detected. Of note, the antibiotic resistance was not associated with overexpression of efflux pump. Moreover, MLST categorized the 58 carbapenem-resistant isolates into 19 different sequence types. PFGE analysis revealed that homology among the carbapenem-resistant isolates was low and sporadic. Conclusion: The bla NDM was the principal resistance mechanism of carbapenem-resistant E. coli in the hospital. bla NDM-5 is becoming a new threat to public health and the alteration of outer membrane porins might help further increase the MIC of carbapenem.

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Escherichia coli Isolated from Chinese Patients During 2002–2017

Tian¹,

Zheng²,

Sun³

et al. 2020

IDR

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“…Studies indicate that these genes are mainly carried by E. coli and nontyphoid Salmonella, but also by Escherichia fergusonii, Klebsiella pneumoniae, Citrobacter diversus, and Proteus mirabilis [136][137][138][139][140]. Reports include animals on farms [36,[136][137][138][141][142][143][144][145][146][147], whole carcasses, or meat pieces available in Brazilian retail markets [69,136,138,140,145,148,149], and exported chicken meat [150][151][152]. The same genes were detected in turkeys with clinical signs of colibacillosis [153].…”

Section: Streptococcus Sppmentioning

confidence: 99%

“…Regarding food samples, mcr-1 was detected in E. coli obtained from chicken meat samples acquired in retail markets in São Paulo State (SE), and from three of 409 chicken carcasses produced in the three Southern states, most of them in isolates carrying β-lactamase genes [149,169]. The gene has also been reported in S. Typhimurium, and S. Schwarkergund collected from pork meat, and poultry meat acquired in the Rio Grande do Sul (S) and São Paulo (SE) States, respectively [95,170].…”

Section: Colistin Resistance-mcrmentioning

confidence: 99%

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Rabello

Bonelli

Penna

et al. 2020

Animals

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In animal husbandry, antimicrobial agents have been administered as supplements to increase production over the last 60 years. Large-scale animal production has increased the importance of antibiotic management because it may favor the evolution of antimicrobial resistance and select resistant strains. Brazil is a significant producer and exporter of animal-derived food. Although Brazil is still preparing a national surveillance plan, several changes in legislation and timely programs have been implemented. Thus, Brazilian data on antimicrobial resistance in bacteria associated with animals come from official programs and the scientific community. This review aims to update and discuss the available Brazilian data on this topic, emphasizing legal aspects, incidence, and genetics of the resistance reported by studies published since 2009, focusing on farm animals and derived foods with the most global public health impact. Studies are related to poultry, cattle, and pigs, and mainly concentrate on non-typhoid Salmonella, Escherichia coli, and Staphylococcus aureus. We also describe legal aspects of antimicrobial use in this context; and the current occurrence of genetic elements associated with resistance to beta-lactams, colistin, and fluoroquinolones, among other antimicrobial agents. Data here presented may be useful to provide a better understanding of the Brazilian status on antimicrobial resistance related to farm animals and animal-derived food products.

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“…The location of mcr-1 on a plasmid means that it is more easily replicated and can spread horizontally between bacteria, leading to pan-resistant or even superbug strains. Since their initial discovery in China, mcr-1 –containing plasmids with various Inc-types have been discovered in gram-negative bacteria worldwide ( Malhotra-Kumar et al., 2016 ; Li et al., 2017 ; Trung et al., 2017 ; Cyoia et al., 2018 ; Brilhante et al., 2019 ; Vounba et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of avian-origin mcr-1–positive Escherichia coli with a potential risk to humans in Tai'an, China

Lin

et al. 2020

Poultry Science

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Multidrug-resistant ( MDR ) Escherichia coli are responsible for difficult-to-treat infections. We sought to determine the prevalence and characteristics of MDR E. coli strains isolated from poultry and clinical patients in the same geographical region. Eighty-seven E. coli strains were isolated from poultry with perihepatitis lesions at different slaughterhouses, and 356 nonrepetitive E. coli strains were isolated from clinical patients. All samples were continuously collected from October to December 2017 in Tai'an, China. The presence of the mcr-1 gene in the strains was assessed by PCR. The genetic relationships of the polymyxin ( POL )-resistant E. coli strains were analyzed by pulsed-field gel electrophoresis and multilocus sequence typing. The results indicate that the POL resistance rate for the E. coli isolates from poultry was 31.03% (27 of 87), whereas the human-origin E. coli isolates were 100% sensitive to POL. The mcr-1 gene and extended-spectrum β-lactamase bla CTX-M-14 genes were identified in all 27 POL-resistant avian-origin E. coli isolates. Our pulsed-field gel electrophoresis analysis suggested that the 27 strains were represented by 14 pulsotypes, among which there were 3 strains each with A, E, I, and K pulsotypes, and 1 to 2 strains represented by the other 10 pulsotypes. Furthermore, multilocus sequence typing molecular typing identified 16 sequence types, including 4 ST156 strains, 3 ST533 strains, and 1 to 2 strains represented by the remaining 14 sequence types. In summary, the E. coli strains isolated in the Tai'an area all showed the MDR phenotype, the rate of which for poultry was higher than that for humans. No POL-resistant human-origin E. coli strains were identified in the clinical patients. Our study reveals that poultry-derived MDR mcr-1 –positive E. coli strains may pose a potential risk to humans, and the surveillance findings presented herein will be conducive to our understanding of the prevalence and characteristics of mcr-1 –positive E. coli strains in the Tai'an area.

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Distribution of ExPEC Virulence Factors, blaCTX-M, fosA3, and mcr-1 in Escherichia coli Isolated From Commercialized Chicken Carcasses

Cited by 47 publications

References 49 publications

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Prevalence of avian-origin mcr-1–positive Escherichia coli with a potential risk to humans in Tai'an, China

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