Distribution of exogenous heavy metals in the hepatocytes of calves: A morphometric study

Horký, Drahomír; Lauschová, Irena; Illek, J.; Pechová, Anna; Šindelář, Miroslav

doi:10.1002/jemt.10044

Cited by 2 publications

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“…In the liver as well as the kidneys of bank voles exposed for 6 weeks to dietary Cd (40-80 lg Cd/g dry weight), Cd was mainly found in the cytosol but also in the nuclei and particulate fractions (Wlostowski & Krasowska 1999). In calves injected with 1-10 mg Cd/animal/day for 95 days, Cd in liver cells was detected in the nuclei, the mitochondria, the endoplasmic reticulum and the lysosomes (Horky et al 2002). Also, a significant amount of Cd was recovered in the nuclei of rat liver as soon as 1 h after a single injection of 3 lmol Cd/Kg (Rau et al 1987).…”

Section: Discussionmentioning

confidence: 96%

Kinetics of the early subcellular distribution of cadmium in rat hepatocytes

2005

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The kinetics of the early subcellular distribution of cadmium (Cd) was characterized in primary cultures of rat hepatocytes exposed to 10, 50 and 100 microM Cd in a serum-free WME medium for 10, 30 or 60 min. Our results demonstrate a time- and concentration-dependent increase in Cd content with the highest metal concentration measured in the cytosol, whereas the lowest was observed in the mitochondria. With the exception of early localization in the plasma membrane, Cd concentrations in fractions were characterized by the following decreasing order of magnitude: cytosol > low density molecules approximately nuclei > lysosomes approximately mitochondria. We also found evidence for: (i) a two-step process for Cd distribution in the nuclei and mitochondria; and (ii) a time-dependent 'slow' process of transfer from the plasma membrane to the cytosol. Saturation in Cd uptake was observed at 50 microM in most cell fractions at 10 and 30 min, except for the plasma membrane. The lack of apparent saturation for Cd accumulation at 60 min was not related to an increase in metallothionein synthesis. Altogether, our data provide insights into the dynamics of transfer between intracellular compartments, and allow a better identification of the organelles that are the most subjected to Cd toxicity for early exposure conditions.

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Section: Discussionmentioning

confidence: 96%

Kinetics of the early subcellular distribution of cadmium in rat hepatocytes

2005

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“…However, they did not use a histochemical assessment of cadmium and, thus, on the basis of our results, we can confirm their findings only in part. The morphometry of hepatocytes in relation to lysosome distribution is reported in another study (Horký et al, 1997).…”

Section: Electron Microscopymentioning

confidence: 90%

Histochemical and ultrahistochemical localization of heavy metals in calf organs

Horký

Illek

Pechová

2002

Microscopy Res & Technique

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Cadmium, zinc, selenium, and copper were administered, singly or in combination, orally or subcutaneously. Experiment I included 32 calves of both sexes; six received Cd (two groups), Zn, Cd, and Zn, and Cd and Se (two groups) and one group was a control. In Experiment II (21 bulls), three were given Cd, Cd, and Cu, and Cd and Zn, respectively, and one group was a control. For light microscopy, in Experiment I the highest amounts of silver granules were present in the samples of liver, small intestine, and vesicular gland of all the exposed groups; in Experiment II the most affected organs were liver, kidney, and small intestine. For electron microscopy, in Experiment I, after administration of Cd and Zn, the highest amounts of granules were seen in the cytoplasm of hepatocytes and cells of the proximal and distal renal tubules and the lowest amounts were found in glandular cells of the pancreas. Administration of Cd and Se resulted in the presence of large numbers of granules in the nuclei and nucleoli of spermatogonies. In Experiment II, ingestion of Cd and Zn in feed led to the appearance of highest amounts of granules in the nucleoli, nuclei, and cytoplasm of cells in testes, kidneys, and pancreas. Following Cd intake, the highest accumulation of granules was observed in the nucleoli of hepatocytes and cells of the proximal and distal renal tubules. Combined Cd and Cu produced the highest number of granules in cells of the proximal and distal renal tubules and in the nucleoli and nuclei of germinal epithelium.

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Distribution of exogenous heavy metals in the hepatocytes of calves: A morphometric study

Cited by 2 publications

References 25 publications

Kinetics of the early subcellular distribution of cadmium in rat hepatocytes

Kinetics of the early subcellular distribution of cadmium in rat hepatocytes

Histochemical and ultrahistochemical localization of heavy metals in calf organs

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