Distribution of estrogen receptors in the rat pituitary as studied by in situ hybridization

Gene expression of steroid hormone receptors in brain and peripheral tissues relative to reproduction.

“…8B) 3) Discussion The in situ hybridization findings reported here demonstrated selective distribution of ERmRNA in the central hypothalamus, amygdala and uterus. The sites of ERmRNA-containing neurons were largely in agreement with those of ER in the previous autoradiographical (40) and immunohistochemical (48) studies, and with the in situ hybridization ones (42,43,50). Discrepancy exists, however, on the existence of ERmRNA in the amygdala between ours and Pelletier's (42).…”

“…Our preliminary results have revealed the existence of ERmRNA even in poorly differentiated cancers in which ERs are absent, implying different regulation of the ER level in the cancer lacking in hormone dependency. Several in situ hybridization studies have reported the distribution of ERmRNA containing neurons in the rat brain (42,43,50), which is roughly similar as that of ER (40,48).…”

Effects of ovariectomy and chronic estradiol administration on pituitary–thyroid axis in adult rats

“…However, we detected a minor decrease in TSH cell functioning. Since estrogen receptors were detected in pituitary thyrotrophs (Pelletier et al, 1988;Stefaneanu et al, 1994), we assumed that estradiol amplifies the negative feedback mechanism of thyroid hormones on TSH cells, thus contributing to the preserved level of serum TSH just below the control values. This view is supported by the results of in vitro studies that were obtained by Ahlquist et al (1987) who showed that estrogen made TSH cells more sensitive to thyroid hormone regulation (which resulted in a lower TSH mRNA level).…”

Oestrogen receptor protein and mRNA in adenocarcinoma of the uterine cervix

“…In situ hybridisation A 24 base cDNA oligonucleotide probe (5' CTC CAG CTC GTT CCC TTG GAT CTG 3') complementary to human (Green et al, 1986;Pelletier et al, 1988;Ponglikitmongkol et al, 1988;Graham et al, 1991). The probe was labelled at the 5' end with digoxigenin-l 1-UTP and purified by HPLC.…”

“…The staining was intensified by immersing the slides for 10 min in 0.5% CuSO4 in 0.85% NaCl. Counterstaining was performed with 1% methylgreen.In situ hybridisation A 24 base cDNA oligonucleotide probe (5' CTC CAG CTC GTT CCC TTG GAT CTG 3') complementary to human ER mRNA coding for amino acids 17-24 was synthesised to our specifications (British Bio-technology Ltd., Abingdon, (Green et al, 1986;Pelletier et al, 1988;Ponglikitmongkol et al, 1988;Graham et al, 1991). The probe was labelled at the 5' end with digoxigenin-l 1-UTP and purified by HPLC.…”