Distribution of epithelia and glands of the nasal septum mucosa in the rat

Katz, Sima Godosevicius; Merzel, J

doi:10.1159/000144835

Cited by 42 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S5 C and D). In the VNO, the PAS stain identifies the vomeronasal gland, also termed Jacobson's gland (25). In VNO sections from LRRC26 KO animals, the magenta signal of PAS staining colocalized with the dark blue signals from the β-gal reaction (SI Appendix, Fig.…”

Section: Lrr26 Protein Can Be Readily Detected In Most Native Tissuesmentioning

confidence: 99%

Knockout of the LRRC26 subunit reveals a primary role of LRRC26-containing BK channels in secretory epithelial cells

Yang

González-Pérez

Mukaibo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Leucine-rich-repeat-containing protein 26 (LRRC26) is the regulatory γ1 subunit of Ca 2+ -and voltage-dependent BK-type K + channels. BK channels that contain LRRC26 subunits are active near normal resting potentials even without Ca 2+ , suggesting they play unique physiological roles, likely limited to very specific cell types and cellular functions. By using Lrrc26 KO mice with a β-gal reporter, Lrrc26 promoter activity is found in secretory epithelial cells, especially acinar epithelial cells in lacrimal and salivary glands, and also goblet and Paneth cells in intestine and colon, although absent from neurons. We establish the presence of LRRC26 protein in eight secretory tissues or tissues with significant secretory epithelium and show that LRRC26 protein coassembles with the pore-forming BK α-subunit in at least three tissues: lacrimal gland, parotid gland, and colon. In lacrimal, parotid, and submandibular gland acinar cells, LRRC26 KO shifts BK gating to be like α-subunit-only BK channels. Finally, LRRC26 KO mimics the effect of SLO1/BK KO in reducing [K + ] in saliva. LRRC26-containing BK channels are competent to contribute to resting K + efflux at normal cell membrane potentials with resting cytosolic Ca 2+ concentrations and likely play a critical physiological role in supporting normal secretory function in all secretory epithelial cells.L arge-conductance, voltage-and Ca 2+ -regulated BK-type channels are widely expressed proteins, found not only in excitable cells, such as neurons, muscle, and endocrine cells, but also nonexcitable cells, including salivary (1) and lacrimal gland (2) acinar cells, and colonic crypt cells (3). Given the almost ubiquitous expression of BK channels among cells that play quite distinct physiological roles, it is particularly important to define the specific properties of BK channels in a given cell type and determine what the specific physiological role played by BK channels in a given cell may be. A hallmark of BK channels is their dual regulation by both membrane voltage and cytosolic Ca 2+ (4), both properties embedded within the tetramer of pore-forming α-subunits of each BK channel (5). However, the specific range of voltages over which a BK channel is active at a given Ca 2+ concentration is markedly dependent on the identity of regulatory subunits that can coassemble with the α-subunit in the mature channel complex. Of the two families of known BK regulatory subunits, β (6-11) and γ (12-14), an important feature of many of these subunits is the ability to shift the range of activation voltages at a given Ca 2+ . Although there is growing information about the loci of expression and functional roles of BK channels containing specific β-subunits (15), much less is known about those BK channels containing the γ1 (LRRC26, leucine-rich-repeat-containing subunit 26) subunit. However, LRRC26 is particularly fascinating because it causes the largest shift in BK gating (approximately −120 mV) of any known non-pore-forming regulatory subunit, resulting in BK channels that...

show abstract

Section: Lrr26 Protein Can Be Readily Detected In Most Native Tissuesmentioning

confidence: 99%

Knockout of the LRRC26 subunit reveals a primary role of LRRC26-containing BK channels in secretory epithelial cells

Yang

González-Pérez

Mukaibo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…However, JG are positive to PAS reaction in all three species. In humans, the dorsal acini of the BG (DBG) are negative to PAS reaction (Katz & Merzel, 1977), but most acini are PAS positive in the ventral BG (VBG) and in all JG. Consequently, there seems to be some degree of regional specialization in the secretion of JG and BG, with differences between species.…”

Section: Introductionmentioning

confidence: 99%

Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat

Chichet¹,

Genovese²,

Bielli³

2007

Int. J. Morphol.

View full text Add to dashboard Cite

SUMMARY:The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO -is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with a semiquantitative system (0, 1, 2 or 3 crosses) in JG, ventral Bowman glands (VBG), dorsal Bowman glands (DBG), sensitive chemoreceptory neuroepithelium (Ne), receptor-free epithelium (RFE) and nasal septum respiratory epithelium (RE) histological slides of male rats at 5, 15, 25 and 35 days of age. The VBG and JG were intensely positive to PAS reaction since 5 days of age, and weakly positive to Alcyan blue reaction (AB) since 15 days of age, but not at 5 days of age. Both JG and VBG were strongly positive to androgen receptor immunolocalization at all ages studied (15, 25 and 35 days of age). DBG were always negative to both PAS and AB reactions. The NE and the RFE and nasal mucosa luminar epithelium were negative. To our knowledge, this is the first report of androgen receptor (AR) immunolocalization in the vomeronasal organ and the nasal septum mucosa. The present results also suggest an influence of androgens on the mechanism of pheromones contacting with the Ne of the VNO through regulation of the Jacobson glands secretion.

show abstract

“…Approximately 50% of the nasal passages is lined by sensory olfactory neuroepithelium, 46% by the respiratory and transitional epithelium, and 3.5À4% by squamous epithelium. The septal olfactory organ (Organ of Masera), an isolated patch of olfactory epithelium surrounded by respiratory epithelium at the base of the nasal septum near the entrance to the nasopharyngeal duct, occupies nearly 2% of the surface area (Bojsen-Møller, 1975;Katz and Merzel, 1977;Weiler and Farbman, 2003). In addition to these epithelial types, the mucosa lining the nasal passages shows highly specific regional features with respect to physiology and biochemistry.…”

Section: Nasal Cavitymentioning

confidence: 99%

“…The lamina propria in the respiratory epithelial region is a highly vascularized, loose, fibroelastic connective tissue that contains many mucous and serous glands (Bojsen-Møller, 1964;Katz and Merzel, 1977;Uraih and Maronpot, 1990). The glands in the anterior half of the nasal septum (anterior septal glands) are tubuloalveolar glands comprising numerous solitary acini and a single long duct that empties into the vestibule.…”

Section: Nasal Cavitymentioning

confidence: 99%