Distribution of entomopathogenic nematodes in Southern Cameroon

Kanga, Françoise Nicole Ngo; Waeyenberge, Lieven; Häuser, S.; Moens, Maurice

doi:10.1016/j.jip.2011.09.008

Cited by 42 publications

(22 citation statements)

References 30 publications

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“…cinerea , khanii , stackebrandtii [8]. H. baujardi has been described in Vietnam [20], Brazil [21] and Cameroon [22], and in Brazil was found to carry P. luminescens [23].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Xenorhabdus and Photorhabdus spp. and Their Symbiotic Entomopathogenic Nematodes from Thailand

et al. 2012

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Xenorhabdus and Photorhabdus spp. are bacterial symbionts of entomopathogenic nematodes (EPNs). In this study, we isolated and characterized Xenorhabdus and Photorhabdus spp. from across Thailand together with their associated nematode symbionts, and characterized their phylogenetic diversity. EPNs were isolated from soil samples using a Galleria-baiting technique. Bacteria from EPNs were cultured and genotyped based on recA sequence. The nematodes were identified based on sequences of 28S rDNA and internal transcribed spacer regions. A total of 795 soil samples were collected from 159 sites in 13 provinces across Thailand. A total of 126 EPNs isolated from samples taken from 10 provinces were positive for Xenorhabdus (n = 69) or Photorhabdus spp. (n = 57). Phylogenetic analysis separated the 69 Xenorhabdus isolates into 4 groups. Groups 1, 2 and 3 consisting of 52, 13 and 1 isolates related to X. stockiae, and group 4 consisting of 3 isolates related to X. miraniensis. The EPN host for isolates related to X. stockiae was S. websteri, and for X. miraniensis was S. khoisanae. The Photorhabdus species were identified as P. luminescens (n = 56) and P. asymbiotica (n = 1). Phylogenenic analysis divided P. luminescens into five groups. Groups 1 and 2 consisted of 45 and 8 isolates defined as subspecies hainanensis and akhurstii, respectively. One isolate was related to hainanensis and akhurstii, two isolates were related to laumondii, and one isolate was the pathogenic species P. asymbiotica subsp. australis. H. indica was the major EPN host for Photorhabdus. This study reveals the genetic diversity of Xenorhabdus and Photorhabdus spp. and describes new associations between EPNs and their bacterial symbionts in Thailand.

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“…cinerea , khanii , stackebrandtii [8]. H. baujardi has been described in Vietnam [20], Brazil [21] and Cameroon [22], and in Brazil was found to carry P. luminescens [23].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Xenorhabdus and Photorhabdus spp. and Their Symbiotic Entomopathogenic Nematodes from Thailand

et al. 2012

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“…For example, a soil pH of 10 or higher is likely to be detrimental to EPN applications, whereas a range of 4-8, is not likely to have any significant effect (Kung, Gaugler, & Kaya, 1990a); thus the pH of most agricultural soils are unlikely to have an impact on EPN survival. Nonetheless, soil pH has been reported to affect native EPN distributions (Kanga, Waeyenberge, Hauser, & Moens, 2012). Soil texture can have a substantial effect on nematode movement and survival (Barbercheck, 1992;Kaya, 1990).…”

Section: Abiotic Factorsmentioning

confidence: 99%

Entomopathogenic Nematode Application Technology

Shapiro‐Ilan

Dolinski

2015

Nematode Pathogenesis of Insects and Other Pests

106

151

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“…Although African EPN surveys have included Egypt (Glazer, Galper, & Sharon, 1991;Shamseldean, Abou El-Sooud, Abd-Elgawad, & Saleh, 1996), Kenya (Burnell & Stock, 2000), Ethiopia (Mekete, Gaugler, Nguyen, Mandefro, & Tessera, 2005), Tanzania (Mwaitulo, Haukeland, Saethre, Laudisoit, & Maerere, 2011), South Africa (Malan, Knoetze, & Moore, 2011), Cameroon (Kanga, Waeyenberge, Hauser, & Moens, 2012) and Nigeria (Akyazi, Ansari, Ahmed, Crow, & Mekete, 2012), the number of African surveys has been small when compared with the other continents (with the exception of Antarctica). As far as the authors are aware, there is no information on the occurrence and diversity of EPNs in Rwanda.…”

Section: Introductionmentioning

confidence: 99%

New entomopathogenic nematodes from semi-natural and small-holder farming habitats of Rwanda

Yan¹,

Waweru

Qiu³

et al. 2016

Biocontrol Science and Technology

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Five field surveys for indigenous entomopathogenic nematodes (EPNs) were conducted in 22 semi-natural and 17 small-holder farming habitats across 16 districts of different altitudes in the northern, eastern, southern and Kigali city provinces of Rwanda. In 2014, 216 mixed soil samples were collected and subsamples thereof baited with Galleria mellonella or Tenebrio molitor larvae. Five samples from five locations and habitats were positive for nematodes (2.8%). Nine nematode species/strains were isolated and five successfully maintained. DNA sequence comparisons and morphological examinations revealed Steinernema carpocapsae, Heterorhabditis bacteriophora, as well as two steinernematids and one heterorhabditid with no species designation. The isolates (strains) were named Steinernema sp. RW14-M-C2a-3, Steinernema sp. RW14-M-C2b-1, Steinernema carpocapsae RW14-G-R3a-2, H. bacteriophora RW14-N-C4a and Heterorhabditis sp. RW14-K-Ca. These are the first records of naturally occurring EPNs in Rwanda. It is also the first record of S. carpocapsae from Africa. Finding H. bacteriophora from tropical rather than temperate Africa was surprising. The found nematodes will serve as the basis for efficacy screening, and for mass production in a biocontrol agent factory at Rubona Research Centre of the Rwanda Agriculture Board with the ultimate aim of delivering effective, safe and environmentally benign pest control for soil-inhabiting pests. ARTICLE HISTORY

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Distribution of entomopathogenic nematodes in Southern Cameroon

Cited by 42 publications

References 30 publications

Diversity of Xenorhabdus and Photorhabdus spp. and Their Symbiotic Entomopathogenic Nematodes from Thailand

Diversity of Xenorhabdus and Photorhabdus spp. and Their Symbiotic Entomopathogenic Nematodes from Thailand

Entomopathogenic Nematode Application Technology

New entomopathogenic nematodes from semi-natural and small-holder farming habitats of Rwanda

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