Distribution of eight serotypes of Ureaplasma urealyticum in cases of non-gonococcal urethritis and of gonorrhoea, and in healthy persons.

Piot, Peter

doi:10.1136/sti.52.4.266

Cited by 24 publications

(21 citation statements)

References 13 publications

(7 reference statements)

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“…However, no agreement in association of particular serovars with diseases was achieved among the different patient groups, including the invasive isolates recovered from usually sterile sites. Previous studies also showed no consistent data correlating individual serovars and pathogenic outcome, even among invasive isolates (10,12,29,33,46,50,58,59). This suggests that serovar designation is not a reliable subspecies marker for determining the differential pathogenicity of Ureaplasma.…”

Section: Discussionmentioning

confidence: 96%

“…Although several studies reported that U. urealyticum is more pathogenic than U. parvum (1,13,26,30,34,36), conflicting results have been found by others (6,18), so it is possible that differential pathogenicity might exist at the serovar level rather than at the species level. Inconsistent results implicating specific serovars (or serovar groups) with various clinical conditions (10,12,29,33,46,50,58) coupled with frequent detection of Ureaplasma isolates comprised of more than one serovar have also been reported (10,17,29,32,50). Differing results among investigations could be related to inadequate or imprecise typing methods.…”

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confidence: 98%

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Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

et al. 2011

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Ureaplasma parvum and Ureaplasma urealyticum are sexually transmitted, opportunistic pathogens of the human urogenital tract. There are 14 known serovars distributed between the two species. For decades, it has been postulated based upon limited data that virulence is related to serotype specificity. The results were often inconclusive due to the small sample size and extensive cross-reactivity between certain serovars. We developed real-time quantitative PCRs that allow reliable differentiation of the two species and type strains of each of the 14 serovars. To investigate species and serovar distributions, we typed 1,061 clinical isolates of human ureaplasmas from diverse patient populations. There was only a tenuous association between individual Ureaplasma serovars and certain patient populations. This may in part be explained by the fact that almost 40% of the isolates were genetic mosaics, apparently arising from the recombination of multiple serovars. This explains the extensive cross-reactivity based upon serotyping and the lack of consistent association of given serotypes with disease.

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Section: Discussionmentioning

confidence: 96%

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confidence: 98%

Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

et al. 2011

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“…Several studies showed no consistent predominance of any Ureaplasma serotype in men with nongonococcal urethritis (NGU) or differences from serotypes detected in asymptomatic controls while others have found serotype 4 to occur more commonly in this condition (57,218,259,270). Lin (173) found no differences in the serotype distribution in normal college women, women with salpingitis, and pregnant women who delivered normal, low-birth-weight, or stillborn infants.…”

Section: Other Infectionsmentioning

confidence: 99%

Mycoplasmas and Ureaplasmas as Neonatal Pathogens

Katz²,

2005

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SUMMARY The genital mycoplasmas represent a complex and unique group of microorganisms that have been associated with a wide array of infectious diseases in adults and infants. The lack of conclusive knowledge regarding the pathogenic potential of Mycoplasma and Ureaplasma spp. in many conditions is due to a general unfamiliarity of physicians and microbiology laboratories with their fastidious growth requirements, leading to difficulty in their detection; their high prevalence in healthy persons; the poor design of research studies attempting to base association with disease on the mere presence of the organisms in the lower urogenital tract; the failure to consider multifactorial aspects of diseases; and considering these genital mycoplasmas only as a last resort. The situation is now changing because of a greater appreciation of the genital mycoplasmas as perinatal pathogens and improvements in laboratory detection, particularly with regard to the development of powerful molecular nucleic acid amplification tests. This review summarizes the epidemiology of genital mycoplasmas as causes of neonatal infections and premature birth; evidence linking ureaplasmas with bronchopulmonary dysplasia; recent changes in the taxonomy of the genus Ureaplasma; the neonatal host response to mycoplasma and ureaplasma infections; advances in laboratory detection, including molecular methods; and therapeutic considerations for treatment of systemic diseases.

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“…The pathogenic potential of these organisms has been widely disputed, and the significance of their isolation is often unclear (4, 17, 18). The possibility that some strains or biotypes of U. urealyticurn are pathogenic and others are not has been suggested (15), but serological differences have not been clearly associated with pathogenicity (10,15). Deoxyribonucleic acid hybridization (5) and one-dimensional (8, 13) and two-dimensional (9) polyacrylamide gel electrophoretic analyses of [3'S]methionine-labeled proteins have revealed differences among strains of U .…”

mentioning

confidence: 99%

“…In some cases, a GEL-CODE silver staining kit (UpJohn Diagnostics, Kalamazoo, Mich.) was used. isoelectric focussing in the first dimension followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in 10 to 20% gradient gels in the second dimension. The major medium components are indicated (spots A through D).…”

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confidence: 99%

Specific Protein Differences Among Strains of Ureaplasma urealyticum as Determined by Two-Dimensional Gel Electrophoresis and a Sensitive Silver Stain

Swenson¹,

Hamont²,

Dunbar³

1983

International Journal of Systematic Bacteriology

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The proteins of nine strains of Ureaplasrna urealyticum, representing serovars I through IX, were analyzed by using two-dimensional gel electrophoresis and a sensitive silver staining procedure. The electrophoretic patterns of the nine strains tested could be divided into two distinct major groups, one group consisting of serovars 11, IV, V, VII, VIII, and IX (group A) and one group consisting of serovars I, 111, and VI (group B).Ureaplasma urealyticum strains are a serologically heterogeneous group of organisms (16) that are frequently found in human genital tracts. The pathogenic potential of these organisms has been widely disputed, and the significance of their isolation is often unclear (4, 17, 18). The possibility that some strains or biotypes of U. urealyticurn are pathogenic and others are not has been suggested (15), but serological differences have not been clearly associated with pathogenicity (10,15). Deoxyribonucleic acid hybridization (5) and one-dimensional (8, 13) and two-dimensional (9) polyacrylamide gel electrophoretic analyses of [3'S]methionine-labeled proteins have revealed differences among strains of U . urealyticum. In two of these studies (5, 9), the nine recognized serovars could be separated into two major groups. In this report we describe an additional method of analyzing the proteins of U. urealyticurn strains by using two-dimensional electrophoresis and a sensitive silver-based stain which combines with proteins in a fashion that produces reproducible and characteristic colors (12). Our findings confirm previous reports of two groups within the species U. urealyticurn.Eight strains of U . urealyticum originally obtained from F. T. Black, Aarhus, Denmark, and designated serovars I through VIII (3) and one strain obtained from J. A. Robertson, Alberta, Canada, and designated serovar IX (11) were used. These organisms were grown in U-9 broth (14) supplemented with 2% PPLO serum fraction (Difco Laboratories, Detroit, Mich.) at 37°C in an atmosphere containing 5% C02. Cultures in the log phase of growth were harvested by centrifugation at 12,000 x g for 1 h and washed three times with phosphate-buffered saline. The cell pellet obtained from 1 liter of a 20-h culture was sufficient for more than six analyses. Uninoculated medium was incubated and alkalinized to the same degree as growing cultures and was then centrifuged and processed in the same manner. Each strain was grown and tested at least three times.Each washed cell pellet was suspended in six times its volume of sample buffer containing 2% sodium dodecyl sulfate, 2% P-mercaptoethanol, and 1% cyclohexylaminoethanesulfonic acid (Calbiochem-Behring Corp., La Jolla, Calif.) (pH 9.5) and heated at 98°C for 10 min. Electrophoresis in the first dimension (isoelectric focusing) and the second dimension (sodium dodecyl sulfate slab gel electrophoresis) was performed by using the method of Anderson et al. (2), as described by Dunbar et al. (6). Cylindrical isoelectric focusing gels containing ampholines with a wide pH range (pH 3.5 to 10...

show abstract

Distribution of eight serotypes of Ureaplasma urealyticum in cases of non-gonococcal urethritis and of gonorrhoea, and in healthy persons.

Cited by 24 publications

References 13 publications

Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

Mycoplasmas and Ureaplasmas as Neonatal Pathogens

Specific Protein Differences Among Strains of Ureaplasma urealyticum as Determined by Two-Dimensional Gel Electrophoresis and a Sensitive Silver Stain

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