Distribution of EGFR, BMP-2, and p53 in kidney of healthy newborn, adult and old highland-plateau yaks

Wang, Ting; Cui, Yan; Liu, Penggang; He, Junlin; Zhang, Qian

doi:10.5603/fm.a2018.0067

Cited by 1 publication

(1 citation statement)

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“…These salivary glands were capable of regeneration. When salivary glands undergo duct ligation, the acinar cells atrophy and lose their function, but when duct ligation is released, the atrophied acinar cells recover to their original state [32]. Previous studies have reported that ΔNp63, a p63 isoform [19], and epiregulin [33], which show high expression after duct ligation compared to nonduct ligation, are factors involved in regeneration and recovery.…”

Section: Discussionmentioning

confidence: 99%

Acinar Cell Proliferation Promoted by BMP2 in Injured Mouse Parotid Gland: BMP2 Promotes Cell Proliferation in Parotid Gland

Yokoyama

Katsumata-Kato

Fujita-Yoshigaki

2023

International Journal of Dentistry

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Objective. To identify factors that affect salivary gland recovery, we investigated the expression and function of bone morphogenetic protein 2 (BMP2) in mice. Materials and Methods. Using a micro clip, mice parotid glands were removed 7 days after the ligation of the unilateral parotid excretory duct. Thereafter, they were weighed and stained with hematoxylin and eosin, and BMP2 expression was examined via real-time reverse transcription-polymerase chain reaction. Primary cultures of parotid glands were prepared, and BMP2 protein was added to the culture medium for 48 hr to examine its effect on cell proliferation. E-cadherin and vimentin expression was examined using western blotting. Finally, immunohistochemical staining using an anti-Ki67 antibody was performed. Results. Duct-ligated parotid glands weighed less than those that were collected after sham surgery and showed acinar cell atrophy. They also showed higher BMP2 expression than control glands. Primary-cultured parotid acinar cells supplemented with BMP2 showed higher proliferative potential than control cells. Furthermore, they showed E-cadherin, but not vimentin, expression, and their percentage of Ki67-positive cells were higher than that corresponding to the controls. Conclusions. Injury to salivary glands by excretory duct ligation increased BMP2 expression, which may be involved in maintaining salivary gland function by inducing acinar cell proliferation.

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Section: Discussionmentioning

confidence: 99%