Distribution of dfrA1 and cat1 antibiotic resistance genes in uropathogenic Escherichia coli isolated from teens pregnant women in Iraq

Abstract

The present study aims to detect the distribution of dfrA1 and cat1 antibiotic resistance genes among uropathogenic Escherichia coli (UPEC) in pregnant teen women and determine their susceptibility to common antibiotic uses. We collected urine (116) samples from patients in hospitals in Baghdad, Iraq. Isolation and identification of bacteria (culturing, biochemical test, and genetically by 16S rRNA gene), antibiotic susceptibility tests (eight antibiotics), and detection of the dfrA1 and cat1 resistance genes, and used SPSS program for statistically analyzing the results. The distributed UPEC in patients most than another causative agent in percentage (50%). It was highly resistant to Trimethoprim (82%) and Cefotaxime (82%) antibiotics. And they highly distributed frequency for dfrA1-gene (Trimethoprim resistance gene) (74%) than cat1-gene (Chloramphenicol resistance gene) (38%).

Synergistic Antimicrobial Activity of Eugenol in Combination with Fosfomycin to Combat Escherichia coli and Potential Effect on Plasmid‐Mediated Fosfomycin Resistance Genes

“…Gram‐negative aerobic bacteria can colonize the urogenital mucosa through the use of fimbriae, pili, adhesin, and P1‐blood group phenotypic receptors. Numerous virulence factors, both secreted and surface‐associated, contribute to the ability of E. coli isolates to cause UTIs, which explains their high prevalence [10] …”

“…Numerous virulence factors, both secreted and surface-associated, contribute to the ability of E. coli isolates to cause UTIs, which explains their high prevalence. [10] Molecular techniques based on PCR have emerged as an alternate way for reliable identification and classification of bacterial species where the targeted gene for amplification is the 16S rRNA. [11] One of the most essential factors in this categorization is the stability and conservation of the 16S rRNA gene across time.…”

The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population

“…Genotype and allele frequencies have been presented as percentage frequencies after using Hardy-Weinberg equilibrium (HWE), and differences have been assessed by Pearson's Chisquare test. The association between TP53 SNPs and AML was detected as an odds ratio (OR) with a confidence interval (CI) estimate of 95% 15,16 .…”

Identification and Characterization of a Bacteriophage with Lytic Activity against Multidrug Resistant E. coli

“…Antibiotic-resistant E. coli is one of the biggest problems threatening people around the world and becoming resistant to almost all antibiotics, especially among Iraqi patients. For that, finding a new safe alternative is necessary and phage therapy is one of the promising alternatives [26][27][28][29]. Different phages produce different plaques sizes and shapes that are useful in phage characterization.…”