Distribution of cphA or related carbapenemase-encoding genes and production of carbapenemase activity in members of the genus Aeromonas

Rossolini, G. M.; Zanchi, Alessandra; Chiesurin, Alessandra; Amicosante, Gianfranco; Satta, G.; Guglielmetti, P

doi:10.1128/aac.39.2.346

Cited by 51 publications

(38 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cphA-related genes were noted in A. veronii and A. hydrophila, but not in A. caviae. Such results were concordant CphA in bacteraemic Aeromonas isolates with those in previous studies (Balsalobre et al, 2009;Rossolini et al, 1995). However, it is a novel finding that the cphA-related genes were present in all A. aquariorum isolates.…”

Section: Discussionsupporting

confidence: 76%

“…The only exception, A. aquariorum A2-155, was susceptible to imipenem even when tested with a large inoculum. Rossolini et al (1995) found that 20 % of cphA hybridized-positive A. hydrophila and A. veronii bv. sobria isolates were unable to express MBL activity, and they proposed that genetic modification had led to silencing of cphA in these species.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with other class B enzymes, CphA MBL has a very specific substrate profile, being active on penem and carbapenem only, and not on penicillin and cephalosporins (Segatore et al, 1993). The distribution of cphA-related genes is species-related: they are mainly found in isolates of A. hydrophila, A. veronii and Aeromonas jandaei, but not in A. caviae (Balsalobre et al, 2009;Rossolini et al, 1995). However, CphA MBL production is not easily detected by in vitro susceptibility testing unless using large inocula.…”

Section: Introductionmentioning

confidence: 99%

“…Tested by the agar dilution method with a larger inoculum (10 8 c.f.u. ), the imipenem MICs for Aeromonas strains with cphA-related genes were ¢4 mg ml 21 , higher than the susceptible breakpoint, in a study by Rossolini et al (1995).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Distribution and phenotypic and genotypic detection of a metallo-β-lactamase, CphA, among bacteraemic Aeromonas isolates

Chen

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

The objectives of the study were to investigate the distribution of cphA-related genes (cphA) encoding a CphA metallo-b-lactamase (MBL) among 51 consecutive Aeromonas blood isolates and to compare different phenotypic methods for detecting CphA. The presence of cphA was detected by PCR. Four phenotypic methods, the imipenem-EDTA combined disc test, imipenem-EDTA MBL Etest, agar dilution test and modified Hodge test (MHT), were used to detect imipenem susceptibility and MBL production. The results showed that 35 (69 %) blood isolates had cphA. All (100 %) of 16 Aeromonas aquariorum isolates and 12 Aeromonas veronii isolates, and 4 (80 %) of 5 Aeromonas hydrophila isolates, carried cphA, but none of 15 Aeromonas caviae isolates did. With the standard inocula, irrespective of the presence or absence of cphA, all but one (50, 98 %) isolates were susceptible to imipenem tested by disc diffusion, Etest and agar dilution (10 4 c.f.u. spot inocula), and did not exhibit MBL production by the imipenem-EDTA combined disc test and MBL Etest. By the agar dilution test using large inocula (10 7 c.f.u.), 34 (97 %) of 35 cphA + isolates had imipenem MICs of ¢16 mg ml "1 , higher than the susceptible breakpoint (4 mg ml "1 ), and demonstrated positive results for the MHT, while one cphA + and all 17 cphA " isolates had imipenem MICs of ¡4 mg ml "1 . In conclusion, the distribution of cphA among aeromonads is species-specific, found in A. aquariorum, A. veronii and A. hydrophila, and the MHT may be a phenotypic screening test for CphA production.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distribution and phenotypic and genotypic detection of a metallo-β-lactamase, CphA, among bacteraemic Aeromonas isolates

Chen

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Electroporation of E. coli DH5␣ was performed with a Gene-Pulser apparatus (Bio-Rad Laboratories, Richmond, Calif.), according to the instructions of the manufacturer. Southern hybridization was carried out directly on the dried gels, as described previously (34), with DNA probes labeled with 32 P by the random-priming technique. The bla CTX-M probe was made by use of a 1:1 mixture of amplicons generated with primers CTX-MU1 and CTX-MU2 from bla CTX-M-2 and bla CTX-M-15 , respectively.…”

Section: Methodsmentioning

confidence: 99%

Detection of CTX-M-Type β-Lactamase Genes in Fecal Escherichia coli Isolates from Healthy Children in Bolivia and Peru

Pallecchi

Malossi

Mantella

et al. 2004

Antimicrob Agents Chemother

View full text Add to dashboard Cite

A survey was carried out from August to November 2002 to evaluate the antimicrobial susceptibilities of fecal Escherichia coli isolates from 3,208 healthy children from four different urban areas of Latin America, two in Bolivia (Camiri and Villa Montes) and two in Peru (Yurimaguas and Moyobamba). Ceftriaxone-resistant E. coli isolates were detected in four children, one from each of the areas sampled. The isolates exhibited a multidrug-resistant phenotype, including resistance to oxyimino-cephalosporins and aztreonam, and the MICs of ceftazidime for the isolates were lower than those of cefotaxime. By PCR and sequencing, the bla CTX-M-2 determinant was detected in three isolates and the bla CTX-M-15 determinant was detected in one isolate (from Peru). The CTX-M-2-producing isolates belonged to three different phylogenetic groups (groups A, B2, and D), while the CTX-M-15-producing isolate belonged to phylogenetic group D. The bla CTX-M-2 determinants were transferable to E. coli by conjugation, while conjugative transfer of the bla CTX-M-15 determinant was not detectable. Plasmids harboring the bla CTX-M-2 determinant exhibited similar restriction profiles, and in all of them the gene was located on a 2.2-kb PstI fragment, suggesting a genetic environment similar to that present in In35 and InS21. The findings of the present study confirm the widespread distribution of CTX-M-type ␤-lactamases and underscore the role that commensal E. coli isolates could play as a potential reservoir of these clinically relevant resistance determinants. This is the first report of CTX-M-type enzymes in Bolivia and Peru and also the first report of the detection of CTX-M-15 in Latin America.

show abstract

Which antibiotics and breakpoints should be used for Aeromonas susceptibility testing? Considerations from a comparison of agar dilution and disk diffusion methods using Enterobacteriaceae breakpoints

Lamy

Laurent²,

Kodjo

et al. 2012

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

Aeromonas species are environmental organisms that are responsible for numerous infections in humans and animals. Their antimicrobial susceptibility is usually evaluated using Enterobacteriaceae breakpoints. Although disk diffusion and minimum inhibitory concentration (MIC)-based methods are important for infectious disease management and epidemiological surveys of resistance, comparisons between these two methods have not been extensively studied for Aeromonas isolates. We propose the first extensive comparison of agar dilution and disk diffusion susceptibility testing methods, performed for 20 antimicrobial agents, including unevaluated or incompletely evaluated antibiotics (ticarcillin with or without clavulanic acid, ertapenem, tigecycline), on 146 Aeromonas isolates affiliated with six Aeromonas species via molecular means. We evaluated the level of agreement between Enterobacteriaceae breakpoints-based methods. Reliable agreement (>95%) was observed for piperacillin, cefotaxime, cefepime, nalidixic acid, ofloxacin, ciprofloxacin, gentamicin, amikacin, tetracycline and cotrimoxazole, whereas marked inconsistencies between the methods were noted for carbapenems, amoxicillin-clavulanic acid, ticarcillin, ticarcillin-clavulanic acid, tobramycin and tigecycline. The results indicate that beta-lactam and aminoglycoside susceptibility testing should be limited to piperacillin, cephems, gentamicin and amikacin. Co-amoxiclav should be avoided given the lack of agreement between the two methods. Adjusting the zone diameter breakpoints for tigecycline and cefoxitin could also improve the agreement to >95% and reduce the error rates to acceptable levels.

show abstract

Distribution of cphA or related carbapenemase-encoding genes and production of carbapenemase activity in members of the genus Aeromonas

Cited by 51 publications

References 21 publications

Distribution and phenotypic and genotypic detection of a metallo-β-lactamase, CphA, among bacteraemic Aeromonas isolates

Distribution and phenotypic and genotypic detection of a metallo-β-lactamase, CphA, among bacteraemic Aeromonas isolates

Detection of CTX-M-Type β-Lactamase Genes in Fecal Escherichia coli Isolates from Healthy Children in Bolivia and Peru

Which antibiotics and breakpoints should be used for Aeromonas susceptibility testing? Considerations from a comparison of agar dilution and disk diffusion methods using Enterobacteriaceae breakpoints

Contact Info

Product

Resources

About