Distribution of collagens type I, type III and type V in the pancreas of rat, dog, pig and man

Abstract

The presence of collagens type I, type III and type V was determined immunohistochemically in pancreatic tissue of rat, pig, dog and man. The reaction to anti-collagen type I is weak (pig, dog) or moderate (rat, man) in the peri-insular region and in the lobar, lobular and acinar septa, whereas the reaction to anti-collagen type III is well developed. In rat and dog, the latter reaction deposit on the lobar and acinar septa is prominent. These elements only show a moderate reaction intensity in pig and man. The peri-insular region displays a weak (rat, dog, man) or very weak (pig) reaction against collagen type III. Anti-collagen type V reacts moderately (rat, dog, man) or weakly (pig) in the lobar and lobular septa. The acinar septa show a moderate (rat, dog, man) or very weak (pig) reaction. This information regarding the types and distribution of the collagenous compounds in pancreatic extracellular matrix could lead to differentiated enzymatic pancreas dissociation and, ultimately, increased islet yield and improved reproducibility of pancreatic islet isolation procedures for transplantation purposes.

Blockade of β1 Integrin–Laminin-5 Interaction Affects Spreading and Insulin Secretion of Rat β-Cells Attached on Extracellular Matrix

“…Other studies have shown that basement membrane structure and major extracellular matrix proteins are not found between islet cells (11,38,39). Within the islet, basement membrane was observed only around capillaries (21).…”

“…A continuous periinsular extracellular matrix that contains laminin, fibronectin, and collagen types IV and V has been described in human pancreas (21,38). Other studies have shown that basement membrane structure and major extracellular matrix proteins are not found between islet cells (11,38,39).…”

Blood vessels of human islets of Langerhans are surrounded by a double basement membrane

“…Some findings have indicated that in the human pancreas, endocrine cells are separated from exocrine cells by reticulin fibres [15] and the endocrine-exocrine interface in different species variably appears to contain collagens I, IV, V and VI, as well as Lm [16][17][18]. Electron microscopy detected [19] a typical BM facing the endocrine cell at the endocrine-exocrine interface as a peri-islet BM, but the same study failed to detect BM inside human islets.…”

Microencapsulation and tissue engineering as an alternative treatment of diabetes

“…Donor variables include age, gender, body mass index, diet, trauma, hypoxia or ischemic conditions (11). Pancreatic architecture, composition of Clostridium histolyticum collagenase preparations, endotoxicity, digestion time and cold ischemic time are some of the variables related to the islet purification procedure (12).…”

“…ECM is found in two forms, namely, interstitial matrix and basement membrane. The pancreatic islet basement membrane is composed of collagen IV, laminin and fibronectin (12). Disruption of islet cell-matrix interactions (30-32) and loss of the basement membrane lead to induction of apoptosis (33,34).…”