Distribution of Collagen Types I, III, and V in Pregnant Mouse Endometrium

Spiess, Karin; Teodoro, W. R.; Zorn, Telma Maria Tenório

doi:10.1080/03008200601166194

Cited by 44 publications

(38 citation statements)

References 36 publications

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“…These results agree with those of another study in rat uterus that shows 30% and 55% clearance of COL I and COL III, respectively, in the implantation site from Day 5.5 to Day 6.5; and 16% and 31% clearance of COL I and COL III, respectively, in the implantation site from Day 6.5 to Day 7.5 [6]. Previous studies in the mouse indicate weaker COL III staining on Day 5 than on later days [7], with reorganization into sparsely distributed, thick intercellular fibrils by Day 7 [39]. The expression pattern of COL IV differs from that of the other three collagen types.…”

Section: Discussionsupporting

confidence: 92%

“…It has been demonstrated that different collagen types have distinct spatiotemporal expression patterns during implantation. The major fibril-forming COL I, III, and V are all reduced in implantation sites compared to non-implantation sites in the rat uterus [4], mainly because they progressively disappear from the primary and secondary decidual zones from Day 5.5 to Day 7.5 (Day 0 as mating night) [5][6][7]. Their expression patterns in the outer nondecidualized stroma and myometrial tissues do not seem to change during this period [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…In the embryo implantation phase of early pregnancy, progressive differentiation of fibroblastlike stromal cells into hypertrophic polygonal decidual cells correlates with profound alterations in collagen gene expression [4][5][6][7]. It has been demonstrated that different collagen types have distinct spatiotemporal expression patterns during implantation.…”

Section: Introductionmentioning

confidence: 99%

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Altered Spatiotemporal Expression of Collagen Types I, III, IV, and VI in Lpar3-Deficient Peri-Implantation Mouse Uterus

Diao

Aplin

Xiao

et al. 2010

Biology of Reproduction

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Lpar3 is upregulated in the preimplantation uterus, and deletion of Lpar3 leads to delayed uterine receptivity in mice. Microarray analysis revealed that there was higher expression of Col3a1 and Col6a3 in the Preimplantation Day 3.5 Lpar3(-/-) uterus compared to Day 3.5 wild-type (WT) uterus. Since extracellular matrix (ECM) remodeling is indispensable during embryo implantation, and dynamic spatiotemporal alteration of specific collagen types is part of this process, this study aimed to characterize the expression of four main uterine collagen types: fibril-forming collagen (COL) I and COL III, basement membrane COL IV, and microfibrillar COL VI in the peri-implantation WT and Lpar3(-/-) uterus. An observed delay of COL III and COL VI clearance in the Lpar3(-/-) uterus may be associated with higher preimplantation expression of Col3a1 and Col6a3. There was also delayed clearance of COL I and delayed deposition of COL IV in the decidual zone in the Lpar3(-/-) uterus. These changes were different from the effects of 17beta-estradiol and progesterone on uterine collagen expression in ovariectomized WT uterus, indicating that the altered collagen expression in Lpar3(-/-) uterus is unlikely to be a result of alterations in ovarian hormones. Decreased expression of several genes encoding matrix-degrading metallo- and serine proteinases was observed in the Lpar3(-/-) uterus. These results demonstrate that pathways downstream of LPA3 are involved in the dynamic remodeling of ECM in the peri-implantation uterus.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Altered Spatiotemporal Expression of Collagen Types I, III, IV, and VI in Lpar3-Deficient Peri-Implantation Mouse Uterus

Diao

Aplin

Xiao

et al. 2010

Biology of Reproduction

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“…Collagen content increases up to 8-fold at term compared with the nonpregnant state and undergoes rapid resorption following parturition. Different collagen types have distinct spatiotemporal expression patterns during decidualization and pregnancy in rodents [27,28].…”

Section: Genes Affecting Uterine Morphology Architecture and Tissuementioning

confidence: 99%

Changes in Mouse Uterine Transcriptome in Estrus and Proestrus1

Yip

Suvorov

Connerney

et al. 2013

Biology of Reproduction

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Changes in the CD-1 mouse uterine transcriptome during proestrus and estrus were investigated to help elucidate mechanisms of uterine tissue remodeling during the estrus cycle and their regulation by estrogen and progesterone in preparation of the uterus for pregnancy. Mice were staged beginning at 6 weeks of age, and uterine horns were harvested after monitoring two estrus cycles. Microarray analysis of whole uterine horn RNA identified 2428 genes differentially expressed in estrus compared to proestrus, indicating there is extensive remodeling of mouse uterus during the estrus cycle, affecting ~10% of all protein-encoding genes. Many (~50%) of these genes showed the same differential expression in independent analyses of isolated uterine lumenal epithelial cells. Changes in gene expression associated with structural alterations of the uterus included remodeling of the extracellular matrix, changes in cell keratins and adhesion molecules, activation of mitosis and changes in major histocompatibility complex class II (MHCII) presentation, complement and coagulation cascades, and cytochrome P450 expression. Signaling pathways regulated during the estrus cycle, involving ligand-gated channels, Wnt and hedgehog signaling, and transcription factors with poorly understood roles in reproductive tissues, included several genes and gene networks that have been implicated in pathological states. Many of the molecular pathways and biological functions represented by the genes differentially expressed from proestrus to estrus are also altered during the human menstrual cycle, although not necessarily at the corresponding phases of the cycle. These findings establish a baseline for further studies in the mouse model to dissect mechanisms involved in uterine tissue response to endocrine disruptors and the development of reproductive tract diseases.

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“…In mice, one of the most striking events is the increase of collagen fibers diameter [4][5] . Decidual cells synthesize collagen 6 , and collagen types I, III and V were identified as the major components of thick fibrils [7][8][9] . Members of the small leucine-rich proteoglycans (SLRPs) family are differentially expressed during decidualization.…”

Section: Colágenos Tipo I Iii E Vmentioning

confidence: 99%

Estabelecimento de um modelo de gestação complicada por diabetes tipo 1 em camundongos: avaliação do seu impacto sobre o ambiente uterino no início da gestação.

Favaro¹

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AGRADECIMENTOSÀ minha querida Mãe, Vanda Favaro, muito obrigado pela sua constante preocupação com minha educação e minha vida. Você foi fundamental nessa caminhada. Ensinou-me valores que permeiam minha conduta, e incentivou-me a buscar um futuro melhor por meio da educação. Aos meus irmãos, Mishel Favaro Piveta e Renato Favaro, pelo companheirismo e apoio, vocês foram fundamentais! Aos meus avós Devair Favaro (in memorian) e Emilia Favaro (in memorian), pelo seu exemplo de dedicação à família. A família desempenha um papel essencial na construção da identidade do indivíduo, o suporte de vocês foi essencial para essa conquista. Espero que minha ausência seja compreendida.À Profa. Telma Zorn, minha "Mãe Científica", muito obrigado por me acolher em seu laboratório e em sua vida. Esses anos sob a sua orientação fizeram com que aumentasse ainda mais o amor que sinto pela universidade, educação e pesquisa. A Senhora é meu exemplo de educadora. Todo o conhecimento que me foi transmitido, pelas suas palavras e seu exemplo, constituem os alicerces que fundamentam minhas ações, como futuro pesquisador e educador.Aos integrantes do LBR & MEC, Ambart Covarrubias, amiga chilena e colaboradora; Cleusa Pelegrini, amiga e exemplo de dedicação, com quem tive a oportunidade de conviver e aprender muito sobre histologia, Fernanda Barrence, amiga e importante colaboradora, sua ajuda foi essencial para este estudo, assim como para o bom andamento do laboratório; Fernanda Fernandes, amiga, realizou sua iniciação científica no laboratório; Juliane Sanches, amiga desde a graduação em Biomedicina na UEL e atual colaboradora; Munick Fulquim e Priscila Raspantini, na época, estudantes de obstetrícia e iniciação científica, importantes colaboradoras durante as etapas iniciais do projeto; Renato Salgado, amigo e importante colaborador científico, obrigado pela valiosa ajuda na correção dos artigos e da tese; Prof. Sebastián San Martín, amigo e colaborador. Fui muito privilegiado em poder conviver e contar com o apoio de todos vocês na realização deste estudo. Formamos uma excelente equipe! Cada um, a sua maneira, fez uma contribuição para este estudo, por isso compartilho-o com vocês. By means of this study our laboratory has developed a new mouse model of pregnancy complicated by long-term type 1 diabetes and evaluated the impact of this disease on the uterine environment at early pregnancy. The age of pregnancy studied (168 hours) comprises the stage in which antimesometrial decidua is fully developed and organogenesis is in progress, of particular interest due to higher rates of malformations in diabetic pregnancies. In addition, the myometrium is at the first adaptative phase of pregnancy (proliferative phase) and diabetic pregnancies are However, no differences were observed between groups. Effects of diabetes on the myometrium were investigated in two subgroups: D1 (90-110D) and D2 (100-110D).Histomorphometrical analysis revealed an increase in the distance between muscle layers in D1, indicating edema, and diminution of the distan...

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Distribution of Collagen Types I, III, and V in Pregnant Mouse Endometrium

Cited by 44 publications

References 36 publications

Altered Spatiotemporal Expression of Collagen Types I, III, IV, and VI in Lpar3-Deficient Peri-Implantation Mouse Uterus

Altered Spatiotemporal Expression of Collagen Types I, III, IV, and VI in Lpar3-Deficient Peri-Implantation Mouse Uterus

Changes in Mouse Uterine Transcriptome in Estrus and Proestrus1

Estabelecimento de um modelo de gestação complicada por diabetes tipo 1 em camundongos: avaliação do seu impacto sobre o ambiente uterino no início da gestação.

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