Distribution of chloramphenicol residues in lactating cows following an external application

Pengov, A.; Flajs, Vesna Cerkvenik; Zadnik, T.; Marinšek, J.; Pogačnik, M.

doi:10.1016/j.aca.2004.07.035

Cited by 18 publications

(6 citation statements)

References 8 publications

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“…Neff et al (2006) during a reference study on the prevalence of salmonella in flocks in Switzerland also isolated Salmonella strains. Furthermore, salmonella has been known to be the most prevalent pathogen to cause intramammary infections in poultry leading to major economic losses (Pengov et al, 2005) and…”

Section: Resultsmentioning

confidence: 99%

Abusive use of antibiotics in poultry farming in Cameroon and the public health implications

Wadoum

Zambou

Anyangwe

et al. 2016

British Poultry Science

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The types and methods of use of antibiotics in poultry farms in Cameroon, residual levels and potential microbial resistance were determined. A questionnaire-based survey identified the different antibiotics used and high-performance liquid chromatography (HPLC) was used to determine residual levels of antibiotics. Pathogens were isolated, identified by use of commercial API kits and minimum inhibition concentration (MIC) was determined. Oxytetracyclin, tylocip and TCN (oxytetracycline, chloramphenicol and neomycin) were the most frequently used antibiotics. Antibiotics screened by HPLC were chloramphenicol, tetracycline and vancomycin. All of them except vancomycin were detected, and the concentration of these antibiotics was higher than the maximum residual limits (MRL) set by regulatory authorities. No residues of various antibiotics were found in egg albumen or yolk. The concentration of tetracycline was significantly higher in liver (150 ± 30 µg/g) than in other tissues. Foodborne pathogens, including Salmonella spp., Staphylococcus spp., Listeria spp., Clostridium spp. and Escherichia spp., were identified. Most of the pathogens were resistant to these various antibiotics tested. These findings imply the need for better management of antibiotic use to control sources of food contamination and reduce health risks associated with the presence of residues and the development of resistant pathogens by further legislation and enforcement of regulations on food hygiene and use of antibiotics.

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Section: Resultsmentioning

confidence: 99%

Abusive use of antibiotics in poultry farming in Cameroon and the public health implications

Wadoum

Zambou

Anyangwe

et al. 2016

British Poultry Science

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“…These findings although not the main scope of this study, indicate a possible health risk because S. aureus may produce a heat stable toxin in raw milk [35,36]. Furthermore, S. aureus has been known to be the most prevalent pathogen to cause intramammary infections in dairy ruminants leading to major economic losses [37,38]. However, the S. aureus isolated from the above samples were resistant to both Gentamicin and Streptomycin (Table 3) and this result is quite alarming because if the drugs were or are to be used to treat and control the condition, regular doses may no longer be effective; thus, promoting a high health and residual levels risk on the animals and humans.…”

Section: Microbiological Analysis Of Milk Samplesmentioning

confidence: 97%

Quantification of Antibiotic Residues and Determination of Antimicrobial Resistance Profiles of Microorganisms Isolated from Bovine Milk in Lebanon

Zeina¹,

Pamela²,

Fawwak³

2013

FNS

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The rapid growth of dairy sectors in the Middle East, particularly in Lebanon, led to extensive use of antibiotics to enhance the health and productivity of animals. Prolonged usage may lead to antibiotic residues in foods of animal origin; hence, the emergence of antimicrobial resistant microorganisms. Accurate data on the antibiotic usage in livestock treatment, antibiotic residues and antimicrobial resistances in raw milk in Lebanon are lacking. This study aimed to investigate the types and usages of antibiotics in cattle, their residual levels and the potential microbial resistances in raw milk samples. A questionnaire-based survey identified Gentamicin and Streptomycin as the most frequently used antibiotics. Selected raw milk samples from main dairy farms were then analyzed in duplicate by quantitative ELISA for the antibiotics residual levels. The mean residual levels of Gentamicin and Streptomycin were 90 and 80 μg/L, respectively; which are below the allowable maximum residue limit of 200 μg/L as set by the FAO/WHO. Staphylococcus aureus, Listeria monocytogenes, E. coli and total aerobic microorganisms isolated from the milk samples were then tested for resistance against Gentamicin and Streptomycin by the disc agar diffusion method. All the S. aureus, E. coli, and L. monocytogenes isolates showed high resistance to Gentamicin. However, 95% of S. aureus, 60% of E. coli and 58% of L. monocytogenes isolates were resistant to Streptomycin. The obtained results provide evidence that antimicrobial resistant strains of the above pathogens have become remarkably widespread in raw milk. This requires better management for antibiotic usages among livestock farmers to control sources of food contamination and reduce the health risks associated with the development of resistant microbial strains.

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“…Qualitative and quantitative analyses of CAP have been performed using rapid test kits (radio-immunoassays and enzyme immunoassays) ( , ), thin layer chromatography (), gas chromatography (GC) with an electron capture detector after chemical derivatization ( , ), immunoaffinity chromatography (), or high-performance liquid chromatography (HPLC) with ultraviolet detection ( , ). All of these methods suffer from low sensitivity and are considered unsuitable for the detection of CAP, because the confirmation of suspect positive samples must be carried out by mass spectrometry (MS) coupled to chromatographic separation such as GC and HPLC, according to Commission Decision 2002/657/EC ().…”

Section: Introductionmentioning

confidence: 99%

Chloramphenicol Extraction from Honey, Milk, and Eggs Using Polymer Monolith Microextraction Followed by Liquid Chromatography−Mass Spectrometry Determination

Huang

Zhang

Feng

2006

J. Agric. Food Chem.

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A rapid confirmatory method for monitoring chloramphenicol (CAP) residues in honey, whole milk, and eggs is presented. This method is based on the polymer monolith microextraction (PMME) technique and high-performance liquid chromatography (HPLC)-electrospray ionization mass spectrometry (MS). A poly(methacrylic acid-ethylene glycol dimethacrylate) monolithic capillary column was selected as the extraction medium. To obtain optimum extraction efficiency, several parameters related to PMME were investigated. After dissolution in 20 mM phosphate solution at pH 4.0 and centrifugation, honey, eggs, or milk samples were directly passed through the extraction tube. The LC-MS instrument was equipped with an electrospray ion source and a single quadrupole. The eluates were analyzed by LC-MS in the negative-ion mode and by monitoring a pair of isotopic ions for the target compound. The in-source collision-induced dissociation process produced confirmatory ions. The recoveries of CAP from real samples spiked at 0.1-10 ng/g (honey), 0.2-10 ng/mL (milk), and 0.2-10 ng/g (egg) were in the range of 85-102%, with relative standard deviations ranging between 2.1% and 8.9%. The limits of detection (S/N = 3) were 0.02 ng/g, 0.04 ng/mL, and 0.04 ng/g in honey, milk, and eggs, respectively. The proposed method was proved to be robust in monitoring CAP residue in honey, milk, and eggs.

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Distribution of chloramphenicol residues in lactating cows following an external application

Cited by 18 publications

References 8 publications

Abusive use of antibiotics in poultry farming in Cameroon and the public health implications

Abusive use of antibiotics in poultry farming in Cameroon and the public health implications

Quantification of Antibiotic Residues and Determination of Antimicrobial Resistance Profiles of Microorganisms Isolated from Bovine Milk in Lebanon

Chloramphenicol Extraction from Honey, Milk, and Eggs Using Polymer Monolith Microextraction Followed by Liquid Chromatography−Mass Spectrometry Determination

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