Distribution of Cell Adhesion Molecules (Icam-1, Vcam-1, Elam-1) in Renal Tissue During Allograft Rejection

Brockmeyer, Carsten; Ulbrecht, Matthias; Schendel, Dolores J.; Weiß, Elisabeth H.; Hillebrand, Günther F.; Burkhardt, K; Land, W; Gokel, M; Riethmüller, Gert; Feucht, Helmut E.

doi:10.1097/00007890-199303000-00027

Cited by 128 publications

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“…4, 1998 ICAM-1, VCAM-1, and endothelial-leucocyte adhesion molecule-1 has been noted in renal tissue during renal allograft rejection, liver allograft rejection, and cytomegalovirus infection in the liver. 26,32 Of note, adhesion molecule expression was detected on renal parenchymal cells (tubular cells) as well as renal endothelial cells. 32 VCAM-1 expression has been reported in liver and pancreas allograft rejection on endothelial cells and infiltrating dendritic cells.…”

Section: Fig 3 Effect Of In Vivomentioning

confidence: 99%

“…26,32 Of note, adhesion molecule expression was detected on renal parenchymal cells (tubular cells) as well as renal endothelial cells. 32 VCAM-1 expression has been reported in liver and pancreas allograft rejection on endothelial cells and infiltrating dendritic cells. 33 Thus, adhesion molecule expression in human tissue has been detected both on endothelial, parenchymal, and other cell types during various inflammatory conditions.…”

Section: Fig 3 Effect Of In Vivomentioning

confidence: 99%

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Efficacy of anti-intercellular adhesion molecule-1 immunotherapy on immune responses to allogeneic hepatocytes in mice

Bumgardner

Heininger

et al. 1998

Hepatology

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Adhesion molecules appear to play important roles in vascularized organ allograft rejection, because antibodies directed against them are effective in prolonging survival of vascularized organ allografts in rodents. However, the efficacy of these agents for cellular allografts is unknown. The current studies were undertaken to determine the role of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on host immune responses to purified hepatocytes. Host mice (C3H, H-2 k ) grafted with hepatocytes in sponge matrix allografts (HC-SMA) received IgG isotype control, anti-ICAM-1, or anti-VCAM-1 monoclonal antibody (mAb) on days 0 through 9 after grafting. Twelve to 14 days later, host cells infiltrating the HC-SMA were assessed for the development of allospe- Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are expressed on endothelial and some parenchymal cells. These adhesion molecules (ICAM-1 and VCAM-1) also appear to play important roles in vascularized organ allograft rejection, because antibodies directed against ICAM-1 and VCAM-1 have been shown to be effective in prolonging survival of vascularized murine cardiac allografts, cynomolgous monkey renal transplants, and human renal transplants, [1][2][3][4][5][6] as well as nonvascularized skin allografts. 7 ICAM-1 has been implicated in liver transplant rejection by studies that show increased levels of soluble ICAM-1 during rejection episodes and histological detection of increased ICAM-1 during liver allograft rejection. [8][9][10] Parenchymal cells may constitutively express or may be induced to express adhesion molecules by various cytokines. However, the role of adhesion molecules in the presentation of major histocompatibility complex (MHC) antigens by parenchymal cells is not known. Cytokines, including interleukin-1, interferon gamma, and tumor necrosis factor (TNF), have been shown to up-regulate hepatocyte expression of ICAM-1 and their adhesion to T lymphocytes in vitro. 11 However, the in vivo effects of various cytokines on adhesion molecule expression in the liver has not been described, and the consequences of in vivo cytokine exposure upon the immunogenicity of liver cells has not been reported.Previously, we demonstrated that purified hepatocytes in the absence of donor-type antigen-presenting cells are immunogenic in vitro in mixed lymphocyte hepatocyte culture as determined by their ability to stimulate proliferation and the development of allospecific cytolytic effector T cells (alloCTLs) in mixed lymphocyte hepatocyte culture. The observed immunogenicity was correlated with the expression of MHC class I antigens. 12 We have further demonstrated that purified hepatocytes are immunogenic in vivo and stimulate the development of allo-CTLs in sponge matrix allografts (SMA) bearing allogeneic hepatocytes. 13,14 In this report, we extend our previous in vivo studies using the hepatocytesponge matrix allograft (HC-SMA) model to examine the role of ICA...

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Section: Fig 3 Effect Of In Vivomentioning

confidence: 99%

Section: Fig 3 Effect Of In Vivomentioning

confidence: 99%

Efficacy of anti-intercellular adhesion molecule-1 immunotherapy on immune responses to allogeneic hepatocytes in mice

Bumgardner

Heininger

et al. 1998

Hepatology

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“…Because of this activation, VCAM-1 has been implicated in the pathophysiology of certain autoimmune diseases, atherosclerosis, and allograft rejection. [3][4][5][6][7][8] VCAM-1 is constitutively expressed in bone marrow (BM) stromal/endothelial cells and certain classes of hematopoietic cells (B cells, follicular dendritic cells, and macrophages [9][10][11][12][13][14][15] ). Its major ligand is the integrin very late antigen 4 (VLA-4; ␣4␤1) with binding sites located in the first and fourth Ig domains, whereas other ligands bind with less affinity and include ␣4␤7, ␣9␤1, and ␣ D ␤2.…”

Section: Introductionmentioning

confidence: 99%

VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin

Ulyanova

Scott

Priestley

et al. 2005

Blood

138

117

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“…Multiple lines of evidence have emerged concerning the involvement of proximal tubular epithelial cells (TEC) in the renal immune response. These cells have been shown to express major histocompatibility complex (MHC) class II antigens, which are essential for antigen presentation to CD4 ϩ lymphocytes (32) and cellular adhesion molecules crucial for leukocyte migration, e.g., intercellular adhesion molecule-1 (16,18) and VCAM-1 (6). They are capable of processing and presenting foreign antigen (27) and, besides other cytokines, produce different chemokines, a group of low-molecular-weight cytokines with chemotactic functions.…”

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confidence: 99%

Interleukin-8 Secretion of Cortical Tubular Epithelial Cells Is Directed to the Basolateral Environment and Is Not Enhanced by Apical Exposure toEscherichia coli

Krüger¹,

Brandt

Klinger³

et al. 2000

Infect Immun

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More than 80% of urinary tract infections in adults are caused by Escherichia coli (31). For E. coli, different factors of virulence, e.g., lipopolysaccharides (LPS), hemolysins, or various types of fimbriae, have been characterized (8). Multiple lines of evidence have emerged concerning the involvement of proximal tubular epithelial cells (TEC) in the renal immune response. These cells have been shown to express major histocompatibility complex (MHC) class II antigens, which are essential for antigen presentation to CD4 ϩ lymphocytes (32) and cellular adhesion molecules crucial for leukocyte migration, e.g., intercellular adhesion molecule-1 (16, 18) and VCAM-1 (6). They are capable of processing and presenting foreign antigen (27) and, besides other cytokines, produce different chemokines, a group of low-molecular-weight cytokines with chemotactic functions. So far, the secretion of RANTES (14), MCP-1 (10), ENA-78 (28), and interleukin-8 (IL-8) (29) has been studied in TEC. Secretion of IL-8 is supposed to be of major relevance for the influx of neutrophils after bacterial contact. In earlier studies we showed that, in contrast to renal carcinoma cells (3), the expression of MHC class II molecules and intercellular adhesion molecule-1 by TEC could not be significantly enhanced by S fimbriae, LPS, or E. coli (21, 23). Furthermore, the secretion of IL-6, tumor necrosis factor alpha, and IL-8 by TEC grown as monolayers could be stimulated by cytokines, but not by S fimbriae, LPS, or E. coli (23). Concerning signalling of TEC directed to the basolateral environment, the in vitro model of monolayer cultures grown on a continuous surface has its limitations. Therefore, we tested whether basolaterally directed IL-8 secretion by TEC differs from luminal secretion and, if so, whether basolaterally directed IL-8 secretion can be stimulated by virulence factors of E. coli. MATERIALS AND METHODSPrimary cell culture of TEC and electron microscopy. Normal renal tissue was obtained in the local Department of Urology from nephrectomies due to tumors (23). Cells were grown from 1-mm 3 pieces of renal cortex in Dulbecco's modified Eagle medium-Ham's F-12 medium (BioWhittaker, Heidelberg, Germany) supplemented with epidermal growth factor (10 ng/ml), insulin-transferrin-sodiumselenite medium supplement (5 mg/liter), hydrocortisone (37.4 g/liter), 3,3-5-triiodo-L-thyronine (40 mg/liter), penicillin (100 U/ml), streptomycin (100 g/ ml), and 1 M HEPES buffer (15 ml/liter of medium). All supplements were obtained from Sigma, Deisenhofen, Germany, except for penicillin and streptomycin (BioWhittaker). The purity and proximal tubular origin of each cell culture were determined by immunohistochemistry by using anti-cytokeratin (Dianova, Hamburg, Germany), anti-APM (kindly provided by J. E. Scherberich, Frankfurt, Germany), anti-CD68, and anti-factor VIII antibodies (both from Dako, Hamburg, Germany) (21) and by electron microscopy. The ultrastructure of TEC with a microvillus surface is presented in Fig. 1. For electron microscopy, sma...

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Distribution of Cell Adhesion Molecules (Icam-1, Vcam-1, Elam-1) in Renal Tissue During Allograft Rejection

Cited by 128 publications

References 0 publications

Efficacy of anti-intercellular adhesion molecule-1 immunotherapy on immune responses to allogeneic hepatocytes in mice

Efficacy of anti-intercellular adhesion molecule-1 immunotherapy on immune responses to allogeneic hepatocytes in mice

VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin

Interleukin-8 Secretion of Cortical Tubular Epithelial Cells Is Directed to the Basolateral Environment and Is Not Enhanced by Apical Exposure toEscherichia coli

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