Distribution of CCK mRNA in particular regions (hippocampus, periaqueductal grey and thalamus) of the rat by in situ hybridization

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

“…The discrepancy between in situ hybridization and immunohistochemistry with regard to CCK in sensory neurons and in spinal motoneurons is not surprising, since a similar situation has been reported in many brain regions. For example, a very large population of thalamic and cortical neurons, including pyramidal neurons, express CCK mRNA (Burgunder and Young, 1988;Savasta et al, 1988;Voigt and Uhl, 1988;Ingram et al, 1989;Lanaud et al, 1989;Seroogy et al, 1989;CortCs et al, 1990b;Schiffinann and Vanderhaeghen, 1991), but only a small population of cortical interneurons and no corresponding thalamic neurons have so far been shown to express CCK-LI with immunohistochemistry (Innis et al, 1979;Larsson and Rehfeld, 1979;LorCn et al, 1979;Vanderhaeghen et al, 1980;McDonald et al, 1982;Cho et al, 1983;Hendry et al, 1983;Peters et al, 1983;Hokfelt et al, 1988; for discussion see Hokfelt et al, 1991).…”

Vasoactive Intestinal Peptide Selectively Depolarizes Thalamic Relay Neurons and Attenuates Intrathalamic Rhythmic Activity

“…A variety of neuropeptides and their receptors are localized within the thalamus (Baldino et al 1989;Burgunder and Young 1988;Doetsch et al 1993;Graber and Burgunder 1996;Kaneko and Mizuno 1988;Lanaud et al 1989;Molinari et al 1987;Staun-Olsen et al 1985). The functional significance of these modulators within thalamic nuclei has remained somewhat elusive.…”

Periaqueductal Gray

“…For example, VIP and galanin staining is restricted to the juxta-aqueductal zone Melander et al, 1986aMelander et al, , 1986b; phenylethanolamine N-methyltransferase and calcitonin staining are particularly intense within parts of the vlPAG (Fabbri et al, 1985;Herbert and Saper, 1992); calbindin and to a large extent calretinin staining is most pronounced in the dlPAG, the vlPAG and the ventral part of the lPAG, but absent in the dorsal part of the lPAG and the dmPAG (Paxinos et al, 1999); neurofilament protein SMI-32 staining is present in the outer (lateral) zone of dmPAG, lPAG and vlPAG, but absent in the inner (medial) zone of these columns and in the dlPAG (Paxinos et al, 1999). In addition, the distribution within the PAG of low abundance peptides, transmitter substances and their receptors, have been studied using in situ hybridization (Harlan et al, 1987;Lanaud et al, 1989;Seroogy et al, 1989;De Belleroche et al, 1990;Rattray et al, 1992;Tolle et al, 1993;Mansour et al, 1994Mansour et al, , 1995Smith et al, 1994;Jansen et al, 1998) and receptor autoradiographic techniques (Williams and Beitz, 1989a, 1989bGundlach, 1991). These studies have revealed additional discrete inter-and intra-columnar patterns of substance-specific cellular labels (mRNAs) and receptors (silver grain densities).…”