Distribution of Carcinogenic Human Papillomavirus Genotypes and Association to Cervical Lesions among Women in Fez (Morocco)

Souho, Tiatou; Fatemi, Hinde El; Karim, Safae; Rhazi, K. El; Bouchikhi, C.; Banani, Abdelaziz; Melhouf, Moulay Abdelilah; Benlemlih, Mohammed; Bennani, Bahia

doi:10.1371/journal.pone.0146246

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…In this study, we found a significantly higher 481 prevalence of HPV infection in women living with HIV/AIDS (74.50%) and HR-HPV types were present in the vast majority of cases (75%). These findings highlighted that the HPV prevalence in the HIVpositive Moroccan women was significantly higher than observed in the general population (15.7%-43.1%) [6][7][8]. This seems to be in line with other previous reports which documents high proportion of HIVinfected women carrying varieties of HR-HPV types [27,28].…”

Section: Discussionsupporting

confidence: 90%

“…This result seems to be in line with previous reports [36,44]. In contrast, this molecular epidemiology profile was not consistent with observed in HIV-negative women [6][7][8] or women with CC in Morocco [9][10][11][12], in whom HPV 16 and 18 predominate. Such discrepancies between groups (HIVpositive and HIV-negative) has been reported in previous studies and may partly due to immune system deficiency [30,45].…”

Section: Discussionsupporting

confidence: 73%

“…This heterogenous virus family includes more than 200 genotypes [5]. Previous studies in Moroccan women have been reported an inconsistent prevalence of HPV with 15.7% [6] in Rabat and 42.5-43.1% in Fez region [7,8]. However, the prevalence was much higher, ranging from 62% to 92%, among Moroccan women with CC [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

Ouladlahsen¹,

Fayssel

Bensghir³

et al. 2018

J Infect Dev Ctries

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Introduction: Women infected with human immunodeficiency virus (HIV) have a higher risk of contracting human papillomavirus (HPV) infections and are more prone to develop cervical cancer. The objective of this study was to determine the prevalence of HPV and its association with risk factors among Moroccan women living with HIV/AIDS. Methodology: We enrolled 251 HIV-infected non-pregnant women in Morocco from February 2013 to September 2016. Sociodemographic, lifestyles, behavioral and clinical data were collected. Polymerase chain reaction followed by sequencing were performed for molecular detection and HPV genotyping in cervical samples, respectively. Results: Abnormal cervical smears were found in 34/246 patients (13.82%). The overall prevalence of HPV was 74.50%. HPV 58 was the most prevalent (39.29%) followed by HPV 18 (10.71%), HPV 70 (8.93%), HPV 33 (7.14%), HPV 6 (6.25%) and other genotypes (< 3%). Overall, high-risk HPV (HR-HPV) types were present in 75% (84/112) of patients and the prevalence of HR-HPV types in samples with abnormal Pap was higher than in normal Pap (55/83, 66.27% vs. 28/83,33.33%, p < 0.0001). Univariate analyses showed that none of the socio-demographic and behaviors factors was associated with HPV infection. Moreover, Pap results were not affected by HPV status (p = 0.532). Whereas, CD4 T-cell counts above 200/mm 3 at enrolment were apparently not protective to HPV infection. We found a high prevalence of HPV infection and HR-HPV types among HIV-positive women that significantly associated with abnormal Pap. Conclusion: Our findings suggest a high prevalence of HPV infection with high-risk types was observed among HIV-positive women warrant to implement a regular screening by Pap smear.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 73%

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The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

Ouladlahsen¹,

Fayssel

Bensghir³

et al. 2018

J Infect Dev Ctries

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“…However, because of the cultural traditions, the limitation of this study is the lack of data about the sexual partner, extramarital relationships (for wives and husbands), sexual life and practice that are still taboo subjects. Considering women with normal cervical cytology, when we compared the overall HPV prevalence among Tunisian women with that from neighboring Northern African countries, we reported that it was higher than that of Algeria (5.3%) [22], but lower than that of Morocco (34.3%) [30]. Additionally, we found relatively high HPV prevalence compared to Arab-Muslim countries from the Middle-East [31–33] but substantially lower than that reported by other studies of the Sub-Saharan countries [34, 35] and Mediterranean Europe [36, 37].…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus genotypes and HPV-16 variants distribution among Tunisian women with normal cytology and squamous intraepithelial lesions

Ghedira

Mahfoudh

Hadhri³

et al. 2016

Infect Agents Cancer

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BackgroundLittle is known about the epidemiological characteristics of papillomavirus (HPV) infection among North African countries. Herein, we conducted a molecular epidemiological study to investigate prevalence of HPV type and HPV-16 variants among cervical-screened unvaccinated Tunisian women.MethodsCross-sectional study was performed on 494 Tunisian women visiting Women’s Healthcare Centers. HPV-DNA detection was carried out on cervical samples using real-time polymerase chain reaction. HPV genotyping and HPV-16 variants were characterized by direct sequencing of L1 viral capsid gene.ResultsThe overall HPV prevalence was 34% (95% CI: 30–38%) with significantly higher prevalence among women with squamous intraepithelial lesions (SIL) than those with no intraepithelial lesions (NIL) 84% (95% CI: 76–92%) and 24.5% (95% CI: 20–29%) respectively. The distribution of HPV prevalence according to women’s age shows a U-shaped curve and the highest HPV prevalence rates were observed among the youngest (≤25 years; 51.2%, 95% CI: 37–67%) and the oldest women (>55 years; 41.7%, 95% The HPV-16 prevalence was 32.8% (95% CI: 22–45%) among women with SIL and 9.2% (95% CI: 6–12%) among women with NIL. Whereas, the HPV-18 prevalence was 1.3% (95% CI: 0–5%) among women with SIL and 0.3% (95% CI: 0–1%) among women with NIL. Among HPV-16 positive women, European lineage (E) was identified as the predominant HPV-16 variant (85.7%, 95% CI: 76–95%). The frequency of E variant was lower among SIL than among NIL women (81%, 95% CI: 64–99%, and 88%, 95% CI: 77–100%, respectively). Conversely, the African-2 variant frequency was higher among SIL than among NIL women (18%, 95% CI: 1–36% and 6%, 95% CI: 2–14%, respectively). In multivariate analysis, young age was the only risk factor that is independently associated with HPV infection. Moreover, HPV infection and menopause were both found to be independently associated with SIL and HSIL.ConclusionHPV DNA testing should be proposed to young and menopausal Tunisian women. Considering HPV prevalence, only 13% of the Tunisian women could be protected by the bivalent HPV vaccine. These results may be helpful for designing an adapted HPV testing and vaccination program in Tunisia.

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“…Certain strains of HPV are associated with different risk levels of transformation into genital tract in both males and females (13,(15)(16)(17). High-risk HPV16 and HPV18, which are found in high frequencies in cervical cancer, account for approximately two-thirds of all cervical carcinomas worldwide (HPV16 as the most frequent type) (16)(17)(18)(19)(20)(21)(22). associated with an increased risk of cervical cancer development (23).…”

Section: Introductionmentioning

confidence: 99%

The Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Women with Normal Cytology in North of Iran

Daneshvar

Haghshenas

Majleci

et al. 2016

Jundishapur J Microbiol

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Background: Cervical cancer is a leading cause of mortality in women. Infection with high-risk human papillomavirus (HPV) is recognized as an important etiological factor for the development of cervical cancer. On the other hand, cervical cancer screening can detect abnormal cervical cells and diagnose cervical cancer in the early stages. Objectives: This study was performed to determine and analyse the distribution of HPV genotypes in normal cervical tissues, using polymerase chain reaction in Mazandaran province in North of Iran. Methods: This cross-sectional study was performed during 2012 -2015. Tissue samples from the cervix were collected from 450 women, referring to a healthcare centre in Mazandaran province, Iran. Pap smear was used to monitor any abnormalities or unusual findings. Also, GP5 + / GP6 + primers were applied for the detection of HPV DNA in the specimens. Afterwards, positive samples were selected to perform high-risk HPV genotyping, using the HPV HCR DNA genotyping kit. Results: In this study, 431 (95.78%) samples were found to be normal on the Pap smear test, while 19 (4.22%) samples were abnormal. We found that the rate of positive HPV DNA was 10.22% in the tissue cervix samples. Human papillomavirus DNA was detected in 8.12% (35 of 431) of the normal tissues and 57.89% (11 of 19) of the abnormal tissues. From the 46 HPV DNA positive samples, 19 (41.30%) were HPV16, 9 (19.57%) of which were HPV18 and 1 (2.17%) was HPV45. In this study, the highest prevalence of HPV DNA was found in women within the age range of 20-24 years, which constitutes 20 (28.17%) of the HPV DNA positive samples. Conclusions: Cervical cancer screening, comprising of both Pap test and HPV testing, is an essential part of the routine health care for women as it can facilitate the detection of abnormal cervical cells. HPV infection can produce abnormal Pap test results; also, certain types of HPV are associated with cancer. Therefore, it is important to diagnose abnormal cervical tissues before the development of cervical cancer.

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Distribution of Carcinogenic Human Papillomavirus Genotypes and Association to Cervical Lesions among Women in Fez (Morocco)

Cited by 10 publications

References 34 publications

The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

Human papillomavirus genotypes and HPV-16 variants distribution among Tunisian women with normal cytology and squamous intraepithelial lesions

The Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Women with Normal Cytology in North of Iran

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