Distribution of capsular materials on the cell wall surface of strain Smith diffuse of Staphylococcus aureus

Arizono, Takeshi; Umeda, Akira; Amako, Kazunobu

doi:10.1128/jb.173.14.4333-4340.1991

Cited by 17 publications

(18 citation statements)

References 24 publications

(29 reference statements)

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“…It seems likely that this scenario is due to the presence of bacteria without capsules as well as capsulated cells in the cultures. Bacterial cultures may thus contain a heterogeneous mixture of cell populations with more or less intact capsules even when optimal conditions have been used for culturing (30), and postincubation processing of bacterial cells may further damage capsulation (2). Heterogeneity among cells in the same culture was demonstrated in the present study by flow cytometric analysis of bacterial cells (Fig.…”

Section: Discussionmentioning

confidence: 61%

Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin

et al. 2005

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Mannan-binding lectin (MBL), L-ficolin, and H-ficolin are pattern recognition molecules of the innate immune system. We investigated their ability to bind to different serotypes and noncapsulated variants of two gram-positive bacterial species, Streptococcus pneumoniae and Staphylococcus aureus. MBL did not bind to capsulated S. aureus or capsulated S. pneumoniae but did bind to a noncapsulated S. aureus variant (Wood). L-ficolin bound to some capsulated S. aureus serotypes (serotypes 1, 8, 9, 11, and 12) and capsulated S. pneumoniae serotypes (11A, 11D, and 11F) but not to noncapsulated strains. H-ficolin did not bind to any of the S. pneumoniae and S. aureus serotypes included in this study but did bind to one strain of Aerococcus viridans. The concentrations of the three proteins in 97 plasma samples were estimated. The median concentrations were 0.8 g per ml for MBL, 3.3 g per ml for L-ficolin, and 18.4 g per ml for H-ficolin.

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Section: Discussionmentioning

confidence: 61%

Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin

et al. 2005

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“…It was postulated that this fibrous layer reflects a region of autolysin-catalyzed degradation of outer nonstressed peptidoglycan (189). A similar organization was also observed in many strains of S. aureus (23,478). This topography is illustrated in Fig.…”

Section: Topography Of Tasmentioning

confidence: 89%

A Continuum of Anionic Charge: Structures and Functions ofd-Alanyl-Teichoic Acids in Gram-Positive Bacteria

Neuhaus

Baddiley

2003

Microbiol Mol Biol Rev

901

1,097

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SUMMARY Teichoic acids (TAs) are major wall and membrane components of most gram-positive bacteria. With few exceptions, they are polymers of glycerol-phosphate or ribitol-phosphate to which are attached glycosyl and d-alanyl ester residues. Wall TA is attached to peptidoglycan via a linkage unit, whereas lipoteichoic acid is attached to glycolipid intercalated in the membrane. Together with peptidoglycan, these polymers make up a polyanionic matrix that functions in (i) cation homeostasis; (ii) trafficking of ions, nutrients, proteins, and antibiotics; (iii) regulation of autolysins; and (iv) presentation of envelope proteins. The esterification of TAs with d-alanyl esters provides a means of modulating the net anionic charge, determining the cationic binding capacity, and displaying cations in the wall. This review addresses the structures and functions of d-alanyl-TAs, the d-alanylation system encoded by the dlt operon, and the roles of TAs in cell growth. The importance of dlt in the physiology of many organisms is illustrated by the variety of mutant phenotypes. In addition, advances in our understanding of d-alanyl ester function in virulence and host-mediated responses have been made possible through targeted mutagenesis of dlt. Studies of the mechanism of d-alanylation have identified two potential targets of antibacterial action and provided possible screening reactions for designing novel agents targeted to d-alanyl-TA synthesis.

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“…Teichoic acid was purified from CWC extracted from the cell by lysostaphin digestion without being purified from the cell wall fraction extracted from the disintegration of the cell with the methods of Arizono et al (4) and Umeda et al (27). This lysostaphin digestion was performed in Tris-HCl buffer containing 30% (w/v) raffinose to protect the protoplast from digestion, so the extracted CWC is thought to exclude intracellular components and may be purer than the above cell wall fraction.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Teichoic Acid‐Like Substance as an Adhesin of Staphylococcus aureus to HeLa Cells

Matsuura

Miyake

Nakashima

et al. 1996

Microbiology and Immunology

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A cell wall component that bound to HeLa cells (HeLa cell-binding CWC) was isolated from a clinical isolate of Staphylococcus aureus. The HeLa cell-binding CWC was resistant to heat (100 C, 1 hr) and proteases, did not stain with Coomassie Brilliant Blue R-250 on SDS-PAGE but stained as a broad band with antiserum against the strain on Western blots. These data suggest that the HeLa cell-binding CWC is not a protein, and may be teichoic acid. Purified teichoic acid bound to HeLa cells, whereas fractions without teichoic acid did not. In Western blots, HeLa cell-binding CWC appeared as a broad band of less than 35 kDa, similar to that of purified teichoic acid. These data suggest that the HeLa cell-binding CWC obtained in this study is teichoic acid. Teichoic acid inhibited S. aureus adherence to HeLa cells and bound to the cells time and dose dependently, in a saturable and reversible manner, and therefore appears to be an adhesin of S. aureus to HeLa cells.

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Distribution of capsular materials on the cell wall surface of strain Smith diffuse of Staphylococcus aureus

Cited by 17 publications

References 24 publications

Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin

Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin

A Continuum of Anionic Charge: Structures and Functions ofd-Alanyl-Teichoic Acids in Gram-Positive Bacteria

Isolation and Characterization of Teichoic Acid‐Like Substance as an Adhesin of Staphylococcus aureus to HeLa Cells

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